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Plasmid#185895PurposeIn vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).DepositorInsertPRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PSk.GAL2>YFAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 25, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUA97
Plasmid#69340PurposeIt encodes a fluorescent protein that can be fused to a gene of interest using SEC CRISPR strategyDepositorInsertceBFP SEC 3xFlag
UseTags3xFlag and ceBFP (mTagBFP)ExpressionWormMutationsqt-1(e1350)PromoterAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLinker-6xHA-HXGPRT-LoxP
Plasmid#86552PurposeThe plasmid is used for endogenous tagging using CRISPR technology in ToxoplasmaDepositorInsertLinker-6xHA-HGPRT-LoxP
UseExpression in toxoplasma gondiiTagsExpressionMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRARB-donor
Plasmid#132517PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertRARB (RARB Human)
UseCRISPRTagsGFPExpressionMutationnonePromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pINER2R
Plasmid#185882PurposeIn vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (11 copy).DepositorInsertPRPL25(Arm 1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm 3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B>RPL25(partial; Arm2)
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pTUB:FLuc
Plasmid#114578PurposeConstitutive expression of firefly luciferase in T. gondii using the alpha tubulin promoter; floxed pyrimethamine selectable marker; UPRT locus flanking sequence to direct integrationDepositorInsertFirefly Luciferase
UseCre/Lox and LuciferaseTagsExpressionMutationPromoteralpha tubulin T. gondiiAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pINER3R
Plasmid#185883PurposeIn vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).DepositorInsertPRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2)
UseTagsExpressionYeastMutationNonePromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIAeBlueHPV16LR
Plasmid#185894PurposeIn vivo gene amplification (HamAmp) of AeBlue + HPV16 L1 expression module at RPL25 locusDepositorInsertPRPL25(Arm1)>Kl.LEU2>TKl.LEU2-TRPL25(Arm3)-ARS305-PALD6>EforRed>TPGK1-PSe.GAL2>HPV16-L1_C-6*H>TRPL41B-PBTS1>RPL25(partial;Arm2)
UseTagsExpressionYeastMutationNonePromoterAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIT7A6
Plasmid#185896PurposeIn vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).DepositorInsertPRPL25(Arm 1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm 3)-ARS305-PSk.GAL2>Y.FAST-EVBR1.2A>TRPL41B-PPDA1>RPL25(partial;Arm2)
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pINER4R
Plasmid#185884PurposeIn vivo gene amplification (HamAmp) of nerolidol synthetic module locus (25 copy).DepositorInsertPRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B-PBTS1>RPL25(part;Arm2)
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTRERF1-donor
Plasmid#176366PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertTRERF1 (TRERF1 Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNR2E3-donor
Plasmid#176369PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertNR2E3 (NR2E3 Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRORB-donor
Plasmid#176354PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertRORB (RORB Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZBED9-donor
Plasmid#176344PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertZBED9 (ZBED9 Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTSC22D3-donor
Plasmid#176355PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertTSC22D3 (TSC22D3 Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZNF22-donor
Plasmid#176349PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertZNF22 (ZNF22 Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pZNF302-donor
Plasmid#132513PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertZNF302 (ZNF302 Human)
UseCRISPRTagsGFPExpressionMutationnonePromoterAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTSHZ1-donor
Plasmid#132479PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertTSHZ1 (TSHZ1 Human)
UseCRISPRTagsGFPExpressionMutationnonePromoterAvailable sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB-Bleo Gal4 tBFP
Plasmid#139190PurposeGal4-inducible tag blue fluorescent protein (tBFP) in pSB-Bleo Gal4 Sleeping Beauty backbone.DepositorInserttBFP
UseTransposonTagsExpressionMammalianMutationPromoterGal4 CMV and STAT3Available sinceAvailabilityAcademic Institutions and Nonprofits only -
hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(1-100)Cy-
Plasmid#191390PurposeDonor for targeted integration of hCdt1(1-100)Cy- probe to the hROSA26 safe harbor locusDepositorInserthCdt1(1-100)Cy-
UseTagsmScarlet-IExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
AbVec2.0-mIghg2c
Plasmid#127159PurposeExpression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG2c isotypeDepositorInsertIghg2c (Ighg2c Mouse)
UseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only