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pME-myristo

Plasmid

#109541

Purpose

Multisite gateway vector for 5' tagging with the 5' myristoylation sequence from ARF5

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

5' myristoylation sequence from ARF5

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pXN-FBLWLF-GAL80

Plasmid

#26265

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

GAL80 (GAL80 Budding Yeast)

Use

Drosophila transgenesis

Expression

Bacterial and Insect

Available Since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pML1

Plasmid

#108332

Purpose

MIDAS Level-1 source vector for cloning of modules (synthetic biology parts)

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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XW21

Plasmid

#65837

Purpose

XW21 Punc-4c-QS-3'UTR-unc-54 (no SL2-mcherry)

Depositor

Kang Shen

Article

Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

Insert

Neurospora crassa OR74A repressor (qa-1S)

Expression

Worm

Promoter

Punc-4c

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pKFSS1_Psyn-sgRNA500

Plasmid

#149557

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

Psyn and pSyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2_Psyn-mCherryBb

Plasmid

#149636

Purpose

Psyn-mCherry promoter strength reporter for use in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

Psyn-mCherryBb

Tags

mCherry

Expression

Bacterial

Promoter

Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-Dnm2a

Plasmid

#109571

Purpose

Multisite gateway vector for 3' tagging with Dynamin2a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Dynamin2a

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ1666

Plasmid

#51485

Purpose

miniMos transposable element with PuroR, MCS cloning

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Type

Empty backbone

Expression

Worm

Available Since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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pXN-FBLWLF-VP16AD

Plasmid

#26268

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

VP16AD-ZIP+

Use

Drosophila transgenesis

Expression

Bacterial and Insect

Available Since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pML3

Plasmid

#108341

Purpose

MIDAS Level-3 destination vector. Used for initiating Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV CB6 FFLuc

Plasmid

#35655

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Type

Empty backbone

Use

AAV

Available Since

July 11, 2012

Availability

Academic Institutions and Nonprofits only

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pXN-FBLWLF-GAL4AD

Plasmid

#26263

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

Gal4AD-Zip+ (GAL4 Budding Yeast)

Use

Drosophila transgenesis

Expression

Bacterial and Insect

Available Since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pEM352

Plasmid

#248764

Purpose

160xTetO Sponge Plasmid with BsaI sites flanking sponge casette for Golden Gate Assembly

Depositor

Carolina Tropini

Article

McCallum et al Cell. 2026 Feb 19;189(4):1245-1261.e21.

Insert

T7Te-rrnBT1-160xTetO

Expression

Bacterial

Available Since

March 25, 2026

Availability

Academic Institutions and Nonprofits only

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pRS316 Gal1p1

Plasmid

#242951

Purpose

Backbone plasmid for subcloning

Depositor

François-Xavier Campbell-Valois

Article

Bajunaid et al mBio. 2026 Mar 17:e0389025. doi: 10.1128

Insert

Gal_promoter-cycE terminator

Tags

Myc-tag

Expression

Yeast

Available Since

March 17, 2026

Availability

Academic Institutions and Nonprofits only

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pRS316 Gal1p1 IpgA

Plasmid

#242952

Purpose

Expression of IpgA in yeast cells

Depositor

François-Xavier Campbell-Valois

Article

Bajunaid et al mBio. 2026 Mar 17:e0389025. doi: 10.1128

Insert

IpgA (IcsB Chaperone)

Tags

Myc

Expression

Yeast

Available Since

March 17, 2026

Availability

Academic Institutions and Nonprofits only

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pRS313 Gal1p2

Plasmid

#242948

Purpose

Backbone plasmid for IcsB and homologs subcloning

Depositor

François-Xavier Campbell-Valois

Article

Bajunaid et al mBio. 2026 Mar 17:e0389025. doi: 10.1128

Insert

Backbone plasmid for subcloning

Tags

3X FLAG tag

Expression

Yeast

Available Since

March 17, 2026

Availability

Academic Institutions and Nonprofits only

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pRS313 Gal1p2 IcsB

Plasmid

#242949

Purpose

Expression of IcsB in yeast

Depositor

François-Xavier Campbell-Valois

Article

Bajunaid et al mBio. 2026 Mar 17:e0389025. doi: 10.1128

Insert

IcsB

Tags

3X FLAG tag

Expression

Yeast

Available Since

March 17, 2026

Availability

Academic Institutions and Nonprofits only

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pRS313 Gal1p2 H8

Plasmid

#242950

Purpose

Expression of homolog H8 in yest cells

Depositor

François-Xavier Campbell-Valois

Article

Bajunaid et al mBio. 2026 Mar 17:e0389025. doi: 10.1128

Insert

IcsB Homolog H8

Tags

3X FLAG tag

Expression

Yeast

Available Since

March 17, 2026

Availability

Academic Institutions and Nonprofits only

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pAH119

Plasmid

#248732

Purpose

PBT0479 biosensor circuit in pNBU2 integrative Bacteroides plasmid

Depositor

Carolina Tropini

Article

McCallum et al Cell. 2026 Feb 19;189(4):1245-1261.e21.

Insert

PBfP1E6-RBS1PLP-mScarletI3-rrnBT2-PBT0479-TetR-rrnBT2-pTetR56-RBS1PLP-mGL-rrnBT2

Expression

Bacterial

Available Since

March 16, 2026

Availability

Academic Institutions and Nonprofits only

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pLV[Exp]-Hygro-cTnT-FUCCIplex

Plasmid

#240216

Purpose

Multiplexable FUCCI sensor (FUCCIplex). The cTnT promoter drives the expression of the fluorescent sensor.

Depositor

Francesco Pasqualini

Article

Di Sante et al bioRxiv 2024.12.19.629259

Insert

FUCCIplex

Use

Lentiviral

Expression

Mammalian

Promoter

cTnT

Available Since

Feb. 9, 2026

Availability

Academic Institutions and Nonprofits only

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