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CEA-N domain residues 1-132 (CEACAM5)-pET30b

Plasmid

#174693

Purpose

Plasmid expressing CEA-N domain

Depositor

Jean Gariepy

Article

Abdul-Wahid et al Int J Cancer. 2016 Aug 15;139(4):841-53.

Insert

CEA-N Domain

Use

Tags

TEV Cleavage Site and hexahistidine (HIS) tag

Expression

Bacterial

Mutation

none

Promoter

T7 Promoter

Available since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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AT5G63930 X059_pECIA2

Plasmid

#115116

Purpose

Bait vector AT5G63930 X059_pECIA2 should be used with prey vector AT5G63930 X059_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G63930 (AT5G63930 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT1G17230 X074_pECIA2

Plasmid

#115096

Purpose

Bait vector AT1G17230 X074_pECIA2 should be used with prey vector AT1G17230 X074_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G17230 (AT1G17230 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT2G33170 X049_pECIA2

Plasmid

#115101

Purpose

Bait vector AT2G33170 X049_pECIA2 should be used with prey vector AT2G33170 X049_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT2G33170 (AT2G33170 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT1G74360 X038_pECIA2

Plasmid

#115080

Purpose

Bait vector AT1G74360 X038_pECIA2 should be used with prey vector AT1G74360 X038_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G74360 (AT1G74360 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
AT4G23740 J11_pECIA2

Plasmid

#114998

Purpose

Bait vector AT4G23740 J11_pECIA2 should be used with prey vector AT4G23740 J11_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT4G23740 (AT4G23740 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.JARID2

Plasmid

#125166

Purpose

Expresses human JARID2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

JARID2 (JARID2 Human)

Use

Tags

His-MBP

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.EPOP

Plasmid

#125170

Purpose

Expresses human EPOP in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

EPOP (EPOP Human)

Use

Tags

His-MBP

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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RP1B FUS 1-163 QQ4xSS #1

Plasmid

#127194

Purpose

Protein expression for affinity purification

Depositor

Nicolas Fawzi

Article

Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-

Insert

FUS 1-163 QQ4xSS #1 (FUS Human)

Use

Tags

hexahistidine

Expression

Bacterial

Mutation

QQ4xSS #1: Q27S, Q28S, Q93S, Q94S, Q132S, Q133S, …

Promoter

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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AT5G62710 X045_pECIA2

Plasmid

#115130

Purpose

Bait vector AT5G62710 X045_pECIA2 should be used with prey vector AT5G62710 X045_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G62710 (AT5G62710 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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RP1B FUS 1-163 QQ4xSS #2

Plasmid

#127195

Purpose

Protein expression for affinity purification

Depositor

Nicolas Fawzi

Article

Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-

Insert

FUS 1-163 QQ4xSS #2 (FUS Human)

Use

Tags

hexahistidine

Expression

Bacterial

Mutation

QQ4xSS #2:Q8S, Q9S, Q60S, Q62S, Q102, Q103, Q139S…

Promoter

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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AT1G35710 X075_pECIA2

Plasmid

#115098

Purpose

Bait vector AT1G35710 X075_pECIA2 should be used with prey vector AT1G35710 X075_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G35710 (AT1G35710 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
AT1G56140 X032_pECIA2

Plasmid

#115062

Purpose

Bait vector AT1G56140 X032_pECIA2 should be used with prey vector AT1G56140 X032_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G56140 (AT1G56140 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
AT1G06840 X062_pECIA2

Plasmid

#115052

Purpose

Bait vector AT1G06840 X062_pECIA2 should be used with prey vector AT1G06840 X062_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G06840 (AT1G06840 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
AT2G19230 M11_pECIA2

Plasmid

#114949

Purpose

Bait vector AT2G19230 M11_pECIA2 should be used with prey vector AT2G19230 M11_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT2G19230 (AT2G19230 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
AT5G48740 O3_pECIA2

Plasmid

#114963

Purpose

Bait vector AT5G48740 O3_pECIA2 should be used with prey vector AT5G48740 O3_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G48740 (AT5G48740 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
AT5G59650 O4_pECIA2

Plasmid

#114964

Purpose

Bait vector AT5G59650 O4_pECIA2 should be used with prey vector AT5G59650 O4_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G59650 (AT5G59650 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
AT1G56120 X070_pECIA2

Plasmid

#115060

Purpose

Bait vector AT1G56120 X070_pECIA2 should be used with prey vector AT1G56120 X070_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G56120 (AT1G56120 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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PXY X058_pECIA2

Plasmid

#115115

Purpose

Bait vector PXY X058_pECIA2 should be used with prey vector PXY X058_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G61480

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
AT2G24130 L4_pECIA2

Plasmid

#115121

Purpose

Bait vector AT2G24130 L4_pECIA2 should be used with prey vector AT2G24130 L4_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT2G24130 (AT2G24130 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge