We narrowed to 9,413 results for: try

1. LentiGuide-Puro-GFPg1 (BB09)

   Plasmid

   #139461

   Purpose

   Lentiviral vector with gRNA targeting GFP; includes puromycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   GFP-targeting sgRNA inserted; resistance gene: puroR
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a / U6

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. FLAG-dCas13b-ADAR2DD

   Plasmid

   #190913

   Purpose

   Addgene #103871 with 3x FLAG on N-terminal of dCas13

   Depositor

   Timothy Jarome

   Article

   Farrell et al Mol Psychiatry. 2023 May 17. doi: 10.103

   Insert

   3X FLAG
   

   Use

   CRISPR

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ230-RVQ

   Plasmid

   #200187

   Purpose

   Gateway entry clone (attL1 & attR5) for LbCas12a-RVQ mediated genome editing

   Depositor

   Yiping Qi

   Article

   Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

   Insert

   LbCas12a-RVQ
   

   Use

   CRISPR; Gateway compatible lbcas12a-rv entry clone

   Tags

   NLS

   Expression

   Plant

   Mutation

   G146R, R182V, E795Q

   Promoter

   Available since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMuLE ENTR 7SK-miR-30 L1-L4

   Plasmid

   #62180

   Purpose

   MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Type

   Empty backbone

   Use

   RNAi; Mule gateway entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pMuLE ENTR CMV Luc2 (FF-Luciferase) L5-L2

   Plasmid

   #62168

   Purpose

   MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and firefly luciferase module. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Insert

   Luciferase
   

   Use

   Luciferase; Mule gateway entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR1A DHFR.cHSF1

   Plasmid

   #134737

   Purpose

   Gateway entry vector for constitutively active HSF1 fused to the DHFR destabilized domain

   Depositor

   Matthew D. Shoulders

   Article

   Phillips et al Elife. 2017 Sep 26;6. doi: 10.7554/eLife

   Insert

   DHFR.cHSF1 (HSF1 Human)
   

   Use

   Entry vector for gateway cloning

   Tags

   DHFR

   Expression

   Mutation

   Deletion of amino acids 186-202

   Promoter

   None

   Available since

   Oct. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. KCC2B_HUMAN_D0

   Plasmid

   #79718

   Purpose

   This plasmid encodes the kinase domain of KCC2B. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Markus Seeliger, Nicholas Levinson

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   KCC2B
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pPB-hCMV1-hNANOG-IV

   Plasmid

   #60432

   Purpose

   tet-inducible human NANOG

   Depositor

   Austin Smith

   Article

   Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

   Insert

   NANOG (NANOG Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. CBP TAZ1(340-439)

   Plasmid

   #173760

   Purpose

   bacterial expression of mouse CBP TAZ1 domain

   Depositor

   Peter Wright

   Article

   De Guzman et al Biochemistry. 2005 Jan 18;44(2):490-7. d

   Insert

   CBP TAZ1 (Crebbp Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ266

    Plasmid

    #164713

    Purpose

    Gateway entry clone (attL1 & attR5) PmCDA1-CBE_V01 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    zCas9(D10A)-PmCDA1-UGI
    

    Use

    CRISPR; Gateway compatible pmcda1-cbe_v01 entry c…

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. LentiGuide-Neo-CTRLg2

    Plasmid

    #139451

    Purpose

    Lentiviral vector with non-targeting gRNA and neomycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    Non-targeting sgRNA inserted; resistance gene: neoR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a / U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-CALCOCO1

    Plasmid

    #137765

    Purpose

    Expression and localization of tagged CALCOCO1

    Depositor

    Carol Mercer

    Article

    Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

    Insert

    CALCOCO1 (CALCOCO1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. SERPINA-bio-His

    Plasmid

    #52182

    Purpose

    Expresses full-length Alpha-1-antitrypsin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    SERPINA (SERPINA1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. LentiGuide-Puro-CTRLg1 (CG509)

    Plasmid

    #139455

    Purpose

    Lentiviral vector with non-targeting gRNA and puromycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    Non-targeting sgRNA inserted; resistance gene: puroR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a / U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a-DONSON

    Plasmid

    #208797

    Purpose

    Xenopus laevis His6-Myc-DONSON wild type

    Depositor

    Aga Gambus

    Article

    Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

    Insert

    DONSON (donson.L Frog)
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. MK14_HUMAN_D0

    Plasmid

    #79680

    Purpose

    This plasmid encodes the kinase domain of MK14. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Markus Seeliger, Nicholas Levinson

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    MK14
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pET51b-His-TEV-HOB_(HaloTag7_oligo_binder)

    Plasmid

    #167268

    Purpose

    Overexpression of the HaloTag7 variant HOB (halo-based oligonucleotide binder) in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    HOB
    

    Use

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. IG510: pMVP (R4-R3) human PDX1

    Plasmid

    #121744

    Purpose

    pMVP R4-R3 entry plasmid, contains human PDX1 for 3- or 4-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Human PDX1 (PDX1 Human)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYPQ166-iSpyMac

    Plasmid

    #149553

    Purpose

    Gateway entry clone for CRISPR-iSpyMacCas9 genome editing at NAAR PAMs

    Depositor

    Yiping Qi

    Article

    Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

    Insert

    iSpyMacCas9
    

    Use

    CRISPR; Gateway compatible zispymaccas9 entry clo…

    Tags

    3X FLAG, NLS and NLS

    Expression

    Plant

    Mutation

    R221K, N394K, Protospacer interacting domain form…

    Promoter

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNSSCPG

    Plasmid

    #183187

    Purpose

    PACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; sfGFP-LAA expressed under T7 p

    Depositor

    Hsiao-Chun Huang

    Article

    Lin et al Nat Commun. 2021 Feb 9;12(1):888. doi: 1

    Inserts

    superfolder GFP
    SpmX450 and T7 181+ fusion protein
    T7 RNAP 1-179 variant and SpmX450 fusion protein
    mRFP1 and popZ fusion protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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