We narrowed to 4,504 results for: pcr

1. pCRISPR/eCas9-GPIDAF

   Plasmid

   #213710

   Purpose

   To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIDAF under separate promoters.

   Depositor

   Qilai Huang

   Article

   Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   3XFLAG and Twin-strep-tag

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCRISPR/eCas9-GPIBY55

   Plasmid

   #213709

   Purpose

   To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIBY55 under separate promoters.

   Depositor

   Qilai Huang

   Article

   Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   3XFLAG and Twin-strep-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PCRbluntII-pHES7-STOPgRNA

   Plasmid

   #204349

   Purpose

   gRNA to target pig HES7 stop codon

   Depositor

   Li-Fang Chu

   Article

   Conrad et al Stem Cell Reports. 2023 Dec 12;18(12):23

   Insert

   Pig HES7-STOP-gRNA (HES7 Sus domesticus (pig))
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCRISPRa_all-in-one_Bip gRNA1

   Plasmid

   #183696

   Purpose

   Expresses gRNA targeting chinise hamster Bip (Hspa5) with CRISPRa components and mCherry, blasticidin selective

   Depositor

   Edward Avezov

   Article

   Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

   Insert

   gRNA targeting chinese hamster Bip (Hspa5)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCRII-TOPO-DrSmg1_F

   Plasmid

   #146277

   Purpose

   Bacterial Expression of DrSmg1

   Depositor

   Elisa Izaurralde

   Article

   Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

   Insert

   DrSmg1 (smg1 Zebrafish)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCRII-TOPO-DrSmg5_F

   Plasmid

   #146278

   Purpose

   Bacterial Expression of DrSmg5

   Depositor

   Elisa Izaurralde

   Article

   Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

   Insert

   DrSmg5 (smg5 Zebrafish)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCRII-TOPO-DrSmg6_F

   Plasmid

   #146279

   Purpose

   Bacterial Expression of DrSmg6

   Depositor

   Elisa Izaurralde

   Article

   Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

   Insert

   DrSmg6 (LOC100334020 Zebrafish)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCRII-TOPO-DrSmg7_F

   Plasmid

   #146280

   Purpose

   Bacterial Expression of DrSmg7

   Depositor

   Elisa Izaurralde

   Article

   Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

   Insert

   DrSmg7 (smg7 Zebrafish)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCRII-TOPO-DrUPF1_F

   Plasmid

   #146281

   Purpose

   Bacterial Expression of DrUPF1

   Depositor

   Elisa Izaurralde

   Article

   Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

   Insert

   DrUPF1 (upf1 Zebrafish)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCRII-TOPO-DrUPF2_F

    Plasmid

    #146282

    Purpose

    Bacterial Expression of DrUPF2

    Depositor

    Elisa Izaurralde

    Article

    Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

    Insert

    DrUPF2 (upf2 Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCRII-TOPO-DrUPF3a_F

    Plasmid

    #146283

    Purpose

    Bacterial Expression of DrUPF3a

    Depositor

    Elisa Izaurralde

    Article

    Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

    Insert

    DrUPF3a (upf3a Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCRII-TOPO-DrUPF3b_F

    Plasmid

    #146284

    Purpose

    Bacterial Expression of DrUPF3b

    Depositor

    Elisa Izaurralde

    Article

    Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

    Insert

    DrUPF3b (upf3b Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCRISPR2_+10D+10U

    Plasmid

    #149394

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 10bp addition upstream of IHFprox site

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    10bp inserted upstream and downstream of the IHFp…

    Promoter

    pBAD

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcryGFP-acta1-dmdGFP

    Plasmid

    #172530

    Purpose

    alphaA-crystallin promoter (cry) drives GFP expression in the lens. The acta1 muscle-specific promoter drives expression of GFP-tagged zebrafish dmd.

    Depositor

    Joachim Berger

    Article

    Berger et al J Cell Mol Med. 2020 Jun;24(12):6680-668

    Insert

    dmd (dmd Zebrafish)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCR3.1-CREM2 luc

    Plasmid

    #106460

    Purpose

    luciferase reporter, CMV promoter (CRE site adjacent to NFkB site mutated)

    Depositor

    Georg Weber

    Article

    He et al Biochem Biophys Res Commun. 2004 Aug 13;

    Insert

    luciferase reporter, CMV promoter (CRE site adjacent to NFkB site mutated)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    CRE site adjacent to NFkB site mutated

    Promoter

    Available since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCri-H6-TEV-GRAB

