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We narrowed to 18,548 results for: Mut

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Showing: 3121 - 3140 of 18548 results

  1. AAV-EF1α1.1-FLPX-rc [ChrimsonR-tdTomato]

    Plasmid
    #128587
    Purpose
    AAV-mediated expression of ChrimsonR-tdTomato under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Insert
    ChrimsonR-tdTomato
    Use
    AAV
    Tags
    tdTomato (codon diversified version)
    Expression
    Mammalian
    Mutation
    Chrimson K176R mutant
    Promoter
    EF1α (1.1 kb short version)
    Available Since
    Sept. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    128587-plasmid-map-sequence-id-257676

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  4. PA-DAD-dark

    Plasmid
    #53096
    Purpose
    Expresses dark mutant PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light with LOV2 in the closed conformation
    Depositor
    Klaus Hahn, Ronen Zaidel-Bar
    Article
    Rao et al Cytoskeleton (Hoboken). 2013 Jul;70(7):3
    Insert
    LOV(dark)-6aa-DAD
    Tags
    6x His and mVenus
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    L47F and C49S in Venus; C450M mutation in LOV2
    Available Since
    Aug. 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    53096-plasmid-map-sequence-id-119521

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  5. pαH-S-GSAS-B.1.1.7

    Plasmid
    #171747
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-B.1.1.7 variant (UK)
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike (S-GSAS-B.1.1.7 variant) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available Since
    July 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    171747-plasmid-map-sequence-id-337765

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  7. pAAV-EF1α1.1-FLEX-rc [ChrimsonR-GFP]

    Plasmid
    #108273
    Purpose
    AAV-mediated expression of ChrimsonR-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Insert
    ChrimsonR-GFP
    Use
    AAV
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Chrimson K176R mutant
    Promoter
    EF1α promoter (1.1kb short version)
    Available Since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    108273-plasmid-map-sequence-id-213551

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  10. FLAG-FullLength-TET1-CXXC/LLAA

    Plasmid
    #124399
    Purpose
    Full-length mutant TET1 (L897A/L900A), missing zinc finger domain (585-624), used to PiggyBAC rescue the function of TET1/2 DKO cells
    Depositor
    Shaun Cowley
    Article
    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.
    Insert
    FLAG-FullLength-TET1 CXXC zinc finger deletion (585-624) and L897A/L900A mutations (TET1 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    CXXC zinc finger deletion (aa 585-624) and L897A/…
    Available Since
    May 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    124399-plasmid-map-sequence-id-245260

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  15. pMIG-hNFATc2/C(RIT)-FLAG-AVITEV

    Plasmid
    #74053
    Purpose
    retroviral expression plasmid for human NFATc2/C (with RIT mutation abrogating AP1 binding) with C-terminal BirA-biotinylation signal and TEV celavage site
    Depositor
    Ria Baumgrass
    Article
    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.
    Insert
    human NFATc2, isoform C (NFATC2 Human)
    Use
    Retroviral
    Tags
    AVI-TEV and FLAG
    Expression
    Mammalian
    Mutation
    silent mutation A1723C in the sequence of NM_1730…
    Promoter
    pMSCV-LTRs
    Available Since
    June 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    74053-plasmid-map-sequence-id-142273

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  16. pIS1 mIL6R

    Plasmid
    #60797
    Purpose
    Luciferase reporter to measure miR-155 mediated differential repression. Contains IL6R 3' UTR and mutated miR-155 sites
    Depositor
    David Bartel
    Article
    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi
    Insert
    IL6R 3'UTR and mutated miR-155 binding site (IL6R Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    mutated miR-155 binding site
    Promoter
    thymidine kinase (HSV-TK promoter)
    Available Since
    Oct. 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    60797-plasmid-map-sequence-id-97808

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  19. pαH-S-GSAS-D614G-deltaFV

    Plasmid
    #171743
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein mink variant (S-GSAS-D614G-deltaFV variant)
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike (S-GSAS-D614G-deltaFV variant) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available Since
    July 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    171743-plasmid-map-sequence-id-337714

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Showing: 3121 - 3140 of 18548 results