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pAc5.1B-EGFP-DmDCP2-V5His6

Plasmid

#22410

Depositor

Elisa Izaurralde

Article

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

decapping protein 2, variant A (DCP2 Fly)

Use

Drosophila expression

Tags

EGFP and V5-His6

Available Since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only

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PGRP-SA

Plasmid

#21055

Depositor

Johann Deisenhofer

Article

Chang et al PLoS Biol. 2004 Sep . 2(9):E277.

Insert

PGRP-SA (PGRP-SA Fly)

Tags

His

Expression

Insect

Available Since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
pAc5.1B-lambdaN-HA-DmEDC3

Plasmid

#21712

Depositor

Elisa Izaurralde

Article

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

Enhancer of decapping 3 (Edc3 Fly)

Use

Drosophila expression

Tags

lambdaN-HA

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmTral

Plasmid

#21721

Depositor

Elisa Izaurralde

Article

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

Trailer hitch (tral Fly)

Use

Drosophila expression

Tags

lambdaN-HA

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pFastBac1 Flag Su(Z)2

Plasmid

#20202

Depositor

Nicole Francis

Article

Lo et al Mol Cell Biol. 2009 Jan . 29(2):515-25.

Insert

Su(Z)2 (Su(z)2 Fly)

Tags

Flag

Expression

Insect

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pBSc polyA + Shal2

Plasmid

#16144

Depositor

Larry Salkoff

Article

Butler et al Science. 1989 Feb 17. 243(4893):943-7.

Insert

Shal (Shal Fly)

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
Stat92e pPAcPL

Plasmid

#8713

Depositor

Jim Darnell

Article

Yan et al Cell 1996 Feb 9;84(3):421-30.

Insert

Stat92e (Stat92E Fly)

Expression

Insect

Available Since

July 20, 2005

Availability

Academic Institutions and Nonprofits only
LifeAct-MTED

Plasmid

#217498

Purpose

Expresses LifeAct-mScarlet-MTED for targeting fluorescent, MT depolymerizing peptide MTED to f-actin

Depositor

Erik Dent

Article

Holland et al Mol Biol Cell. 2024 Jun 1;35(6):mr3. doi

Insert

LifeAct-mScarlet-MTED

Expression

Mammalian

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJFRC-13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest

Plasmid

#217515

Purpose

Encodes a LexAoP controlled and Flp dependent conditional trangene of bicistronic myrGFP and KD Recombinase

Depositor

S. Lawrence Zipursky

Article

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest

Expression

Insect

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pHD-T2A-split-intein-Gal4[21-881]-C-int

Plasmid

#199204

Purpose

To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[21-881]C-int

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-split-intein-Gal4[21-881]-C-int

Expression

Insect

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-DmPABPC1_J

Plasmid

#146647

Purpose

Bacterial Expression of DmPABPC1

Depositor

Elisa Izaurralde

Insert

DmPABPC1 (pAbp Fly)

Expression

Bacterial

Mutation

G26S relative to NM_166265.2/NP_725750.1

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmG3BP_G

Plasmid

#146349

Purpose

Insect Expression of DmG3BP

Depositor

Elisa Izaurralde

Insert

DmG3BP (rin Fly)

Expression

Insect

Mutation

V632G compared to AF231031.1, exact match to NP_5…

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-11-6His-DmBelle_B

Plasmid

#145950

Purpose

Bacterial Expression of DmBelle

Depositor

Elisa Izaurralde

Insert

DmBelle (bel Fly)

Expression

Bacterial

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pACUH-GFP1-10 mitochondrial matrix

Plasmid

#172063

Purpose

UAS vector to express GFP1-10 in the mitochondrial matrix

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

GFP1-10 mitochondrial matrix

Expression

Insect

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pBS-KS-attB1-2-PT-SA-SD-2-mTagBFP2(11x7)

Plasmid

#172066

Purpose

Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 2 coding intron

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

mTagBFP2(11x7)

Expression

Insect

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDmAlfaTub84b

Plasmid

#165781

Purpose

Drosophila Alpha Tubulin promoter

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

AlfaTub84b

Expression

Insect

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pUASTattB-GFP-Msp300KASH

Plasmid

#170806

Purpose

Expresses Msp300 KASH domain fused to GFP under Gal4/UAS control for Drosophila transgenesis

Depositor

Vikki Weake

Article

Jauregui-Lozano et al Genetics. 2021 May 22. pii: 6281219. doi

Insert

GFP-Msp300KASH

Tags

GFP

Expression

Insect

Promoter

5xUAS + hsp70 minimal promoter

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pETM-60-NusA-DmBelle_B

Plasmid

#145963

Purpose

Bacterial Expression of DmBelle

Depositor

Elisa Izaurralde

Insert

DmBelle (bel Fly)

Expression

Bacterial

Mutation

del 155-157, T530A and I685V

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pGC(2.1)-U6.3

Plasmid

#170516

Purpose

PCR template vector for amplifying gRNAcore(2.1)-pU6.3 promoter fragment; used together with pAC-CR7T-gRNA2.1-nlsBFP

Depositor

Chun Han

Article

Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

Insert

gRNAcore(2.1)-pU6.3

Use

Pcr template vector for amplifying grnacore(2.1)-…

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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908H

Plasmid

#160240

Purpose

TetO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attB

Depositor

Omar Akbari

Article

Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

Inserts

Tet Operator (2x)
hsp70 minimal promoter
p-element promoter
Firefly luciferase
Renilla luciferase
Ubiquitin promoter

Tags

T2A GFP

Expression

Insect

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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