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pAAV-CAG-EGFP barcode-0-SV40 polyA

Plasmid

#190864

Purpose

For barcoded retrograde labeling

Depositor

Yuejun Chen

Article

Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

Insert

EGFP-barcode0

Use

AAV

Tags

Expression

Mutation

WT

Promoter

Available since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-EGFP barcode-3-SV40 polyA

Plasmid

#190867

Purpose

For barcoded retrograde labeling

Depositor

Yuejun Chen

Article

Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

Insert

EGFP-barcode3

Use

AAV

Tags

Expression

Mutation

WT

Promoter

Available since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-EGFP barcode-4-SV40 polyA

Plasmid

#190868

Purpose

For barcoded retrograde labeling

Depositor

Yuejun Chen

Article

Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

Insert

EGFP-barcode4

Use

AAV

Tags

Expression

Mutation

WT

Promoter

Available since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-EGFP barcode-2-SV40 polyA

Plasmid

#190866

Purpose

For barcoded retrograde labeling

Depositor

Yuejun Chen

Article

Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

Insert

EGFP-barcode2

Use

AAV

Tags

Expression

Mutation

WT

Promoter

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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KL157 - pBlueScript II SK-Grb10_HA-T2A-eGFP-SV40PolyA

Plasmid

#232935

Purpose

Donor plasmid for knock-in of T2A-eGFP-SV40pA at the mouse Grb10 locus. Used with PX459-sgRNA048_Grb10 sgRNA.

Depositor

Danny Reinberg

Article

Lucero et al bioRxiv, March 19, 2025

Insert

T2A-eGFP-SV40pA with Grb10 homology arms

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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single polypeptide FPX biosensor for calcium ion

Plasmid

#60887

Purpose

FPX biosensor

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

single polypeptide FPX biosensor for calcium ion

Use

Tags

HindIII site

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pGP-CAG-iGABASnFR2-WPRE-bGH-polyA

Plasmid

#218868

Purpose

Mammalian expression of improved GABA sensor (positive change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al bioRxiv 2025.03.25.644953

Insert

iGABASnFR2

Use

Tags

Expression

Mammalian

Mutation

S99A F102Y F104Y L178S

Promoter

CAG

Available since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19-TgKI-MCS-eGFP-MCS-polyA

Plasmid

#174024

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Article

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

eGFP

Use

Zebrafish knock-in tagging

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pGP-CAG-iGABASnFR2n-WPRE-bGH-polyA

Plasmid

#218869

Purpose

Mammalian expression of improved GABA sensor (negative change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al bioRxiv 2025.03.25.644953

Insert

iGABASnFR2n

Use

Tags

Expression

Mammalian

Mutation

S99A F104H R168P

Promoter

CAG

Available since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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8xGliBS-IVS2-mCherry-NLS-polyA-Tol2

Plasmid

#84604

Purpose

Tol2-based hedgehog signal reporter plasmid for zebrafish transgenesis

Depositor

James Chen

Article

Mich et al PLoS One. 2014 Jul 28;9(7):e103661. doi:

Insert

mCherry

Use

Zebrafish transgenesis

Tags

Expression

Mutation

Promoter

8xGliBS-delta-crystallin minimal promoter

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-RHO-IntC-dCas9C-VPR-polyA

Plasmid

#166692

Purpose

Express C-terminal part of split dCas9-VPR

Depositor

Stylianos Michalakis

Article

Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

Insert

IntC-dCas9C-VPR

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

short human rhodopsin promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-3xsgRNA_Opn1mw-RHO-Cas9N-IntN-polyA

Plasmid

#165450

Purpose

Express N-terminal part of split dCas9-VPR and 3 sgRNAs targeting murine Opn1mw promoter

Depositor

Elvir Becirovic

Article

Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

Inserts

dCas9N-IntN
3x Opn1mw promoter-targeting sgRNAs

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 and short human rhodopsin promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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PB-3XpolyA-3XPC-miniCMV-mCherry-WPRE-insulator

Plasmid

#168291

Purpose

optimized miniCMV (OminiCMV)

Depositor

Hui Yang

Article

Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

Insert

mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

miniCMV

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19-TgKI-MCS-p2A-tdTomato-polyA

Plasmid

#174027

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Article

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

p2A-tdTomato

Use

Zebrafish knock-in tagging

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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PB-CAG-CasRX-P2A-EGFP-BGH PolyA

Plasmid

#154004

Purpose

Express CasRx

Depositor

Hui Yang

Article

He et al Protein Cell. 2020 Mar 18. pii: 10.1007/

Insert

CasRx

Use

CRISPR

Tags

Expression

Mutation

Promoter

CAG

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19-TgKI-MCS-eGFP_Y40N-MCS-polyA

Plasmid

#174025

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Article

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

eGFP

Use

Zebrafish knock-in tagging

Tags

Expression

Mutation

Y40N

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-EGFP barcode-5-SV40 polyA

Plasmid

#190869

Purpose

For barcoded retrograde labeling

Depositor

Yuejun Chen

Article

Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

Insert

EGFP-barcode5

Use

AAV

Tags

Expression

Mutation

WT

Promoter

Available since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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Polymerase expression - pCMV-Phi29(+exo) (LM2759)

Plasmid

#208964

Purpose

Unfused Phi29 DNA polymerase (+exo), expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

Phi29(+exo)-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19-TgKI-MCS-mScarlet-MCS-polyA

Plasmid

#174023

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Article

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

mScarlet

Use

Zebrafish knock-in tagging

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCIneo-hCHD7-Kozak ATG 3' HA-bGH polyA

Plasmid

#89460

Purpose

constitutive expression of human CHD7 in mammalian cells

Depositor

Peter Scacheri

Insert

CHD7 (CHD7 Human)

Use

Tags

hinge + HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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