We narrowed to 856 results for: V2

1. pJRH-1179 U6-reci Gag-Cas9 v2

   Plasmid

   #201914

   Purpose

   Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Insert

   Gag-Cas9 v2
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJRH-1180 U6-reci Gag-pol v2

   Plasmid

   #201915

   Purpose

   Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Insert

   Gag-pol
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CROPseq-multi-v2-palmitoyl-mTagBFP2-BsmBI_entry (pRW1509)

   Plasmid

   #225755

   Purpose

   Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with membrane-localized palmitoyl-mTagBFP2; v2 is compatible with T7 in vitro transcription detection

   Depositor

   Paul Blainey

   Article

   Walton et al bioRxiv 2024.03.17.585235

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. plenti-CAG-IKZF2-V2-FLAG-IRES-GFP

   Plasmid

   #69049

   Purpose

   lentiviral expression of IKZF2 variant 2 with FLAG tag

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   IKZF2 (IKZF2 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and IRES-GFP

   Expression

   Mammalian

   Mutation

   splice variant 2

   Promoter

   CAG

   Available Since

   Oct. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. plenti-UbcP-IKZF1-V2-3xHA-pGK-Pur

   Plasmid

   #69051

   Purpose

   lentiviral expression of IKZF1 with HA tag

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   IKZF1 (IKZF1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   splice variant 2

   Promoter

   UBC

   Available Since

   Nov. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. plenti-UbcP-IKZF2-V2-3xHA-pGK-Pur

   Plasmid

   #69052

   Purpose

   lentiviral expression of IKZF2 variant 2 with HA tag

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   IKZF2 (IKZF2 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   splice variant 2

   Promoter

   UBC

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. V2-MESA-35F-Tev-MS2-p65-HSF1

   Plasmid

   #84513

   Purpose

   MESA protease chain with V2-MESA ectodomain, 35 extracellular linkers, a flag tag, and Tev protease, MS2-P65-HSF1

   Depositor

   Joshua Leonard

   Article

   Schwarz et al Nat Chem Biol. 2016 Dec 12. doi: 10.1038

   Inserts

   V2-MESA-35F-Tev
   MS2-P65-HSF1
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. XZ032 CMV-TO-CCR6-V2-ADAR2dd(E488Q, T501A)

   Plasmid

   #233394

   Purpose

   CCR6-V2-ADAR2dd(E488Q, T501A) with a short 4 amino acid linker and V2 tail

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   CCR6-ADAR2dd
   

   Expression

   Mammalian

   Mutation

   E488Q, T501A on ADAR2dd

   Promoter

   CMV

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. RPS4X sgRNA-3 lentiCRISPR v2-Blast plasmid

   Plasmid

   #192233

   Purpose

   lentiviral vector expressing sgRNA-3 targeting eIF3 binding site of RPS4X 5'UTR

   Depositor

   Dieter Wolf

   Article

   Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

   Insert

   sgRNA-3 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. RPS15A sgRNA-5 lentiCRISPR v2-Blast plasmid

    Plasmid

    #192230

    Purpose

    lentiviral vector expressing sgRNA-5 targeting eIF3 binding site of RPS15A 5'UTR

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-5 targeting eIF3 binding site of RPS15A 5'UTR (RPS15A Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. RPS4X sgRNA-4 lentiCRISPR v2-Blast plasmid

    Plasmid

    #192234

    Purpose

    lentiviral vector expressing sgRNA-4 targeting eIF3 binding site of RPS4X 5'UTR

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-4 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. RPS15A sgRNA-4 lentiCRISPR v2-Blast plasmid

    Plasmid

    #192229

    Purpose

    lentiviral vector expressing sgRNA-4 targeting eIF3 binding site of RPS15A 5'UTR

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-4 targeting eIF3 binding site of RPS15A 5'UTR (RPS15A Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CMVTO-GFP-Kir2.1-HA-tevs-RXR-V2

    Plasmid

    #179705

    Purpose

    TEVP-inducible GFP-conjugated Kir2.1 RELEASE

    Depositor

    Xiaojing Gao

    Article

    Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

    Insert

    Kir2.1
    

    Expression

    Mammalian

    Available Since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CMVTO-mCherry-Kir2.1-HA-tvmvs-RXR-V2

    Plasmid

    #179707

    Purpose

    TVMVP-inducible mCherry-conjugated Kir2.1 RELEASE

    Depositor

    Xiaojing Gao

    Article

    Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

    Insert

    Kir2.1
    

    Expression

    Mammalian

    Available Since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKLV2-U6(gDmap1 v2)-PGK-Puro-BFP

    Plasmid

    #117141

    Purpose

    Express gRNA vs Dmap1

    Depositor

    Kosuke Yusa

    Article

    Yu et al Stem Cells. 2019 Apr 1. doi: 10.1002/ste

    Insert

    gDmap1 v2 (Dmap1 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Promoter

    human U6 promoter, mouse PGK promoter

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Fibronectin-human-mutated V2 O-Glycosylation site

    Plasmid

    #120405

    Purpose

    enables eukaryotic expression of human plasma fibronectin in which the second O-glycosylation site was mutated from threonine to serine

    Depositor

    Inaam Nakchbandi

    Article

    Sens et al J Bone Miner Res. 2017 Jan;32(1):70-81.

    Insert

    Fibronectin (FN1 Human)
    

    Expression

    Mammalian

    Mutation

    Contains complete variable region with a mutation…

    Promoter

    CMV

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJRH-1345 U6-B2M sgRNA Gag-Cas9 v2

    Plasmid

    #201916

    Purpose

    Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Inserts

    Gag-Cas9 v2
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    

    Expression

    Mammalian

    Promoter

    CAG and Human U6

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJRH-1346 U6-B2M sgRNA Gag-pol v2

    Plasmid

    #201917

    Purpose

    Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Inserts

    Gag-pol
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    

    Expression

    Mammalian

    Promoter

    CAG and Human U6

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CROPseq-multi-v2-SpCas9-P2A-Puro-BsmBI_entry (pRW1512)

    Plasmid

    #225756

    Purpose

    Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with expression of SpCas9 and Puromycin resistance; v2 is compatible with T7 in vitro transcription detection

    Depositor

    Paul Blainey

    Article

    Walton et al bioRxiv 2024.03.17.585235

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pZ P (cHS4)X4 OCT4p eGFP SV40pA v2

    Plasmid

    #115518

    Purpose

    Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows OCT4 dependent expression of GFP, and contains cHS4 insulators.

    Depositor

    Catherine Verfaillie

    Article

    Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-

    Insert

    eGFP
    

    Use

    AAV; Donor plasmid for recombinase-mediated casse…

    Expression

    Mammalian

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge