We narrowed to 7,127 results for: trac

1. pQCXIZ GFP PODXL A WT

   Plasmid

   #202413

   Purpose

   Expression of GFP-tagged PODXL (isoform A) WT

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

   Insert

   PODXL (PODXL Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-hARΔ142–337

   Plasmid

   #89106

   Purpose

   Mammalian expression of human androgen receptor with deletion of amino acids 142-337 N-terminal transactivation function 1

   Depositor

   Elizabeth Wilson

   Article

   Titus et al PLoS One. 2012;7(1):e30192. doi: 10.1371

   Insert

   hAR delta 142-337 (AR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   hAR delta 142-337

   Promoter

   CMV

   Available since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

3. pUb FLAG-Human IntS6

   Plasmid

   #198408

   Purpose

   Expresses FLAG-tagged human IntS6

   Depositor

   Jeremy Wilusz

   Article

   Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

   Insert

   DDX26 (INTS6 Human)
   

   Use

   Tags

   3x FLAG

   Expression

   Insect

   Mutation

   Promoter

   Ubi-p63e

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-FRT-stop-FRT(FSF)- loxP-stop-loxP(LSL)-SP-HA-HRPtm-WPRE-bGHpolyA

   Plasmid

   #197044

   Purpose

   In situ cell-surface proteome extraction by extracellular labeling (iPEEL)

   Depositor

   Liqun Luo

   Article

   Shuster et al Neuron. 2022 Dec 7;110(23):3882-3896.e9.

   Inserts

   pCAG-FSF-LSL
   SP-HA-HRPtm
   WPRE-bGHpolyA
   

   Use

   Tags

   HA, myc

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO-Tet-On-shRNA-Control

   Plasmid

   #98398

   Purpose

   Lentivirus for expression of non-targeting control shRNA (Dox-inducible)

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   non-targeting randomized sequence
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDH-EF1-E-NoMi-P2A-CopGFP-T2A-PuroR

   Plasmid

   #207805

   Purpose

   E-NoMi is a re-engineered tetraspanin scaffold tagged with bioluminescent and fluorescent reporter proteins under an EF-1α promoter.

   Depositor

   Koen Breyne

   Article

   Maalouf et al Mol Ther. 2023 Jul 5;31(7):2206-2219. do

   Insert

   E-NoMi
   

   Use

   Lentiviral

   Tags

   3xFlag tag, copGFP, mCherry, and nanoluciferase

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1alpha

   Available since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCEP4-myc-ACE2

   Plasmid

   #141185

   Purpose

   Mammalian expression plasmid for human ACE2 with an N-terminal (extracellular) c-myc tag

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. PX459v3

   Plasmid

   #178799

   Purpose

   SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh (Cas9-2A Puro) and U6 (gRNA)

   Available since

   Aug. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-GGamma2-GFP2

   Plasmid

   #166774

   Purpose

   Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma2-GFP2 (GNG2 Synthetic, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains an N-terminal GFP2 and GSAG linker

   Promoter

   CMV

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1-GGamma9-GFP2

    Plasmid

    #140991

    Purpose

    Encodes a G gamma subunit (GNG9) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma9-GFP2 (GNGT2 Human)
    

    Use

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1 HA CFP hNKCC1 WT (NT15-H)

    Plasmid

    #49077

    Purpose

    Expresses human NKCC1 with an N-terminal 3xHA-CFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Use

    Tags

    3x HA and mCerulean

    Expression

    Mammalian

    Mutation

    262 silent mutations from native hNKCC1

    Promoter

    CMV

    Available since

    Nov. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. tetO-ESRRA

    Plasmid

    #170683

    Purpose

    doxycycline-inducible overexpression of ESRRA

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    ESRRA (ESRRA Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLIK-IDH2-R172K-FLAG

    Plasmid

    #66807

    Purpose

    Tet-inducible expression of mutant IDH2 in mammalian cells

    Depositor

    Christian Metallo

    Article

    Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

    Insert

    IDH2 (IDH2 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    R172K

    Promoter

    CMV

    Available since

    Feb. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. EGFPC2-Gephyrin P1

    Plasmid

    #68815

    Purpose

    expression of fluorescent tagged Geph in mammalian cells

    Depositor

    Shiva Tyagarajan

    Article

    Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

    Insert

    Gephyrin (Gphn Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    silent mutations on internal EcoRI site. Gephyri…

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHLS-EF1a-FKBP12-Gag(HIV)

    Plasmid

    #138476

    Purpose

    Expresses FKBP12 fused Gag for packaging FRB-SpCas9 into NanoMEDIC particle.

    Depositor

    Akitsu Hotta

    Article

    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

    Insert

    HIV Gag
    

    Use

    Tags

    FRBP12 and Lynn Myristolation site

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-GGamma12-GFP2

    Plasmid

    #166781

    Purpose

    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma12-GFP2 (GNG12 Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains an N-terminal GFP2 and GSAG linker

    Promoter

    CMV

    Available since

    April 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1-GGamma3-GFP2

    Plasmid

    #166775

    Purpose

    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma3-GFP2 (GNG3 Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains an N-terminal GFP2 and GSAG linker

    Promoter

    CMV

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCSIIpuro-BTC-ScNeo

    Plasmid

    #209898

    Purpose

    To monitor the status of Betacellulin, the plasmid encodes a recombinant BTC fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    BTC-ScNeo (BTC Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen and mScarlet

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCSIIpuro-NRG1-ScNeo

    Plasmid

    #209900

    Purpose

    To monitor the status of NRG1, the plasmid encodes a recombinant NRG1 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    NRG1-ScNeo (NRG1 Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen and mScarlet

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-GGamma7-GFP2

    Plasmid

    #166778

    Purpose

    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma7-GFP2 (GNG7 Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains an N-terminal GFP2 and GSAG linker

    Promoter

    CMV

    Available since

    April 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge