We narrowed to 17,163 results for: Emb;
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Plasmid#155361PurposeGentamycin version of the scarless genome engineering pDEL plasmid (Tikh et al. 2016) compatible with SalmonellaDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceSept. 30, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits
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pOD_003
Plasmid#155362PurposeZeomycin version of the scarless genome engineering pDEL plasmid (Tikh et al. 2016) compatible with SalmonellaDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceSept. 30, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDEST-pSP172BSSPE-pFOGc::RfA
Plasmid#186397PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned gene of interest under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::RfA
Plasmid#186396PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned gene of interest under control of a regulatory sequence cloned in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc-sgRNA-Cas9.scr_1
Plasmid#190703PurposeExpresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cellsDepositorInsertScrambled sgRNA 1
UseCRISPRExpressionInsectPromoterDrosophila U6Available SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc-sgRNA-Cas9.scr_3
Plasmid#190705PurposeExpresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cellsDepositorInsertScrambled sgRNA 3
UseCRISPRExpressionInsectPromoterDrosophila U6Available SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-L3-a-element-L4
Plasmid#186366PurposeEntry clone with ORF encoding Otx cis-regulatory region flanked by Gateway recombination sequencesDepositorInserta-element early Otx neural enhancer
UseProtein expressionPromoterT7 promoterAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-L1-Kozak-Venus-stop-L2
Plasmid#186360PurposeEntry clone with ORF encoding Venus fluorescent protein flanked by Gateway recombination sequencesDepositorInsertVenus yellow fluorescent protein
UseExpression of the venus fluorescent proteinAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB556
Plasmid#185108Purpose1st 200 amino acids (N-term + transmembrane domain) of Pom152 fused to HA and GFP with S158A, F159A, F160ADepositorInsertPOM152
TagsGFPExpressionYeastMutation1st 200 amino acids (N-term + transmembrane domai…Available SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB553
Plasmid#185107Purpose1st 200 amino acids (N-term + transmembrane domain) of Pom152 fused to HA and GFP with Y180L, Q182LDepositorInsertPOM152
TagsGFPExpressionYeastMutation1st 200 amino acids (N-term + transmembrane domai…Available SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
PDEST-pSP172BSSPE-pFOGc::RfA-HA
Plasmid#186406PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter HA tag under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-HA-DmDCP1_1-327-dsRNAres_H
Plasmid#146501PurposeInsect Expression of DmDCP1_1-327DepositorInsertDmDCP1_1-327 (Dcp1 Fly)
ExpressionInsectAvailable SinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-HA-DmDCP1-dsRNAres_H
Plasmid#146502PurposeInsect Expression of DmDCP1DepositorInsertDmDCP1 (Dcp1 Fly)
ExpressionInsectAvailable SinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-DmDCP1_328-366 (MCSII)_G
Plasmid#146402PurposeBacterial Expression of DmDCP1_328-366DepositorInsertDmDCP1_328-366 (Dcp1 Fly)
ExpressionBacterialAvailable SinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmDCP1_327-372_H
Plasmid#146500PurposeInsect Expression of DmDCP1_327-372DepositorInsertDmDCP1_327-372 (Dcp1 Fly)
ExpressionInsectAvailable SinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
ppwd1.473.646.W601A
Plasmid#137660PurposeExpresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.DepositorAvailable SinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-cPla2-C2-D34R-D87R-EGFP-6H
Plasmid#187188PurposeProtein ExpressionDepositorAvailable SinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-cPla2-C2-L32F-L33F-EGFP-6H
Plasmid#187184PurposeProtein ExpressionDepositorAvailable SinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-cPla2-C2-Y90A-V91A-EGFP-6H
Plasmid#187185PurposeProtein ExpressionDepositorAvailable SinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-cPla2-C2-L32A-L33A-EGFP-6H
Plasmid#187183PurposeProtein ExpressionDepositorAvailable SinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-Alox12-PLAT-EGFP-6H
Plasmid#187190PurposeProtein ExpressionDepositorAvailable SinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-hs-PKCgamma-C2-EGFP-6H
Plasmid#187115PurposeProtein ExpressionDepositorAvailable SinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-cPla2-C2-D34K-D87K-EGFP-6H
Plasmid#187187PurposeProtein ExpressionDepositorAvailable SinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458_sgRNA_Ago1_5
Plasmid#172470PurposeExpresses SpCas9, GFP, and sgRNA targeting Ago1DepositorInsertsgAgo1
UseCRISPRExpressionMammalianPromoterhU6Available SinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458_sgRNA_Ago1_6
Plasmid#172471PurposeExpresses SpCas9, GFP, and sgRNA targeting Ago1.DepositorInsertsgAgo1
UseCRISPRExpressionMammalianPromoterhU6Available SinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
IBAR-Cry2PHR-WH2
Plasmid#176605PurposeCry2PHR with N-terminal IBAR domain and C-terminal WH2 domainDepositorInsertIBAR-Cry2PHR-mCherry
ExpressionMammalianPromoterCMVAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cry2PHR-IBAR-WH2
Plasmid#176603PurposeCry2PHR with C-terminal IBAR and WH2 domainsDepositorInsertCry2PHR-mCherry-IBAR-WH2
ExpressionMammalianPromoterCMVAvailable SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTK73_htz-1
