We narrowed to 5,876 results for: nls

1. U6_sgRNA_CAG_ABE7.10_St1Cas9_LMD9

   Plasmid

   #136660

   Purpose

   Expresses ABE St1Cas9 LMD9 in mammalian cells along with its U6-driven sgRNA

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Inserts

   St1Cas9 LMD-9
   sgRNA for St1Cas9
   ABE7.10
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40 NLS and hSt1Cas9

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJ4M/TDP-43 S48E

   Plasmid

   #104481

   Purpose

   bacterial expression of TDP-43 S48E

   Depositor

   Nicolas Fawzi

   Article

   Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   MBP-6xHis

   Expression

   Bacterial

   Mutation

   S48E

   Promoter

   Available since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. TDP-43_NTD1-80_Y4R

   Plasmid

   #104478

   Purpose

   bacterial expression of TDP-43 NTD Y4R

   Depositor

   Nicolas Fawzi

   Article

   Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Y4R

   Promoter

   Available since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. RP1B FUS 1-163 QQ4xSS #2

   Plasmid

   #127195

   Purpose

   Protein expression for affinity purification

   Depositor

   Nicolas Fawzi

   Article

   Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-

   Insert

   FUS 1-163 QQ4xSS #2 (FUS Human)
   

   Use

   Tags

   hexahistidine

   Expression

   Bacterial

   Mutation

   QQ4xSS #2:Q8S, Q9S, Q60S, Q62S, Q102, Q103, Q139S…

   Promoter

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. TDP-43_NTD1-80_E17R

   Plasmid

   #104479

   Purpose

   bacterial expression of TDP-43 NTD E17R

   Depositor

   Nicolas Fawzi

   Article

   Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   E17R

   Promoter

   Available since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCW-ND-Cdt1-HA-Puro

   Plasmid

   #193767

   Purpose

   Doxycyline-inducible (TetOn) human Cdt1 (SV40 NLS localization and C-terminal HA tag) with mutations which abroggate its S phase degradation, expressed from all-in-one pCW vector (TetOn + rtTA)

   Depositor

   Tobias Meyer

   Article

   Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

   Insert

   ND-Cdt1-HA (CDT1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…

   Promoter

   TRE

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. BE3(E63Q)-P2A-EGFP (pRZ189)

   Plasmid

   #123613

   Purpose

   CAG promoter expression plasmid for rAPOBEC1(E63Q)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (catalytically impaired BE3 mutant).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

   Insert

   BE3(E63Q)-P2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E63Q in rAPOBEC1, D10A in SpCas9

   Promoter

   CAG

   Available since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTE3328

   Plasmid

   #107539

   Purpose

   Expresses As crRNA and human codon optimized AsCpf1(RR mutant) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   As crRNA
   hAsCpf1(RR mutant)
   

   Use

   Tags

   3xHA, NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   S542R, K607R

   Promoter

   CMV and human U6

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTE3329

   Plasmid

   #107538

   Purpose

   Expresses Lb crRNA and human codon optimized LbCpf1(RR mutant) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   Lb crRNA
   hLbCpf1(RR mutant)
   

   Use

   Tags

   3xHA, NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   G532R, K595R

   Promoter

   CMV and human U6

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTE3327

    Plasmid

    #107535

    Purpose

    Expresses As crRNA and human codon optimized AsCpf1(RVR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    As crRNA
    hAsCpf1(RVR mutant)
    

    Use

    Tags

    3xHA, NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    S542R, K548V, N552R

    Promoter

    CMV and human U6

    Available since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. DNMT3ACD-CRY2-EGFP

    Plasmid

    #82556

    Purpose

    Encodes DMNT3 under LITE2.0 system

    Depositor

    Joseph Irudayaraj

    Article

    Lo et al Sci Rep. 2017 Feb 9;7:42047. doi: 10.103

    Inserts

    DNMT3A (DNMT3A Human)
    CRY2 (CRY2 Mustard Weed)
    EGFP
    

    Use

    AAV

    Tags

    2A, NLS(alpha-imp), and NLS-VP64

    Expression

    Mammalian

    Mutation

    CRY2PHR (N-terminal photolyase homology region do…

    Promoter

    EF-1alpha

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. hnRNPA1_allW_D262V-FLAG-IRES-eGFP

    Plasmid

    #234621

    Purpose

    Mammalian expression of full-length hnRNPA1 D262V with all aromatic amino acids in the C-terminal LCD mutated to W keeping the hexapeptide fibril core (SYNDFG) and the PY-NLS intact.

    Depositor

    Tanja Mittag

    Article

    Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

    Insert

    hnRNPA1 allW D262V (HNRNPA1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Famillial ALS mutation D262V, all aromatic amino …

    Promoter

    CMV

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. TDP-43 _CTD_5A→M_IDR + 5M→A_CR

    Plasmid

    #194268

    Purpose

    Expresses 6xHis-tagged C-terminal domain of TDP-43 with TDP-43 _CTD_5A→M_IDR + 5M→A_CR

    Depositor

    Nicolas Fawzi

    Article

    Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

    Insert

    TDP-43 (TARDBP Synthetic, Human)
    

    Use

    Tags

    6XHistag

    Expression

    Bacterial

    Mutation

    A297M, A367M, A381M, A388M, A391M M322A, M323A, M…

    Promoter

    T7

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGMC00003

    Plasmid

    #166720

    Purpose

    sgRNA against mouse Kitl

    Depositor

    Raj Chari

    Article

    Rice et al Nat Commun. 2018 Nov 30;9(1):5099. doi:

    Insert

    Kitl (Kitl Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGMC00004

    Plasmid

    #166721

    Purpose

    sgRNA against mouse Kitl

    Depositor

    Raj Chari

    Article

    Rice et al Nat Commun. 2018 Nov 30;9(1):5099. doi:

    Insert

    Kitl (Kitl Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. MBP-hnRNPA2_LC_R191K_R201K_R216K_R254K

    Plasmid

    #104467

    Purpose

    express MBP hnRNPA2 LC with 4 R to K mutations

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    R191K R201K R216K R254K

    Promoter

    Available since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. SGEP-sh-Hs-PHGDH-1956

    Plasmid

    #188670

    Purpose

    shRNA

    Depositor

    Lewis Cantley

    Insert

    shPHGDH (PHGDH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. SGEP-sh-Hs-PHGDH-1883

    Plasmid

    #188669

    Purpose

    shRNA

    Depositor

    Lewis Cantley

    Insert

    shPHGDH (PHGDH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. TC9pAB

    Plasmid

    #71310

    Purpose

    used as a template for in vitro transcription of Cas9 mRNA after linearizing with BbsI

    Depositor

    Karl Degenhardt

    Insert

    Cas9
    

    Use

    In vitro transcription

    Tags

    NLS

    Expression

    Mutation

    Promoter

    T7

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. PX381 Filifactor alocis Cas9

    Plasmid

    #68318

    Purpose

    Filifactor alocis Cas9

    Depositor

    Feng Zhang

    Insert

    FaCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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