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Tol2-LyzC-PBD-GFP-U6ac-ctrl guides

Plasmid

#168252

Purpose

"label active Rac; control for neutrophil-specific knockout"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

PBD GFP

Use

Zebrafish expression

Promoter

LyzC

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-Utrophin-GFP-U6ac-ctrl guides

Plasmid

#168253

Purpose

"label stable F-actin; control for neutrophil-specific knockout"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

Utrophin-GFP

Use

Zebrafish expression

Promoter

LyzC

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDestTol2pA2_ubi:EGFP

Plasmid

#27323

Depositor

Leonard Zon

Article

Mosimann et al Development. 2011 Jan . 138(1):169-77.

Insert

ubiquitin:EGFP reporter

Use

Tol2 transposon

Available Since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only

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pRL056 pDESTattB_exorh:EGFP

Plasmid

#213510

Purpose

attB-containing pDEST plasmid, for use in Multisite Gateway reaction

Depositor

Christian Mosimann

Article

Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

Type

Empty backbone

Use

Zebrafish expression

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCK122 pDESTattB_exorh:mCherry

Plasmid

#213511

Purpose

attB-containing pDEST plasmid, for use in Multisite Gateway reaction

Depositor

Christian Mosimann

Article

Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

Type

Empty backbone

Use

Zebrafish expression

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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p5E-slc1a3b

Plasmid

#170205

Purpose

Gateway 5’ entry vector with the slc1a3b promoter

Depositor

Kelly Monk

Article

Chen et al Nat Neurosci. 2020 Oct;23(10):1297-1306.

Insert

slc1a3b (slc1a3b )

Use

Unspecified; Zebrafish

Promoter

slc1a3b

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR/D_creERt2

Plasmid

#27321

Depositor

Leonard Zon

Article

Mosimann et al Development. 2011 Jan . 138(1):169-77.

Insert

creERt2

Use

Multisite gateway middle entry vector

Available Since

Jan. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pRL092 pattB_cryaa:Venus_MCS

Plasmid

#213513

Purpose

attB-containing vector backbone, for use in restriction enzyme- or PCR-based cloning

Depositor

Christian Mosimann

Article

Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

Type

Empty backbone

Use

Zebrafish expression

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pRL055 pDESTattB_cryaa:Venus

Plasmid

#213509

Purpose

attB-containing pDEST plasmid, for use in Multisite Gateway reaction

Depositor

Christian Mosimann

Article

Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

Type

Empty backbone

Use

Zebrafish expression

Available Since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRL093 pattB_exorh:EGFP_MCS

Plasmid

#213514

Purpose

attB-containing vector backbone, for use in restriction enzyme- or PCR-based cloning

Depositor

Christian Mosimann

Article

Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

Type

Empty backbone

Use

Zebrafish expression

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCK123 pDESTattB_exorh:mCerulean

Plasmid

#213512

Purpose

attB-containing pDEST plasmid, for use in Multisite Gateway reaction

Depositor

Christian Mosimann

Article

Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

Type

Empty backbone

Use

Zebrafish expression

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2TAL3-RR-zrif1_exon8

Plasmid

#194434

Purpose

Right (RR) TALEN for mutating zebrafish rif1 gene

Depositor

Christopher Sansam

Article

Masser et al eLife 2023 (Reviewed Preprint) 12:RP8767

Insert

zrif1_ex8_RIGHT (rif1 Zebrafish)

Use

TALEN

Promoter

CMV IE94

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2TAL3-DD-zrif1_exon8

Plasmid

#194435

Purpose

Left (DD) TALEN for mutating zebrafish rif1 gene

Depositor

Christopher Sansam

Article

Masser et al eLife 2023 (Reviewed Preprint) 12:RP8767

Insert

zrif1_ex8_LEFT (rif1 Zebrafish)

Use

TALEN

Promoter

CMV IE94

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-elavl3_NES-rsCaMPARI-mRuby3

Plasmid

#122129

Purpose

Pan-neuronal expression of rsCaMPARI in zebrafish, nucleus excluded

Depositor

Eric Schreiter

Article

Sha et al Elife. 2020 Sep 15;9. pii: 57249. doi: 1

Insert

rsCaMPARI

Use

Zebrafish expression

Tags

NES-His and mRuby3

Promoter

elavl3

Available Since

Oct. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pTol2-rtn1a-KALTA4

Plasmid

#239989

Purpose

Expresses GAL4 under control of the rtn1a promoter

Depositor

Summer Thyme

Article

Moyer et al bioRxiv [Preprint]. 2025 Mar 5:2025.02.2

Inserts

KALTA4
EGFP

Use

Zebrafish tol2/phic31 compatible

Promoter

myl7 and rtn1a

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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ZE31 Prickle-1-pCS2P+

Plasmid

#17072

Depositor

Randall Moon

Article

Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

Insert

Prickle-1 (prickle1a Zebrafish)

Use

Zebrafish expression

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pKSII/INSeGFP

Plasmid

#53787

Purpose

1 kb zebrafish insulin promoter driving eGFP

Depositor

Larry Moss

Article

diIorio et al Dev Biol. 2002 Apr 1;244(1):75-84.

Insert

INS promoter/eGFP/pA

Use

Zebrafish

Tags

eGFP

Expression

Bacterial

Promoter

zebrafish insulin/ZIP

Available Since

Jan. 7, 2015

Availability

Academic Institutions and Nonprofits only
pISceI Dusp6:D2EGFP

Plasmid

#32667

Depositor

Michael Tsang

Article

Molina et al BMC Dev Biol. 2007 Jun 6;7:62.

Insert

Dusp6 promoter (dusp6 Zebrafish)

Use

Zebrafish transgenesis

Tags

EGFP

Available Since

Nov. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pME-mCD8mCherry

Plasmid

#170206

Purpose

Gateway middle entry vector that encodes mCD8mCherry

Depositor

Kelly Monk

Article

Chen et al Nat Neurosci. 2020 Oct;23(10):1297-1306.

Insert

mCD8

Use

Zebrafish

Tags

mCherry

Promoter

none

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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Zf Slit1a

Plasmid

#61279

Purpose

contains zebrafish Slit1a cDNA

Depositor

Chi-Bin Chien, Lara Hutson

Article

Hutson et al Dev Dyn. 2003 Nov;228(3):358-69.

Insert

Slit1a (slit1a Zebrafish)

Use

In vitro transcription

Available Since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only