We narrowed to 17,072 results for: EMB

1. TRAPa(W255A,N262E,K266E)-3xFLAG-pCDNA

   Plasmid

   #201968

   Purpose

   Mammalian expression of mutated Translocon-associated protein subunit alpha

   Depositor

   Ville Paavilainen

   Article

   Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

   Insert

   SSR1 (SSR1 Human)
   

   Tags

   3xFLAG-tag

   Expression

   Mammalian

   Mutation

   W255A,N262E,K266E

   Available Since

   Jan. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFR093

   Plasmid

   #134149

   Purpose

   Expression of CENP-T

   Depositor

   Iain Cheeseman

   Article

   Nishino et al EMBO J. 2013 Feb 6;32(3):424-36. doi: 10

   Insert

   CENP-T (CENPT Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   aa 1-375 with T85D mutation

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. EGFP-hPMCA2w/b

   Plasmid

   #47586

   Depositor

   Emanuel Strehler

   Article

   Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

   Insert

   PMCA2w/b (ATP2B2 Human)
   

   Tags

   EGFP-HA-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-Hyg-Dest-mCherry-PAR-6B

   Plasmid

   #194908

   Purpose

   To generate mCherry-PAR-6B (mouse) expressing stable cell lines with piggybac transposase co-expression and hygromycin selections.

   Depositor

   Clare Buckley

   Article

   Liang et al EMBO J. 2022 Aug 22:e111021. doi: 10.152

   Insert

   PAR-6B (Pard6b Mouse)
   

   Use

   Mouse Targeting

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   39 C to G, silent mutation

   Available Since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. N-BLa 1.2

   Plasmid

   #88999

   Purpose

   BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the N-terminal fragment of the split β-lactamase.

   Depositor

   Dieter Langosch

   Article

   Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

   Insert

   N-BLa 1.2 fusion protein
   

   Tags

   Flag

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. C-BLa 1.2

   Plasmid

   #89000

   Purpose

   BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the C-terminal fragment of the split β-lactamase.

   Depositor

   Dieter Langosch

   Article

   Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

   Insert

   C-BLa 1.2 fusion protein
   

   Tags

   Flag

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQlinkH-PTPIP51(36-235)

   Plasmid

   #170533

   Purpose

   Expresses Cleavable His-tagged PTPIP51_ΔTPR domain (36-235) in E. Coli

   Depositor

   Byung Il Lee

   Article

   Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

   Insert

   Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   T5 promoter

   Available Since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. mEos3.2GPI

   Plasmid

   #203759

   Purpose

   Encodes the fluorescent protein mEos3.2 followed by the C-terminal sequence derived from CD58 encoding a GPI-attachment signal. Used as a fluorescent plasma membrane probe.

   Depositor

   Sarah Veatch

   Article

   Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

   Insert

   CD58 c-term (CD58 Human)
   

   Tags

   mEos3.2

   Expression

   Mammalian

   Available Since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. EGFP-hPMCA2x/b

   Plasmid

   #47585

   Depositor

   Emanuel Strehler

   Article

   Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

   Insert

   pMCA2x/b (ATP2B2 Human)
   

   Tags

   EGFP-HA-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

10. p-mCherry2-sgMUC4

    Plasmid

    #198399

    Purpose

    Expresses a guide RNA against a repetitive locus found in the MUC4 gene of human chromosome 3, with mCherry2 reporter

    Depositor

    Sarah McClelland

    Article

    Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

    Insert

    MUC4 sgRNA (MUC4 Human)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. EGFP-hPMCA2z/b

    Plasmid

    #47584

    Depositor

    Emanuel Strehler

    Article

    Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

    Insert

    pMCA2z/b (ATP2B2 Human)
    

    Tags

    EGFP-HA-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

12. N-BLa 1.1

    Plasmid

    #88994

    Purpose

    BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.1 fusionprotein containing the N-terminal fragment of the split β-lactamase.

    Depositor

    Dieter Langosch

    Article

    Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

    Insert

    N-BLa 1.1 fusion protein
    

    Tags

    Flag

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG eGFP-Oct4

    Plasmid

    #203789

    Purpose

    Competitive assay of Oct4 and Oct6 binding to DNA motifs.

    Depositor

    Thorsten Wohland

    Article

    Mistri et al EMBO Rep. 2015 Sep;16(9):1177-91. doi: 1

    Insert

    Pou5f1 (Pou5f1 Mouse)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    July 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. C-BLa 1.1

    Plasmid

    #88997

    Purpose

    BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.1 fusionprotein containing the C-terminal fragment of the split β-lactamase.

    Depositor

    Dieter Langosch

    Article

    Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

    Insert

    C-BLa 1.1 fusion protein
    

    Tags

    Flag

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-Dest47- WTN23C11orf83-GFP

    Plasmid

    #65845

    Purpose

    Mammalian expression of the wild type N terminal part (N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)

    Depositor

    Amos Bairoch

    Article

    Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

    Insert

    Transmembrane (23 AA) of UQCC3 (UQCC3 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    WT Transmembrane

    Available Since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEF-DEST51 GFP-PNRC1

    Plasmid

    #123295

    Purpose

    Mammalian expression of PNRC1 N-GFP

    Depositor

    Giovanni Tonon

    Article

    Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

    Insert

    PNRC1 (PNRC1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG eGFP-Oct6

    Plasmid

    #203790

    Purpose

    Competitive assay of Oct4 and Oct6 binding to DNA motifs.

    Depositor

    Thorsten Wohland

    Article

    Mistri et al EMBO Rep. 2015 Sep;16(9):1177-91. doi: 1

    Insert

    Pou3f1 (Pou3f1 Mouse)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. myc-Miro1 (lacking TM)

    Plasmid

    #127613

    Purpose

    Human Miro1 with the transmembrane deleted

    Depositor

    Josef Kittler

    Article

    Lopez-Domenech et al EMBO J. 2018 Feb 1;37(3):321-336. doi: 1

    Insert

    Miro1 (RHOT1 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Deletion of transmembrane domain

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. PCUP1-GFP-Pho8-406

    Plasmid

    #207006

    Purpose

    Integration of GFP-Pho8 as an additional copy in genome, expressed under CUP1 promoter. Uses auxotrophic marker URA3 (Saccharomyces cerevisiae) for selection. Marker for yeast vacuolar membrane.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    PHO8
    

    Tags

    GFP

    Expression

    Yeast

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. ptAD-seq-ubi63E-Gal4-DBD

    Plasmid

    #111930

    Purpose

    Vector to express tAD candidates from a library cloned from fragmented transcription factor coding sequences fused to the Gal4 DNA binding domain.

    Depositor

    Alexander Stark

    Article

    Arnold et al EMBO J. 2018 Aug 15;37(16). pii: embj.20

    Insert

    tAD-seq Gal4-DBD
    

    Expression

    Insect

    Available Since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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