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p663-UBC-miniTurbo-V5-ADCK1_IDG-K

Plasmid

#158195

Purpose

Gateway destination clone of ADCK1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-ADCK1 (ADCK1 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-CAMKK1_IDG-K

Plasmid

#158169

Purpose

Gateway destination clone of CAMKK1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-CAMKK1 (CAMKK1 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-CDK7_IDG-K

Plasmid

#158194

Purpose

Gateway destination clone of CDK7 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-CDK7 (CDK7 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-CDK10_IDG-K

Plasmid

#158171

Purpose

Gateway destination clone of CDK10 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-CDK10 (CDK10 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-ERN2_IDG-K

Plasmid

#189905

Purpose

Gateway destination clone of ERN2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

ERN2 (ERN2 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-STK3_IDG-K

Plasmid

#189879

Purpose

Gateway destination clone of STK3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

STK3 (STK3 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-RIOK2_IDG-K

Plasmid

#170586

Purpose

Gateway destination clone of RIOK2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-RIOK2 (RIOK2 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-STK32A_IDG-K

Plasmid

#170608

Purpose

Gateway destination clone of STK32A (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-STK32A (STK32A Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-MARK4_IDG-K

Plasmid

#189884

Purpose

Gateway destination clone of MARK4 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

MARK4 (MARK4 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-MARK3_IDG-K

Plasmid

#170583

Purpose

Gateway destination clone of MARK3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-MARK3 (MARK3 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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p667-UBC-PKMYT1-V5-TurboID_IDG-K

Plasmid

#135247

Purpose

Gateway destination clone of PKMYT1 (human) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

PKMYT1-V5-TurboID (PKMYT1 Human)

Use

Lentiviral; Gateway destination

Tags

V5-TurboID

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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p667-UBC-MAP3K14-V5-miniTurbo_IDG-K

Plasmid

#189903

Purpose

Gateway destination clone of MAP3K14 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

MAP3K14 (MAP3K14 Human)

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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p667-UBC-AAK1-V5-miniTurbo_IDG-K

Plasmid

#135255

Purpose

Gateway destination clone of AAK1 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

AAK1-V5-miniTurbo (AAK1 Human)

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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[UBC][NRE Pum][eGFP]

Plasmid

#97192

Purpose

The wild-type Pum, fused to eGFP, and behind the ubiquitin promoter.

Depositor

Edward Boyden

Article

Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

Insert

wild type Pumilio homology domain

Use

Synthetic Biology

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

UBC (ubiquitin C)

Available since

Nov. 2, 2017

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-NEK9_IDG-K

Plasmid

#189873

Purpose

Gateway destination clone of NEK9 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

NEK9 (NEK9 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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KA1875_pGL4-UBCmin-luc2/Sox2_SRR2

Plasmid

#124198

Purpose

Luciferase reporter driven by the Sox2 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Sox2 enhancer

Use

Luciferase

Tags

Expression

Mutation

Promoter

UBCmin

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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p667-UBC-NUAK2-V5-miniTurbo_IDG-K

Plasmid

#189910

Purpose

Gateway destination clone of NUAK2 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

NUAK2 (NUAK2 Human)

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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KA1868_pGL4-UBCmin-luc2/Utf1-A'

Plasmid

#124194

Purpose

Luciferase reporter driven by the Utf1 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Utf1 enhancer

Use

Luciferase

Tags

Expression

Mutation

Promoter

UBCmin

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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psubCAG-WPRE-hVEGF-D-Strept

Plasmid

#119227

Purpose

Plasmid encodes Strep-tagged full-length (unprocessed) VEGF-D of human origin to be used for stimulation of lymphangiogenesis in mice in vivo.

Depositor

Kari Alitalo

Insert

VEGFD (VEGFD Human)

Use

AAV

Tags

Strept

Expression

Mutation

Promoter

CAG

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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p663-UBC-V5-BioID-STOP_IDG-K

Plasmid

#158163

Purpose

Gateway destination clone of BioID (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

V5-BioID

Use

Lentiviral; Gateway destination

Tags

V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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