We narrowed to 17,931 results for: COMP
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Plasmid#223389PurposeT-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for monocot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter.DepositorInsertZmUbi-hA3A-Y130F-SpCas9-D10A-UGI-OsU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT23
Plasmid#223395PurposeT-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for monocot plants; NG or NA PAM ; wide working window; A3A/Y130F-CBE_V01 was driven by ZmUbi1 and sgRNA was driven by OsU3 promoter.DepositorInsertZmUbi-hA3A-Y130F-SpRYD10A-UGI-OsU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT26
Plasmid#223398PurposeT-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for dicot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by ZmUbi1 and the sgRNA was driven by AtU3; BASTA for plants select.DepositorInsertZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-AtU3-gRNA scaffold 2.0
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT28
Plasmid#223400PurposeT-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by ZmUbi1 and sgRNA was driven by OsU3; Hygromycin for plant select.DepositorInsertZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-OsU3-gRNA scaffold 2.0
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT29
Plasmid#223401PurposeT-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by ZmUbi1 and the sgRNA was driven by OsU3; BASTA for plant select.DepositorInsertZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-OsU3-gRNA scaffold 2.0
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT30
Plasmid#223402PurposeT-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 and the sgRNA was driven by separate ZmUbi1; Hygromycin for plants selection.DepositorInsertZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-ZmUbi-gRNA scaffold 2.0-tRNA
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT14
Plasmid#223386PurposeT-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by ZmUbi1 and sgRNA was by AtU3; BASTA for plant selection.DepositorInsertZmUbi-hA3A-Y130F-SpCas9-D10A-UGI-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
1010Av2
Plasmid#125642PurposeExpresses FAR protein under 10xUAS controlDepositorInsertFAR protein
UseTagsExpressionInsectMutationPromoter10xUASAvailable sinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
1010Bv2
Plasmid#125643PurposeExpresses FAR protein under 10xUAS controlDepositorInsertFAR protein
UseTagsExpressionInsectMutationPromoter10xUASAvailable sinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN_N73_eMagBF_V5
Plasmid#235642PurposeEncodes ALIBi N-terminal fragment, residues 1-73, fused to eMagBF (enhanced Magnet, basic, fast), V5. No nuclear export sequence to allow nuclear localization.DepositorInsertALIBi N-terminal fragment
UseTagseMagBF-V5ExpressionMammalianMutationresidues 1-73PromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN_Fbl_N73_eMagBF_V5
Plasmid#235643PurposeEncodes ALIBi N-terminal fragment, residues 1-73, fused to eMagBF (enhanced Magnet, basic, fast), V5, Fbl for targeting to nucleolus.DepositorInsertALIBi N-terminal fragment
UseTagsFbl-N73- and eMagBF-V5ExpressionMammalianMutationresidues 1-73PromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
PX459_L31_CRISPR_downstream_sgRNA
Plasmid#235610PurposeEncodes CRISPR gRNA for cleaving downstream of insertion site at C terminus of mouse Rpl31DepositorInsertRpl31 C-terminus downstream sgRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
PX459_L31_CRISPR_upstream_sgRNA
Plasmid#235609PurposeEncodes CRISPR gRNA for cleaving upstream of insertion site at C terminus of mouse Rpl31DepositorInsertRpl31 C-terminus upstream sgRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN_N73_eMagBF_V5_NES
Plasmid#235614PurposeEncodes ALIBi N-terminal fragment, residues 1-73, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.DepositorInsertALIBi N-terminal fragment
UseTagseMagBF-V5-NESExpressionMammalianMutationresidues 1-73PromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN_NES_V5_BirA
Plasmid#235634PurposeEncodes full-length BirA fused to a nuclear export sequence and V5. Used as a positive control in ALIBi experimentsDepositorInsertBirA
UseTagsNES-V5ExpressionMammalianMutationPromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN_N65_eMagBF_V5_NES
Plasmid#235612PurposeEncodes ALIBi N-terminal fragment, residues 1-65, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.DepositorInsertALIBi N-terminal fragment
UseTagseMagBF-V5-NESExpressionMammalianMutationresidues 1-65PromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN_N77_eMagBF_V5_NES
Plasmid#235616PurposeEncodes ALIBi N-terminal fragment, residues 1-77, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.DepositorInsertALIBi N-terminal fragment
UseTagseMagBF-V5-NESExpressionMammalianMutationresidues 1-77PromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN_N99_eMagBF_V5_NES
Plasmid#235618PurposeEncodes ALIBi N-terminal fragment, residues 1-99, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.DepositorInsertALIBi N-terminal fragment
UseTagseMagBF-V5-NESExpressionMammalianMutationresidues 1-99PromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80EN10-Pct3-(–)-Eldh1(cHis6)-noAccs(ElA2)
