We narrowed to 3,600 results for: gla

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pLKO.1-scrCtnnd2.1-GFP

Plasmid

#209096

Purpose

GFP expressing scramble of shRNA targeting Ctnnd2 N terminus

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

scrCtnnd2.1

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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NmMetNI-WT

Plasmid

#172114

Purpose

Expression of MetN and MetI membrane proteins

Depositor

Douglas Rees

Article

Sharaf et al Elife. 2021 Aug 19;10. pii: 69742. doi:

Inserts

MetN
MetI

Tags

10x His Tag and Enterokinase

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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MtMscL delta49

Plasmid

#118342

Purpose

M. tuberculosis MscL (delta49) in pET19b

Depositor

Douglas Rees

Article

Herrera et al Sci Rep. 2018 Oct 1;8(1):14566. doi: 10.

Insert

MscL (mscL Mycobacterium tuberculosis)

Tags

6x His Tag and Thrombin Cleavage site

Expression

Bacterial

Mutation

Changed Glutamic Acid (Glu) 102 to Stop Codon

Promoter

T7 Promoter

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMAL-SsoPCNA3

Plasmid

#107069

Purpose

Expresses S. solfataricus PCNA3 fused to MBP in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

S. solfataricus PCNA3

Tags

6xHis-thrombin cleavage site and MBP

Expression

Bacterial

Promoter

tac promoter

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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CTL2 (M2225R)

Plasmid

#96952

Purpose

Expresses C-Terminal segment of C. elegans Piezo1 with M2225R mutation in E. coli

Depositor

Douglas Rees

Article

Kamajaya et al Structure. 2014 Oct 7;22(10):1520-7. doi

Insert

CTL2 (M222R) of PIEZO1

Tags

6x Histidine Tag

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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36INS2-pBAD_GB

Plasmid

#78677

Purpose

Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [G:36INS2-pBAD:B]

Depositor

Douglas Densmore

Article

Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

Insert

Insulated pBAD Promoter

Use

Synthetic Biology

Available Since

Aug. 4, 2016

Availability

Academic Institutions and Nonprofits only

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36INS2-pBAD_FB

Plasmid

#78676

Purpose

Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [F:36INS2-pBAD:B]

Depositor

Douglas Densmore

Article

Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

Insert

Insulated pBAD Promoter

Use

Synthetic Biology

Available Since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only

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36INS2-pBAD_EB

Plasmid

#78675

Purpose

Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [E:36INS2-pBAD:B]

Depositor

Douglas Densmore

Article

Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

Insert

Insulated pBAD Promoter

Use

Synthetic Biology

Available Since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only

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36INS2-pBAD_AB

Plasmid

#78674

Purpose

Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [A:36INS2-pBAD:B]

Depositor

Douglas Densmore

Article

Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

Insert

Insulated pBAD Promoter

Use

Synthetic Biology

Available Since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only

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36INS1-pBAD_AB

Plasmid

#78670

Purpose

Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected.Modified from BBa_I13453 to adjust spacing in MoClo system. [A:36INS1-pBAD:B]

Depositor

Douglas Densmore

Article

Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

Insert

Insulated pBAD Promoter

Use

Synthetic Biology

Available Since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only

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DVK_FG

Plasmid

#66070

Purpose

MoClo Destination Vector: (based on pSB1K3) [F:LacZa:G]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Type

Empty backbone

Use

Synthetic Biology; Destination vector

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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DVK_GH

Plasmid

#66071

Purpose

MoClo Destination Vector: (based on pSB1K3) [G:LacZa:H]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Type

Empty backbone

Use

Synthetic Biology; Destination vector

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pTH1-SaSrtA-KQ

Plasmid

#64982

Purpose

Expresses the E105K/E108Q mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59, E105K, E108Q

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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pAC-EHER

Plasmid

#53262

Purpose

Contains a 7 gene carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin diglucoside in Escherichia coli

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.

Insert

crtE, idi, crtX, crtY, crtI, crtB, crtZ

Use

Low copy number bacterial cloning vector

Promoter

endogenous promoters

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pMALcPP-MS2cp

Plasmid

#203351

Purpose

Expresses His/MBP-tagged MS2-CP V75E/A81G variant in bacteria

Depositor

Thomas Cech

Article

Hemphill et al Proc Natl Acad Sci U S A. 2023 Jun 27;12

Insert

MS2 coat protein

Tags

Hx6-MBP

Expression

Bacterial

Mutation

V75E and A81G, to prevent capsid assembly

Available Since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSNAPf-hYTHDF3

Plasmid

#155348

Purpose

Express full-length human YTHDF3 with a C-terminal SNAP-tag in mammalian cells

Depositor

Xiaowei Zhuang

Article

Fu et al Nat Chem Biol. 2020 May 25. pii: 10.1038

Insert

YTHDF3 (YTHDF3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shControl-EGFP

Plasmid

#227685

Purpose

Expresses EGFP and scramble of shRNA targeting Atg7

Depositor

Cagla Eroglu

Article

Wang et al eLife 2025 [Reviewed Preprint] 14:RP1075

Insert

scrambled shRNA control

Expression

Mammalian

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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MBP-TEVcs(ENLYFQ/G)-His6-uTEV2Δ(220-242)-R5

Plasmid

#135456

Purpose

Bacterial expression of uTEV2Δ protease

Depositor

Alice Ting

Article

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5

Tags

MBP, His6

Expression

Bacterial

Mutation

S219V mutation improves stability, T30A/S153N mut…

Promoter

tac promoter

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pSmac-GFP

Plasmid

#40881

Depositor

Douglas Green

Article

Tait et al Dev Cell. 2010 May 18;18(5):802-13.

Insert

Smac-GFP (DIABLO Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 9, 2012

Availability

Academic Institutions and Nonprofits only
M13-kmR

Plasmid

#206861

Purpose

M13KE from NEB modified with kanamycin resistance gene and NotI restriction site on g3p ( M13KE-NotI-Kan)

Depositor

Irene Chen

Article

Lin et al PLoS One. 2011;6(5):e19991. doi: 10.1371

Insert

Kanamycin resistance gene

Expression

Bacterial

Promoter

lac

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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