Plasmid#174546Purposehtz-1 targeting gRNA expressionDepositorInserthtz-1 targeting gRNA
ExpressionWormPromoterCe-U6 promoterAvailable SinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-WIPI2dR108E-cs-TEV-STREP
Plasmid#171835PurposeMammalian expression of human WIPI2d R108E with C-terminal StrepDepositorAvailable SinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-WIPI2dR125E-cs-TEV-STREP
Plasmid#171836PurposeMammalian expression of human WIPI2d R125E with C-terminal StrepDepositorAvailable SinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-WIPI2dK128E-cs-TEV-STREP
Plasmid#171837PurposeMammalian expression of human WIPI2d K128E with C-terminal StrepDepositorAvailable SinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-WIPI2dH85E-cs-TEV-STREP
Plasmid#171831PurposeMammalian expression of human WIPI2d H85E with C-terminal StrepDepositorAvailable SinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-WIPI2dK88E-cs-TEV-STREP
Plasmid#171832PurposeMammalian expression of human WIPI2d K88E with C-terminal StrepDepositorAvailable SinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-WIPI2dI92E-cs-TEV-STREP
Plasmid#171833PurposeMammalian expression of human WIPI2d I92E with C-terminal StrepDepositorAvailable SinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-WIPI2dC93E-cs-TEV-STREP
Plasmid#171834PurposeMammalian expression of human WIPI2d C93E with C-terminal StrepDepositorAvailable SinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN086
Plasmid#173448Purposeexpress E. coli E69 (O9a:K30) Wzc_K540M with Y715EDepositorInsertE. coli E69 (O9a:K30) Wzc_K540M with Y715E
Tagshexa-histidine tagExpressionBacterialMutationK540M, Y715EPromoteraraBADAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN087
Plasmid#173449Purposeexpress E. coli E69 (O9a:K30) Wzc_K540M with Y717EDepositorInsertE. coli E69 (O9a:K30) Wzc_K540M with Y717E
Tagshexa-histidine tagExpressionBacterialMutationK540M, Y717EPromoteraraBADAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN088
Plasmid#173450Purposeexpress E. coli E69 (O9a:K30) Wzc_K540M with Y718EDepositorInsertE. coli E69 (O9a:K30) Wzc_K540M with Y718E
Tagshexa-histidine tagExpressionBacterialMutationK540M, Y718EPromoteraraBADAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN089
Plasmid#173451Purposeexpress E. coli E69 (O9a:K30) Wzc_K540M with Y717E, Y718EDepositorInsertE. coli E69 (O9a:K30) Wzc_K540M with Y717E, Y718E
Tagshexa-histidine tagExpressionBacterialMutationK540M, Y717E, Y718EPromoteraraBADAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN095
Plasmid#173765Purposeexpress E. coli E69 (O9a:K30) Wzc with periplasmic motif 3 truncationDepositorInsertE. coli E69 (O9a:K30) Wzc with periplasmic motif 3 truncation
Tagshexa-histidine tagExpressionBacterialMutationdelete motif 3PromoteraraBADAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN081
Plasmid#173443Purposeexpress E. coli E69 (O9a:K30) Wzc_K540M with Y705EDepositorInsertE. coli E69 (O9a:K30) Wzc_K540M with Y705E
Tagshexa-histidine tagExpressionBacterialMutationK540M, Y705EPromoteraraBADAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN082
Plasmid#173444Purposeexpress E. coli E69 (O9a:K30) Wzc_K540M with Y706EDepositorInsertE. coli E69 (O9a:K30) Wzc_K540M with Y706E
Tagshexa-histidine tagExpressionBacterialMutationK540M, Y706EPromoteraraBADAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN083
Plasmid#173445Purposeexpress E. coli E69 (O9a:K30) Wzc_K540M with Y705E, Y706EDepositorInsertE. coli E69 (O9a:K30) Wzc_K540M with Y705E, Y706E
Tagshexa-histidine tagExpressionBacterialMutationK540M, Y705E, Y706EPromoteraraBADAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN084
Plasmid#173446Purposeexpress E. coli E69 (O9a:K30) Wzc_K540M with Y708EDepositorInsertE. coli E69 (O9a:K30) Wzc_K540M with Y708E
Tagshexa-histidine tagExpressionBacterialMutationK540M, Y708EPromoteraraBADAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN085
Plasmid#173447Purposeexpress E. coli E69 (O9a:K30) Wzc_K540M with Y713EDepositorInsertE. coli E69 (O9a:K30) Wzc_K540M with Y713E
Tagshexa-histidine tagExpressionBacterialMutationK540M, Y713EPromoteraraBADAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN080
Plasmid#167961Purposeexpress E. coli E69 (O9a:K30) Wzc with Y717E, Y718EDepositorInsertE. coli E69 (O9a:K30) Wzc with Y717E, Y718E
Tagshexa-histidine tagExpressionBacterialMutationY717E, Y718EPromoteraraBADAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN215
Plasmid#167962Purposeexpress E. coli E69 (O9a:K30) Wzc with Y715E, Y717E, Y718EDepositorInsertE. coli E69 (O9a:K30) Wzc with Y715E, Y717E, Y718E
Tagshexa-histidine tagExpressionBacterialMutationY715E, Y717E, Y718EPromoteraraBADAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN261
Plasmid#167966Purposeexpress E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717EDepositorInsertE. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E
Tagshexa-histidine tagExpressionBacterialMutationY713E, Y715E, Y717EPromoteraraBADAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN008
Plasmid#167949Purposeexpress E. coli E69 (O9a:K30) Wzc with K540M mutationDepositorInsertbacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30), K540M nutant
Tagshexa-histidine tagExpressionBacterialMutationK540MPromoteraraBADAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN093
Plasmid#167951Purposeexpress E. coli E69 (O9a:K30) Wzc_K540M with periplasmic motif 3 truncationDepositorInsertE. coli E69 (O9a:K30) Wzc_K540M with periplasmic motif 3 truncation
Tagshexa-histidine tagExpressionBacterialMutationK540M, delete motif 3PromoteraraBADAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only