Plasmid#233880PurposeExpresses His-tagged Eggerthella lenta A2 (–)-Eldh1 with a cumate inducible promoter, without accessory genes (inactive), in Gordonibacter urolithinfaciens DSM 27213DepositorInsert(–)-Eldh1 (Elen_0511 Eggerthella lenta)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80EN8-Pct3-(+)-Eddh(cHis6)-noAccs(ElA2)
Plasmid#233879PurposeExpresses His-tagged Eggerthella lenta A2 (+)-Eddh with a cumate inducible promoter, without accessory genes (inactive), in Gordonibacter urolithinfaciens DSM 27213DepositorInsert(+)-Eddh (Elen_0501 Eggerthella lenta)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80ENSF26-Pct3-(+)-Eldh1(nHis6)(GpDSM19378)
Plasmid#233874PurposeExpresses His-tagged Gordonibacter pamelaeae DSM 19378 (+)-Eldh1 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purificationDepositorInsert(+)-Eldh1 (GPA_25810 Gordonibacter pamelaeae DSM 19378)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80ENSF14-Pct3-ElVadh(cHis6)-Accs(ElDSM2243)
Plasmid#233871PurposeExpresses His-tagged Eggerthella lenta DSM 2243 El Vadh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purificationDepositorInsertEl Vadh (Elen_0497 Eggerthella lenta)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80ENSF12-Pct3-(–)-Eldh1(cHis6)-Accs(ElA2)
Plasmid#233870PurposeExpresses His-tagged Eggerthella lenta A2 (–)-Eldh1 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purificationDepositorInsert(–)-Eldh1 (Elen_0511 Eggerthella lenta)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80ENSF10-Pct3-(+)-Eddh(cHis6)-Accs(ElA2)
Plasmid#233869PurposeExpresses His-tagged Eggerthella lenta A2 (+)-Eddh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purificationDepositorInsert(+)-Eddh (Elen_0501 Eggerthella lenta)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
XZ093 SFFV-sensor-IRESv0-LIDARst(IRESv0-AVPR2)
Plasmid#233430PurposeLIDAR all components on single transcript (as shown in Figure 4A), using EMCV IRES to separate each componentDepositorInsertsensor-IRESv0-LIDARst(IRESv0-AVPR2)
UseTagsExpressionMammalianMutationE488Q, T501A on ADAR2ddPromoterSFFVAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD70EN19-Pct5-GPA_25810/25800-(+)-Eldh1/2(GpDSM19378)
Plasmid#225305PurposeExpresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh1 and (+)-Eldh2 in Eggerthella lenta DSM 2243 with a cumate inducible promoterDepositorInsert(+)-Eldh1, (+)-Eldh2
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80ENSF27-Pct3-(+)-Eldh2(nHis6)(GpDSM19378)
Plasmid#233875PurposeExpresses His-tagged Gordonibacter pamelaeae DSM 19378 (+)-Eldh2 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purificationDepositorInsert(+)-Eldh2 (GPA_25800 Gordonibacter pamelaeae DSM 19378)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-CasRx-HA-mCherry-pA
Plasmid#233028PurposeTo Express HA tagged CasRX-mCherry fusion protein from the mammalian EFS promoterDepositorInsertCasRx-mCherry
UseAAVTagsmCherryExpressionMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFHΔ36-72
Plasmid#232060PurposeExpresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 36-72DepositorInsertNeurofilament-Heavy tail domain lacking residues 36-72
UseTags6xHisExpressionBacterialMutationadded N-terminal tryptophan and tyrosine, and cys…PromoterAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFHΔ462-638
Plasmid#232061PurposeExpresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 462-638DepositorInsertNeurofilament-Heavy tail domain lacking residues 462-638
UseTags6xHisExpressionBacterialMutationadded N-terminal tryptophan and tyrosine, and cys…PromoterAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFLtail
Plasmid#232055PurposeExpresses construct for surface tethering: R. norvegicus NFL tail domainDepositorInsertNeurofilament-Light tail domain
UseTags6xHisExpressionBacterialMutationcDNA codon-optimized for E. coli; added N-termina…PromoterT7Available sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFMtail
Plasmid#232056PurposeExpresses construct for surface tethering: R. norvegicus NFM tail domainDepositorInsertNeurofilament-Medium tail domain
UseTags6xHis, thrombinExpressionBacterialMutationcDNA codon-optimized for E. coli; added N-termina…PromoterT7Available sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFMshuf1
Plasmid#232058PurposeExpresses construct for surface tethering: R. norvegicus NFM tail domain with more evenly distributed charged residues, sequence 1DepositorInsertNeurofilament-Medium tail domain, charge shuffle 1
UseTags6xHis, thrombinExpressionBacterialMutationPromoterAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFHtail
Plasmid#232057PurposeExpresses construct for surface tethering: R. norvegicus NFH tail domainDepositorInsertNeurofilament-Heavy tail domain
UseTags6xHisExpressionBacterialMutationadded N-terminal tryptophan and tyrosine, and cys…PromoterAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFMshuf2
Plasmid#232059PurposeExpresses construct for surface tethering: R. norvegicus NFM tail domain with more evenly distributed charged residues, sequence 2DepositorInsertNeurofilament-Medium tail domain, charge shuffle 2
UseTags6xHis, thrombinExpressionBacterialMutationPromoterAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNDGG006
Plasmid#231310PurposeVector part for BEVA; BEVA2.0 Position 3 p15A narrow host range origin of replication with oriT, CmR.DepositorInsertBEVA2.0 Position 3 p15A narrow host range origin of replication with oriT, CmR.
