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Addgene

We narrowed to 772 results for: tev cleavage

Showing: 601 - 640 of 772 results
  1. DMTF1

    Plasmid
    #36895
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    DMTF1 (DMTF1 Human)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    contains Myb-like 1 domain, residues 220-274
    Promoter
    Available since
    June 19, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
  2. L284F Cus2p

    Plasmid
    #73059
    Purpose
    S. cerevisiae Cus2p gene with L284F Mutation
    Depositor
    Insert
    CUS2p (CUS2 Budding Yeast)
    Use
    Tags
    6x His tag fused to TEV cleavage site
    Expression
    Bacterial and Yeast
    Mutation
    Leucine 284 to Phenylalanine
    Promoter
    T7
    Available since
    Availability
    Academic Institutions and Nonprofits only
  3. D282N Cus2p

    Plasmid
    #73060
    Purpose
    S. cerevisiae Cus2p gene with D282N Mutation
    Depositor
    Insert
    CUS2p (CUS2 Budding Yeast)
    Use
    Tags
    6x His tag fused to TEV cleavage site
    Expression
    Bacterial and Yeast
    Mutation
    Asp 282 to Asn
    Promoter
    T7
    Available since
    Availability
    Academic Institutions and Nonprofits only
  4. FnR9-10

    Plasmid
    #182567
    Purpose
    Human fibronectin fragment, type-III repeats 9-10, R1379A mutant
    Depositor
    Insert
    Human fibronectin fragment, type-III repeats 9-10
    Use
    Tags
    His and TEV cleavage site after His tag
    Expression
    Bacterial
    Mutation
    R1379A
    Promoter
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  5. pTet-On-TAP

    Plasmid
    #59017
    Purpose
    Construct allows tetracycline inducible expression of a protein of interest fused to a tandem affinity purification tag (TAP) in toxoplasma gondii.
    Depositor
    Type
    Empty backbone
    Use
    Toxoplasma expression
    Tags
    Calmodulin Binding Peptide fused with a TEV Cleav…
    Expression
    Mutation
    Promoter
    RPS13 with Tet Operators Inserted
    Available since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
  6. dFP-mini

    Plasmid
    #120543
    Purpose
    Expresses dFP biliverdin-binding fluorescence-activating mini 10 KDa protein in bacteria.
    Depositor
    Insert
    de novo fluorescence-activating protein mini (dFP-mini)
    Use
    Tags
    6xHis - TEV cleavage
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
  7. pEG584

    Plasmid
    #40191
    Depositor
    Insert
    Mex-5 (RNAi resistant) (mex-5 Nematode)
    Use
    Tags
    Dendra2 PAFP (sequence optimized for worm express…
    Expression
    Worm
    Mutation
    Exon 2 recoded to be RNAi-resistant
    Promoter
    4.4 kb mex-5 promoter fragment
    Available since
    Sept. 5, 2012
    Availability
    Academic Institutions and Nonprofits only
  8. pEG587

    Plasmid
    #40196
    Depositor
    Insert
    Mex-5 (S458A) (RNAi resistant) (mex-5 Nematode)
    Use
    Tags
    Dendra2 PAFP (sequence optimized for worm express…
    Expression
    Worm
    Mutation
    S458A; exon 2 recoded to be RNAi-resistant
    Promoter
    4.4 kb mex-5 promoter fragment
    Available since
    Sept. 19, 2012
    Availability
    Academic Institutions and Nonprofits only
  9. pEG610

    Plasmid
    #40195
    Depositor
    Insert
    Mex-5 (aa1-355) (RNAi resistant) (mex-5 Nematode)
    Use
    Tags
    Dendra2 PAFP (sequence optimized for worm express…
    Expression
    Worm
    Mutation
    contains aa 1-355; exon 2 recoded to be RNAi-resi…
    Promoter
    4.4 kb mex-5 promoter fragment
    Available since
    Sept. 20, 2012
    Availability
    Academic Institutions and Nonprofits only
  10. pEG585

    Plasmid
    #40192
    Depositor
    Insert
    Mex-5 (S404A) (RNAi resistant) (mex-5 Nematode)
    Use
    Tags
    Dendra2 PAFP (sequence optimized for worm express…
    Expression
    Worm
    Mutation
    S404A; exon 2 recoded to be RNAi-resistant
    Promoter
    4.4 kb mex-5 promoter fragment
    Available since
    Sept. 13, 2012
    Availability
    Academic Institutions and Nonprofits only
  11. FnR8-10

    Plasmid
    #182569
    Purpose
    Human fibronectin fragment, type-III repeats 8-10, R1379A mutant
    Depositor
    Insert
    Human fibronectin fragment, type-III repeats 8-10
    Use
    Tags
    His and TEV cleavage site after His tag
    Expression
    Bacterial
    Mutation
    R1379A
    Promoter
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  12. pBEL2265

    Plasmid
    #175962
    Purpose
    For expresson of LetA-[LetB PLL3->6] operon from E. coli. N-terminal his tag on LetA, araBAD promoter
    Inserts
    LetA
    LetB
    Use
    Tags
    8xHis 2xQH and TEV cleavage site on LetA
    Expression
    Bacterial
    Mutation
    Δ347-361, replace with 702-716
    Promoter
    araBAD
    Available since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 601 - 640 of 772 results