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Plasmid#123194Purposebinary plant vector for transient dual luciferase (rLUC & fLUC with introns) expressionDepositorInsertpNOS::rLUC-I::tNOS tOCS::fLUC-I::pSTR1
UseLuciferaseTagsExpressionPlantMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJEP305-pAAV-EFS-dSaCas9-KRAB-pA
Plasmid#113681PurposeA EFS driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.DepositorInsertde-catalyzed SaCas9
UseAAV and CRISPRTagsKRAB and NLSExpressionMutationPromoterAvailable sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJEP303-pAAV-CMV-dSaCas9-VP64-pA
Plasmid#113678PurposeA CMV driven de-catalyzed SaCas9 fused to VP64 domain for increased transcription in targeted regionDepositorInsertde-catalyzed SaCas9
UseAAV, CRISPR, and Synthetic BiologyTagsNLS and VP64ExpressionMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/FLAG-Ino80 ΔN (267-1556)
Plasmid#29440DepositorInsertINO80 (INO80 Human)
UseTagsFlagExpressionMammalianMutationINO80 267-1556 (a.a.)PromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/FLAG-Ino80 ΔN-EQ (267-1556)
Plasmid#29438DepositorInsertINO80 (INO80 Human)
UseTagsFlagExpressionMammalianMutationINO80 267-1556 (a.a.) E653QPromoterAvailable sinceJuly 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/Flag-hINO80 ΔN ΔHSA (520-1556)
Plasmid#29446DepositorInsertINO80 (INO80 Human)
UseTagsFlagExpressionMammalianMutationINO80 520-1556 (a.a.)PromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/FLAG-Ino80 ΔNC (267-1261)
Plasmid#29439DepositorInsertINO80 (INO80 Human)
UseTagsFlagExpressionMammalianMutationINO80 267-1261 (a.a.) with Q1116K mutationPromoterAvailable sinceAug. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/FLAG-Ino80 ΔNC-EQ (267-1261)
Plasmid#29437DepositorInsertINO80 (INO80 Human)
UseTagsFlagExpressionMammalianMutationINO80 267-1261 (a.a.) E653QPromoterAvailable sinceJuly 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
Danio alpha Bb-crystallin 1kb promoter/AcGFP
Plasmid#98098PurposeaBb-crystallin promoter fragment in GFP plasmid to assess activity in larval embryosDepositorInsertAlpha Bb-crystallin promoter 1 kb fragment, Danio rerio (cryabb Zebrafish)
UseGfp expressingTagsGFPExpressionMutationPromoterDanio aBb-crystallin 1 kb fragment (-1074/-1)Available sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/Flag-hINO80 HSA (212-526)
Plasmid#29443DepositorInsertINO80 (INO80 Human)
UseTagsFlagExpressionMammalianMutationINO80 212-526 (a.a.)PromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/Flag-hINO80C (1224-1556)
Plasmid#29445DepositorInsertINO80 (INO80 Human)
UseTagsFlagExpressionMammalianMutationINO80 1224-1556 (a.a.)PromoterAvailable sinceAug. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-drN-hsAgo2
Plasmid#221724PurposeExpression of human Ago2 with N-terminal 3xFLAG tag in zebrafish embryos. Swapped N-domain for zebrafish Ago2 N-domain.DepositorInsertdrN-hsAgo2 (AGO2 Human)
UseFish expressionTags3xFLAGExpressionMutationSwap of N-domain for zebrafish Ago2 N-domainPromotersimian CMV IE94Available sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-hsN-drAgo2
Plasmid#221717PurposeExpression of zebrafish Ago2 with N-terminal 3xFLAG tag in zebrafish embryos. Swapped N-domain for human Ago2 N-domain.DepositorInserthsN-drAgo2 (ago2 Zebrafish)
UseFish expressionTags3xFLAGExpressionMutationSwap of N-domain for human Ago2 N-domainPromotersimian CMV IE94Available sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-hsAgo2EF-DY
Plasmid#221723PurposeExpression of human Ago2 with E637D and F666Y mutations and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInserthsAgo2EF-DY (AGO2 Human)
UseFish expressionTags3xFLAGExpressionMutationE637D and F666Y in active sitePromotersimian CMV IE94Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-SHMY-A1Pi
Plasmid#182648PurposeMammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), one tyrosine sulfation (TS) motif (Y), myc (M), His6 (H) and A1Pi sequenceDepositorInsertA1Pi (SERPINA1 Human)
UseRetroviralTagsHis6-tag, N-terminal ER targeting sequence of hem…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSB139 - pL0_ZCT1 (CDS1)
Plasmid#123184PurposeGolden Gate (MoClo; CDS1) compatible ZCT1 gene from Catharanthus roseus; a repressor of the STR1 promoter from C. roseusDepositorInsertZCT1 from Catharanthus roseus
UsePart for plant expressionTagsExpressionPlantMutationNo BpiI and BsaI sitesPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB142 - pL0_ORCA3 (CDS1)
Plasmid#123185PurposeGolden Gate (MoClo; CDS1) compatible ORCA3 gene from Catharanthus roseus; an activator of the STR1 promoter from C. roseusDepositorInsertORCA3 from Catharanthus roseus
UsePart for plant expressionTagsExpressionPlantMutationNo BpiI and BsaI sitesPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-1xTS
Plasmid#109418PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to one tyrosine sulfation (TS) motif. VHH-1xTS also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, T7, and Tyrosine sulfation (TS) se…ExpressionBacterialMutationPromoterT7Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
Zebrafish aBb crystallin 5kb promoter/AcGFP
Plasmid#98101PurposeZebrafish aBb crystallin 5kb promoter segment driving GFP expressionDepositorInsertAlpha Bb-crystallin promoter 5 kb fragment, Danio rerio (cryabb Zebrafish)
UseGfp expressingTagsGFPExpressionMutationPromoterDanio alpha Bb-crystallin 5 kb promoter fragment …Available sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
Zebrafish aBb crystallin 4kb promoter/AcGFP
Plasmid#98100PurposeZebrafish aBb crystallin 4kb promoter segment driving GFP expressionDepositorInsertAlpha Bb-crystallin promoter 4 kb fragment, Danio rerio (cryabb Zebrafish)
UseGfp expressingTagsGFPExpressionMutationPromoterDanio alpha Bb-crystallin 4 kb promoter fragment …Available sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only