    Plasmid

    #128498

    Purpose

    Expresses GRAB (from V34 to N181) (Streptococcus pyogenes serotype M1) in bacterial cells. With N-t H6x tag + TEV.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    GRAB (Streptococcus pyogenes serotype M1) (grab Streptococcus pyogenes serotype M1)
    

    Use

    Tags

    His tag (6x)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PCRII apoA-IVb.1

    Plasmid

    #121903

    Purpose

    Plasmid for making zebrafish apoA-IVb.1 in situ probe

    Depositor

    Steven Farber

    Article

    Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

    Insert

    Apoa4b.1 (apoa4b.1 Zebrafish)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PCRII apoA-IVb.3

    Plasmid

    #121905

    Purpose

    Plasmid for making zebrafish apoA-IVb.3 in situ probe

    Depositor

    Steven Farber

    Article

    Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

    Insert

    apoa4b.3 (apoa4b.3 Zebrafish)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PCRII apoA-IVa

    Plasmid

    #121902

    Purpose

    Plasmid for making zebrafish apoA-IVa in situ probe

    Depositor

    Steven Farber

    Article

    Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

    Insert

    ApoA4a (apoa4a Zebrafish)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PCRII apoA-IVb.2

    Plasmid

    #121904

    Purpose

    Plasmid for making zebrafish apoA-IVb.2 in situ probe

    Depositor

    Steven Farber

    Article

    Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

    Insert

    Apoa4b.2 (apoa4b.2 Zebrafish)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCR3.1-OPNc PSM1

    Plasmid

    #106328

    Purpose

    Mammalian expression of mutant osteopontin

    Depositor

    Georg Weber

    Article

    Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

    Insert

    OPNc (SPP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    OPNc phosphorylation site mutant SGSSEEKQNAVSSEET…

    Promoter

    Available since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCR3.1-OPNc PSM3

    Plasmid

    #106327

    Purpose

    Mammalian expression of mutant osteopontin

    Depositor

    Georg Weber

    Article

    Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

    Insert

    OPNc (SPP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    OPNc phosphorylation site mutant SGSSEEKQNAVSSEET…

    Promoter

    Available since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCR3.1-OPNc PSM2

    Plasmid

    #106326

    Purpose

    Mammalian expression of mutant osteopontin

    Depositor

    Georg Weber

    Article

    Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

    Insert

    OPNc (SPP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    OPNc phosphorylation site mutant SGSSEEKQNAVSSEET…

    Promoter

    Available since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCR3.1-OPNc PSM4

    Plasmid

    #106329

    Purpose

    Mammalian expression of mutant osteopontin

    Depositor

    Georg Weber

    Article

    Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

    Insert

    OPNc (SPP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    OPNc phosphorylation site mutant SGSSEEKQNAVSSEET…

    Promoter

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCRRNA-G10-R20

    Plasmid

    #101819

    Purpose

    Repress mCherry and GFP independently, with different strengths

    Depositor

    Sven van Teeffelen, David Bikard

    Article

    Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

    Insert

    crRNAs targeting GFP and mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Spy 1049

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCRRNA-G14-R20

    Plasmid

    #101821

    Purpose

    Repress mCherry and GFP independently, with different strengths

    Depositor

    Sven van Teeffelen, David Bikard

    Article

    Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

    Insert

    crRNAs targeting GFP and mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Spy 1049

    Available since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCRRNA-G10-R11

    Plasmid

    #101809

    Purpose

    Repress mCherry and GFP independently, with different strengths

    Depositor

    Sven van Teeffelen, David Bikard

    Article

    Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

    Insert

    crRNAs targeting GFP and mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Spy 1049

    Available since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCRRNA-G11-R20

    Plasmid

    #101820

    Purpose

    Repress mCherry and GFP independently, with different strengths

    Depositor

    Sven van Teeffelen, David Bikard

    Article

    Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

    Insert

    crRNAs targeting GFP and mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Spy 1049

    Available since

    Oct. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCRRNA-G20-R18

    Plasmid

    #101817

    Purpose

    Repress mCherry and GFP independently, with different strengths

    Depositor

    Sven van Teeffelen, David Bikard

    Article

    Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

    Insert

    crRNAs targeting GFP and mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Spy 1049

    Available since

    Oct. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCRRNA-G14-R18

    Plasmid

    #101816

    Purpose

    Repress mCherry and GFP independently, with different strengths

    Depositor

    Sven van Teeffelen, David Bikard

    Article

    Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

    Insert

    crRNAs targeting GFP and mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Spy 1049

    Available since

    Oct. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge