1. p426 103Q GPD

   Plasmid

   #1184

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

These results may also be relevant.

1. pcDNA3 HA Arf6 ActQ67L

   Plasmid

   #10835

   Depositor

   Thomas Roberts

   Article

   Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

   Insert

   Arf6 Q67L (ARF6 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Q67L. Defective in GTP hydrolysis. Constitutive…

   Promoter

   Available since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CyPET-Arf6

   Plasmid

   #18840

   Depositor

   Martin Schwartz

   Article

   Hall et al Anal Biochem. 2008 Mar 15. 374(2):243-9.

   Insert

   CyPET-Arf6 (ARF6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   The sequence for the fluorescent protein CyPET wa…

   Promoter

   Available since

   Aug. 12, 2008

   Availability

   Academic Institutions and Nonprofits only

3. pTriEx-RhoA FLARE.sc Biosensor Q63L

   Plasmid

   #12151

   Depositor

   Klaus Hahn

   Article

   Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

   Insert

   RhoA Biosensor Q63L (RHOA Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   RhoA Q63L (dominant-positive mutation)

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTriEx-RhoA FLARE.sc Biosensor T19N

   Plasmid

   #12152

   Depositor

   Klaus Hahn

   Article

   Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

   Insert

   RhoA Biosensor T19N (RHOA Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   RhoA T19N (dominant-negative mutation)*

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTriEX-Antenna-GDI RhoA

   Plasmid

   #189669

   Purpose

   Expression of Antenna-RhoA

   Depositor

   Klaus Hahn

   Article

   Hodgson et al Nat Chem Biol. 2016 Aug 8. doi: 10.1038/

   Insert

   RhoA (RHOA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDNAIIIB rhoA QL

   Plasmid

   #61632

   Purpose

   mammalian expression of rhoA constitutively active mutant

   Depositor

   JS Gutkind

   Article

   Coso et al Cell. 1995 Jun 30;81(7):1137-46.

   Insert

   rhoA (RHOA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q63L, constitutively active

   Promoter

   CMV

   Available since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pCAG/hArf4(WT)-mCherry

   Plasmid

   #79406

   Purpose

   Expresses C-terminally mCherry-tagged Arf4(WT) in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Nakai et al Mol Biol Cell. 2013 Aug;24(16):2570-81.

   Insert

   Arf4 (ARF4 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

8. pCAG/hArf4(T31N)-HA

   Plasmid

   #79405

   Purpose

   Expresses C-terminally HA-tagged Arf4(T31N) in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Nakai et al Mol Biol Cell. 2013 Aug;24(16):2570-81.

   Insert

   Arf4 (ARF4 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   T31N

   Promoter

   Available since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

9. pCAG/hArf4(Q71L)-HA

   Plasmid

   #79404

   Purpose

   Expresses C-terminally HA-tagged Arf4(Q71L) in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Nakai et al Mol Biol Cell. 2013 Aug;24(16):2570-81.

   Insert

   Arf4 (ARF4 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Gln 71 Leu

   Promoter

   Available since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

10. pDONR221-ARF6

    Plasmid

    #67314

    Purpose

    hArf6 with stop codon in Gateway Entry vector

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARF6 (ARF6 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    None

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pCAG/hArf4(WT)-HA

    Plasmid

    #79403

    Purpose

    Expresses C-terminally HA-tagged Arf4(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Nakai et al Mol Biol Cell. 2013 Aug;24(16):2570-81.

    Insert

    Arf4 (ARF4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

12. pDSHA-ARF5-Myc

    Plasmid

    #67351

    Purpose

    Mammalian expression of hArf5 with C-term Myc tag

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARF5 (ARF5 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pIS1-wt RhoA 3'UTR

    Plasmid

    #26089

    Depositor

    Bob Weinberg

    Article

    Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

    Insert

    wt RhoA 3'UTR (RHOA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pDEST47-ARF4

    Plasmid

    #67455

    Purpose

    Mammalian expression of hArf4 with stop codon (native expression)

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARF4 (ARF4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pDONR221-ARF3

    Plasmid

    #67308

    Purpose

    hArf3 with stop codon in Gateway Entry vector

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARF3 (ARF3 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    None

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pCAG/hArf4(WT)-EGFP

    Plasmid

    #79407

    Purpose

    Expresses C-terminally EGFP-tagged Arf4(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Nakai et al Mol Biol Cell. 2013 Aug;24(16):2570-81.

    Insert

    Arf4 (ARF4 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

17. pDONR221-ARF4

    Plasmid

    #67310

    Purpose

    hArf4 with stop codon in Gateway Entry vector

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARF4 (ARF4 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    None

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pDONR221-ARF5

    Plasmid

    #67312

    Purpose

    hArf5 with stop codon in Gateway Entry vector

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARF5 (ARF5 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Bacterial

    Mutation

    bp 477 T to C; silent mutation

    Promoter

    None

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

19. MZB224

    Plasmid

    #226998

    Purpose

    Bacterial expression of 10xHis-SUMO (Smt3) fusion to HRV-3C cleavable Halotagged Arf (T31N) for pulldown assays

    Depositor

    Richard Baker

    Article

    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Insert

    Arf1 (ARF1 Human)
    

    Use

    Tags

    10x His, HaloTag, and SUMO (SMT3)

    Expression

    Bacterial

    Mutation

    T31N

    Promoter

    T7

    Available since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. RhoA gRNA #1

    Plasmid

    #198718

    Purpose

    Cas9-mediated knockout of RhoA in mammalian cells #1

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    RhoA (Rhoa Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge