Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 634 results for: MT3;

Clear keywords
Showing: 301 - 320 of 634 results

  4. (SUMO)5-(SIM)10

    Plasmid
    #126923
    Purpose
    Bacterial expression plasmid for multivalent scaffold of (SUMO)5-(SIM)10 biomolecular condensates to recruit SUMO-containing client proteins.
    Depositor
    Michael Rosen
    Article
    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1
    Insert
    5 repeats of human SUMO3 and 10 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (SUMO3 Human)
    Expression
    Bacterial
    Mutation
    internal BamHI site between (SUMO)5 and (SIM)10
    Promoter
    tac
    Available Since
    Sept. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    126923-plasmid-map-sequence-id-255302

    Enlarge

  11. RFP-(SUMO)6-(SIM)10

    Plasmid
    #122027
    Purpose
    Mammalian expression plasmid for multivalent scaffold of (SUMO)6-(SIM)10 biomolecular condensates to recruit SUMO-containing client proteins.
    Depositor
    Michael Rosen
    Article
    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1
    Insert
    6 repeats of human SUMO3 and 10 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (PIAS2, SUMO3 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    122027-plasmid-map-sequence-id-434238

    Enlarge

  12. RFP-(SUMO)10-(SIM)6

    Plasmid
    #122026
    Purpose
    Mammalian expression plasmid for multivalent scaffold of (SUMO)10-(SIM)6 biomolecular condensates to recruit SIM-containing client proteins.
    Depositor
    Michael Rosen
    Article
    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1
    Insert
    10 copies of human SUMO3 and 6 repeats of human SUMO interactive motif (SIM) from PIASx separated by (GGS)4 linker (SUMO3, PIAS2 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    122026-plasmid-map-sequence-id-255492

    Enlarge

  15. TRIPZ-bioGEF-SUMO1nc-PURO

    Plasmid
    #208042
    Purpose
    Enables inducible expression of low-affinity Avitag-SUMO1nc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin
    Depositor
    Rosa Barrio, James Sutherland
    Article
    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:
    Insert
    SUMO1nc (SUMO1 Human)
    Use
    Lentiviral
    Tags
    Low-affinity Avitag
    Mutation
    Q94P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208042-plasmid-map-sequence-id-410263

    Enlarge

  16. TRIPZ-CTID195-GSQ-SUMO1nc-PURO

    Plasmid
    #208035
    Purpose
    Enables inducible expression of C-terminal Split-TurboID-fused SUMO1nc, to perform SUMO-ID; nc = non-cleavable mutation; selection with puromycin
    Depositor
    Rosa Barrio, James Sutherland
    Article
    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:
    Insert
    SUMO1nc (SUMO1 Human)
    Use
    Lentiviral
    Tags
    Myc, C-terminal Split-TurboID
    Mutation
    Q94P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208035-plasmid-map-sequence-id-410209

    Enlarge

  18. MZB184

    Plasmid
    #228488
    Purpose
    Empty 10xHis-SUMO (Smt3) vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression
    Depositor
    Richard Baker
    Type
    Empty backbone
    Tags
    10xHis-SUMO(Smt3)
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228488-plasmid-map-sequence-id-458447

    Enlarge

  19. MZB206

    Plasmid
    #228493
    Purpose
    Bacterial expression of 10xHis-SUMO (Smt3) fused Avi-tag, HRV-3C cleavable MSP2N2 for biotinylated nanodiscs
    Depositor
    Richard Baker
    Insert
    MSP2N2
    Tags
    10xHis-SUMO(Smt3)-Avitag-HRV
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228493-plasmid-map-sequence-id-455388

    Enlarge

  20. pHyperTEV60

    Plasmid
    #228496
    Purpose
    10xHis-SUMO (Smt3) tagged HyperTEV60 engineered protease (E. coli based protein expression)
    Depositor
    Richard Baker
    Insert
    TEV Protease
    Tags
    10xHis-SUMO(Smt3)
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228496-plasmid-map-sequence-id-455395

    Enlarge

Showing: 301 - 320 of 634 results