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pLENTI_TetOn_Hu_BIRC5(WT)

Plasmid

#136348

Purpose

Lentiviral inducible expression (TetOn) of human BIRC5

Depositor

Guillermo de Cárcer

Insert

BIRC5 (BIRC5 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-EGFR

Plasmid

#65225

Purpose

Expression of EGFR in mammalian cells

Depositor

Axel Ullrich

Insert

EGFR (EGFR Human)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pCGN-MEF2D

Plasmid

#32963

Purpose

For mammalian expression of mouse MEF2D with an HA tag

Depositor

Ron Prywes

Insert

MEF2D (Mef2d Mouse)

Tags

HA

Expression

Mammalian

Mutation

E287G

Promoter

CMV

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pH6-MBP-TEV-BirA

Plasmid

#179694

Purpose

Expresses His6 tagged MBP-BirA fusion protein

Depositor

Mark Arbing

Insert

BirA

Tags

His6-MBP

Expression

Bacterial

Available Since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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PB CAG-tdTomato

Plasmid

#133569

Purpose

CAG tdTomato piggyBac transposon

Depositor

Mario Capecchi

Insert

tdTomato

Expression

Mammalian

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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PB CAG-sfGFP

Plasmid

#133568

Purpose

CAG sfGFP piggyBac transposon

Depositor

Mario Capecchi

Insert

sfGFP

Expression

Mammalian

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV CCTG 1200x

Plasmid

#63088

Purpose

AdenoAssociated Virus plasmid containing expanded repeats--CCTG 1200x that correspond to Myotonic Dystrophy of type 2

Depositor

Nicolas Charlet-Berguerand

Insert

CCUG 1200x

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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pJJR81

Plasmid

#75029

Purpose

tagBFP2^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Mike Boxem

Insert

tagBFP2^SEC^3xFlag

Use

CRISPR

Tags

3xFLAG and C. elegans codon optimized tagBFP2

Expression

Worm

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-dCas9-VPR-H2B-GFP (CRISPRa)

Plasmid

#175571

Purpose

Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation

Depositor

Joseph Brzezinski

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pTRACER LA

Plasmid

#15003

Depositor

Raymond Birge

Insert

LA (MFGE8 Human)

Tags

6xHis and V5

Expression

Mammalian

Available Since

Aug. 22, 2007

Availability

Academic Institutions and Nonprofits only

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pGEX 6P1 CDK2 (552)

Plasmid

#61845

Purpose

bacterial expression of GST CDK2 WT

Depositor

Jonathon Pines

Insert

CDK2 (CDK2 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only
p405ADH1

Plasmid

#15966

Depositor

Nicolas Buchler, Fred Cross

Insert

p405ADH1 (ADH1 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

ADH1 promoter is between SacI and XbaI restrictio…

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pENTR_mCherry

Plasmid

#139554

Purpose

mCherry ORF with stop codon between attL1-attL2 on Gateway entry vector

Depositor

Minoru Ko

Insert

mCherry

Expression

Mammalian

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAd/CMV/DAX1-IRES-nEGFP

Plasmid

#29752

Depositor

Edward McCabe

Insert

dosage-sensitive sex reversal, adrenal hypoplasia critical region, on chromosome X, gene 1 (NR0B1 Human)

Tags

EGFP, IRES, and Nuclear localization sequence

Expression

Mammalian

Mutation

No mutations; Reporter is nuclear localized EGFP;…

Available Since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only

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HA-TSC2

Plasmid

#24939

Depositor

Kunliang Guan

Insert

TSC2 (Tsc2 Rat)

Use

N/a

Tags

3xHA

Available Since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only
pJA281

Plasmid

#22513

Depositor

Julie Ahringer

Article

Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

Insert

Pmex-5::mCherry (w introns w/o stop)

Expression

Worm

Available Since

March 16, 2010

Availability

Academic Institutions and Nonprofits only

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pWS172

Plasmid

#90519

Purpose

Cas9 gap repair vector - HIS3

Depositor

Tom Ellis

Insert

Cas9

Use

CRISPR

Expression

Yeast

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti Lifeact-mTagBFP2 PuroR

Plasmid

#101893

Purpose

Lentiviral expression of mTagBFP2-tagged Lifeact with Puromycin selection in cells including neurons

Depositor

Ghassan Mouneimne

Insert

Lifeact

Use

Lentiviral

Tags

mTagBFP2

Promoter

CMV immediate early

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pQCXIZ (w298-1)

Plasmid

#22801

Depositor

Eric Campeau

Type

Empty backbone

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Dec. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3 mTSP2

Plasmid

#12411

Depositor

Paul Bornstein

Insert

TSP2 (Thbs2 Mouse)

Expression

Mammalian

Available Since

Oct. 23, 2006

Availability

Academic Institutions and Nonprofits only