3,636 results

1. LN-entry+NLS

   Plasmid

   #128011

   Purpose

   Vector containing the lambdaN-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancer

   Depositor

   Julius Brennecke

   Article

   Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

   Insert

   LN-3xFlag-SV40NLS
   

   Use

   Lamdan entry vector with nls to generate tetherin…

   Tags

   LN-3xFlag-SV40NLS

   Available Since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCFD3-Act5c

   Plasmid

   #130278

   Purpose

   Drosophila expression of sgRNA that targets Actin5c CDS close to stop codon. Positive control sgRNA for CRISPaint knock-ins in cultured cells.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   pCFD3-Act5c (Act5C Fly)
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. OA-1045C

   Plasmid

   #125005

   Purpose

   Expresses gRNAs targeting hid, eve, and wingless

   Depositor

   Omar Akbari

   Article

   Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

   Insert

   eve gRNA (eve Fly)
   

   Expression

   Insect

   Promoter

   U6:3

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWalium-ChtVis-Tomato

   Plasmid

   #67756

   Purpose

   Expresses a fluorescent chitin reporter under Gal4 control in Drosophila.

   Depositor

   Paul Adler

   Article

   Sobala et al Development. 2015 Nov 15;142(22):3974-81

   Insert

   dyl - chitin binding domain - tdTomato - dyl
   

   Tags

   td-tomato

   Expression

   Insect

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAc5.1B-EGFP-DmDCP1

   Plasmid

   #21684

   Depositor

   Elisa Izaurralde

   Article

   Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

   Insert

   Decapping protein 1 (Dcp1 Fly)
   

   Use

   Drosophila expression

   Tags

   EGFP

   Available Since

   Aug. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEM-T Dmel Or43b

   Plasmid

   #59654

   Purpose

   Probe for Drosophila melanogaster Or43b expression

   Depositor

   Richard Axel, Leslie Vosshall

   Article

   Vosshall et al Cell. 1999 Mar 5;96(5):725-36.

   Insert

   Or43b (Or43b Fly)
   

   Expression

   Bacterial

   Available Since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

7. Or1a-Gal4

   Plasmid

   #63019

   Purpose

   To express Gal4 in the pattern of the Drosophila melanogaster odorant receptor Or1a, using the promoter sequences of the gene cloned 5' of Gal4

   Depositor

   Leslie Vosshall

   Article

   Fishilevich et al Curr Biol. 2005 Dec 6;15(23):2086-96.

   Insert

   Drosophila melanogaster Or1a promoter
   

   Tags

   Drosophila ADH (aa's 1-18) fused to Yeast Ga…

   Expression

   Insect

   Available Since

   April 22, 2015

   Availability

   Academic Institutions and Nonprofits only

8. NELFE-mOrange pHis-//

   Plasmid

   #53303

   Purpose

   Expresses His-tagged NELF-E-mOrange fusion protein.

   Depositor

   John Lis

   Article

   Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

   Insert

   NELF-E protein (Nelf-E Fly)
   

   Tags

   His and mOrange

   Expression

   Bacterial

   Available Since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETM-60-NusA-DmBelle_B

   Plasmid

   #145963

   Purpose

   Bacterial Expression of DmBelle

   Depositor

   Elisa Izaurralde

   Insert

   DmBelle (bel Fly)
   

   Expression

   Bacterial

   Mutation

   del 155-157, T530A and I685V

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. 907H1

    Plasmid

    #160234

    Purpose

    FlightN-CymR-VP16-3X-SV40-attB

    Depositor

    Omar Akbari

    Article

    Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

    Inserts

    p-cymene repressor
    Flightin promoter
    

    Tags

    VP16

    Expression

    Insect

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAC-Per

    Plasmid

    #33157

    Depositor

    Michael Rosbash

    Article

    Nawathean et al Mol Cell. 2004 Jan 30;13(2):213-23.

    Insert

    Per (per Fly)
    

    Use

    Retroviral

    Tags

    6xHIS and V5

    Expression

    Insect

    Promoter

    Actin 5C

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pUASTattB_NSnoFbox-vhhGFP4

    Plasmid

    #35578

    Depositor

    Markus Affolter

    Article

    Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

    Insert

    NSnoFbox-vhhGFP4 (slmb Fly)
    

    Expression

    Insect

    Mutation

    F box domain of slmb deleted.

    Available Since

    April 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAC-Cyc

    Plasmid

    #33156

    Depositor

    Michael Rosbash

    Article

    Kadener et al PLoS Biol. 2008 May 20;6(5):e119.

    Insert

    Cyc (cyc Fly)
    

    Use

    Retroviral

    Tags

    6xHIS and V5

    Expression

    Insect

    Promoter

    Actin 5C

    Available Since

    Dec. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

14. K560_AviN_coBirA

    Plasmid

    #129766

    Purpose

    Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated on the head for single-molecule assays

    Depositor

    William Hancock

    Insert

    KHC (Khc Fly)
    

    Tags

    Avi-tag GLNDIFEAQKIEWH and His6

    Expression

    Bacterial

    Mutation

    Truncation at 559

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBSc + MShab

    Plasmid

    #16146

    Depositor

    Larry Salkoff

    Article

    Pak et al J Neurosci. 1991 Mar . 11(3):869-80.

    Insert

    Mus musculus potassium voltage gated channel (Kcnb1 Mouse, Fly)
    

    Use

    Xenopus oocyte expression

    Mutation

    N/A

    Available Since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pBScMXT + dSlo

    Plasmid

    #16173

    Depositor

    Larry Salkoff

    Article

    Wei et al Neuron. 1994 Sep . 13(3):671-81.

    Insert

    Drosophila melanogaster calcium activated potassium channel (slo Fly)
    

    Use

    Xenopus oocyte expression

    Mutation

    N/A

    Available Since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

17. pUAS-kkv-R896K::NG

    Plasmid

    #138957

    Purpose

    Used to generate a transgene that will express an inactive fluorescent Chitin Synthase in Drosophila using Gal4

    Depositor

    Paul Adler

    Article

    Adler et al bioRxiv 2020

    Insert

    kkv-R896K::NG (kkv Fly)
    

    Tags

    Neon Green fused to C terminus of Kkv

    Expression

    Insect

    Mutation

    R896K

    Promoter

    UAS

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-28a-GFP-Patronin CC 868–1457

    Plasmid

    #59053

    Purpose

    for E. coli expression of Patronin CC truncation

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    Patronin (Patronin Fly)
    

    Tags

    His, Strep, TEV, and sfGFP

    Expression

    Bacterial

    Available Since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pCFJ1202

    Plasmid

    #44484

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Insert

    Minimal Mos1, NeoR, peel-1, MCS
    

    Use

    Multiple cloning site vector

    Available Since

    May 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRD119 (PCNA GFP)

    Plasmid

    #14822

    Depositor

    Robert Duronio

    Article

    Thacker et al Curr Biol. 2003 Jan 8. 13(1):53-8.

    Insert

    PCNA-GFP (PCNA Fly)
    

    Tags

    GFP

    Expression

    Bacterial

    Available Since

    July 30, 2007

    Availability

    Academic Institutions and Nonprofits only