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNDGG007
Plasmid#231311PurposeVector part for BEVA; BEVA2.0 Position 3 pMB1 narrow broad host range origin of replication with oriT, CmR.DepositorInsertBEVA2.0 Position 3 pMB1 narrow broad host range origin of replication with oriT, CmR.
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNDGG010
Plasmid#231315PurposeVector part for BEVA; BEVA2.0 Position 4 attB recombinase site, AmpR. Also includes FRT EF module for L1 GG cloning.DepositorInsertBEVA2.0 Position 4 attB recombinase site, AmpR. Also includes FRT EF module for L1 GG cloning.
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNDGG022
Plasmid#231306PurposeVector part for BEVA; BEVA2.0 Position 1 Level 1 BsaI cloning site with alternate CAGA 3' fusion site, without T0, AmpRDepositorInsertBEVA2.0 Position 1 Level 1 BsaI cloning site with alternate CAGA 3' fusion site, without T0, AmpR
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNDGG009
Plasmid#231314PurposeVector part for BEVA; BEVA2.0 Position 4 FRT recombinase site, AmpR. Also includes attB EF module for L1 GG cloning.DepositorInsertBEVA2.0 Position 4 FRT recombinase site, AmpR. Also includes attB EF module for L1 GG cloning.
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNDGG002
Plasmid#231308PurposeVector part for BEVA; BEVA2.0 Position 2 Sp antibiotic resistance via aadA, AmpR/SpRDepositorInsertBEVA2.0 Position 2 Sp antibiotic resistance via aadA, AmpR/SpR
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNDGG001
Plasmid#231307PurposeVector part for BEVA; BEVA2.0 Position 2 Km/Nm antibiotic resistance via nptII, CmR/KmR/NmR.DepositorInsertBEVA2.0 Position 2 Km/Nm antibiotic resistance via nptII, CmR/KmR/NmR.
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
plko_TRE3G_Myc-Sox9HMGonlySTOP_PGKpuroR
Plasmid#203963PurposeDox inducible myc tag truncated SOX9 without trans-activating domain (noTA)DepositorInsertSOX9-noTA (Sox9 Mouse)
UseLentiviralTagsmycExpressionMutationthe TA domain is truncatedPromoterTRE3GAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
plko_TRE3G_nlsMyc-Sox9HMGtruncation_PGKpuroR
Plasmid#203964PurposeDox inducible myc tag truncated SOX9 without DNA-binding domain (noHMG)DepositorInsertSOX9-noHMG (Sox9 Mouse)
UseLentiviralTagsmycExpressionMutationthe HMG domain is truncatedPromoterTRE3GAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Ydf NanoLuc
Plasmid#225656PurposeReporter plasmid expressing NanoLuciferase under the control of the promoter PydfJ115, cognate to YdfI. Responds to NarL/NarX chimeras from this study.DepositorInsertNanoLuciferase
UseCell free expressionTagsMycExpressionBacterialMutationPromoterPydfJ115Available sinceDec. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL CusS NarX Chimera
Plasmid#225652PurposeExpresses a NarX-based chimera capable of sensing copper (II). For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertCusS-NarX
UseCell free expressionTagsMycExpressionBacterialMutationTransmembrane and sensing domain replaced to dete…PromoterT7Available sinceDec. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL NrsS NarX Chimera
Plasmid#225653PurposeExpresses a NarX-based chimera capable of sensing nickel (II). For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertNrsS-NarX
UseCell free expressionTagsMycExpressionBacterialMutationTransmembrane and sensing domain replaced to dete…PromoterT7Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL RssA NarX Chimera
Plasmid#225654PurposeExpresses a NarX-based chimera capable of sensing iron (III). For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertRssA-NarX
UseCell free expressionTagsMycExpressionBacterialMutationTransmembrane and sensing domain replaced to dete…PromoterT7Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL NarX Myc
Plasmid#225651PurposeExpresses WT NarX capable of sensing nitrate. For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertNarX (narX E. coli)
UseCell free expressionTagsMycExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL VanS NarX Chimera
Plasmid#225655PurposeExpresses a NarX-based chimera capable of sensing vancomycin. For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertVanS-NarX
UseCell free expressionTagsMycExpressionBacterialMutationTransmembrane and sensing domain replaced to dete…PromoterT7Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only