Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 1,450 results for: pUC57

Showing: 301 - 320 of 1450 results

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pUC57-mini v2-HF24_F2

Plasmid

#195716

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Publication

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#2 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-mini v2-HF24_F11

Plasmid

#195725

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Publication

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#11 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-mini v2-HF24_F16

Plasmid

#195730

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Publication

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#16 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-mini v2-HF24_F18

Plasmid

#195732

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Publication

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#18 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-mini v2-HF24_F1

Plasmid

#195715

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Publication

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#1 of a 24 fragment split in lacI/lacZ cassette

Use

Synthetic Biology

Available since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-mini v2-HF24_F9

Plasmid

#195723

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Publication

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#9 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-mini v2-HF24_F23

Plasmid

#195737

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Publication

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#23 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-mini v2-HF24_F21

Plasmid

#195735

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Publication

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#21 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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SRAcode_in_pUC57_v3(419-999)

Plasmid

#49824

Purpose

for transcribing SRA nucl 419-999 of NM_001035235 with T7

Depositor

Thomas Cech

Publication

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator RNA coding (SRA1 Human)

Expression

Bacterial

Mutation

KpnI site at 5' start of RNA coding seq

Promoter

T7

Available since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

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SRAcode_in_pUC57_v3(767-999)

Plasmid

#49825

Purpose

for transcribing SRA nucl 767-999 of NM_001035235 with T7

Depositor

Thomas Cech

Publication

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator RNA coding (SRA1 Human)

Expression

Bacterial

Mutation

KpnI site at 5' start of RNA coding seq

Promoter

T7

Available since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

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SRAcode_in_pUC57_v3(419-771)

Plasmid

#49823

Purpose

for transcribing SRA nucl 419-771 of NM_001035235 with T7

Depositor

Thomas Cech

Publication

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator RNA coding (SRA1 Human)

Expression

Bacterial

Mutation

KpnI site at 5' start of RNA coding seq

Promoter

T7

Available since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pUC57-Homology Arms-BRD4-HaloTag

Plasmid

#228575

Purpose

CRISPR donor plasmid to tag human BRD4 with Halo tag; for CRISPR-mediated gene insertion

Depositor

Marcin Imielinski

Insert

Homology Arms-BRD4-HaloTag (BRD4 Human)

Use

CRISPR

Expression

Mammalian

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pUC57 DYSF, alt exon 1a, 5a, 40a

Plasmid

#87897

Purpose

research of alternative isoforms of the protein dysferlin

Depositor

Jain Foundation

Insert

DYSF, alt exon 1a, 5a, 40a (DYSF Human)

Expression

Bacterial

Mutation

alternative isoforms, see comments

Available since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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pUC57-2xFLAG-mAID-AMBRA1 HDR donor template

Plasmid

#172609

Purpose

Donor plasmid containing the HDR template for the in-frame, N-terminal 2xFLAG-mAID tagging of AMBRA1 gene

Depositor

Michele Pagano

Publication

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

2xFLAG-mAID-AMBRA1 (AMBRA1 Synthetic, Human)

Use

CRISPR; Human targeting

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pUC57-FlpTag-GFP-ph0

Plasmid

#231897

Purpose

Vector with FlpTag-GFP, an inverted GFP enabling conditional tagging and visualization of endogenous proteins via Flp recombinase activity. Codon phase 0.

Depositor

Alexander Borst

Publication

Fendl et al Elife. 2020 Oct 20;9:e62953. doi: 10.755

Insert

SD-GFP-SA

Expression

Bacterial

Available since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pUC57-FlpTag-GFP-ph1

Plasmid

#231898

Purpose

Vector with FlpTag-GFP, an inverted GFP enabling conditional tagging and visualization of endogenous proteins via Flp recombinase activity. Codon phase 1.

Depositor

Alexander Borst

Publication

Fendl et al Elife. 2020 Oct 20;9:e62953. doi: 10.755

Insert

SD-GFP-SA

Expression

Bacterial

Available since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pUC57-FlpTag-GFP-ph2

Plasmid

#231899

Purpose

Vector with FlpTag-GFP, an inverted GFP enabling conditional tagging and visualization of endogenous proteins via Flp recombinase activity. Codon phase 2.

Depositor

Alexander Borst

Publication

Fendl et al Elife. 2020 Oct 20;9:e62953. doi: 10.755

Insert

SD-GFP-SA

Expression

Bacterial

Available since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pUC57 mini-FLAG-HA-Regnase-3 WT

Plasmid

#222651

Purpose

For in vitro transcription of mouse Zc3h12c tagged with FLAG-HA at N-term

Depositor

Silvia Monticelli

Publication

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c (Zc3h12c Mouse)

Use

In vitro transcription

Tags

FLAG and HA

Promoter

T7

Available since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-Peft-pHCountdown-unc54

Plasmid

#182304

Purpose

C. elegans plasmid encoding pH-Countdown with three introns

Depositor

Andrew York

Publication

Seidel et al (2021), doi: 10.5281/zenodo.5810930

Insert

pH countdown with 3 introns, with a Peft3 promoter and unc54 terminator

Expression

Worm

Promoter

Peft-3

Available since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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pUC57-attB2-SA(1)-CRY2-mCherry

Plasmid

#160440

Purpose

To create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 1.

Depositor

Nicholas Tolwinski

Publication

Bunnag et al J Mol Biol. 2020 May 1;432(10):3159-3176

Insert

Cryptochrome 2 (CRY2 Mustard Weed)

Tags

mCherry

Expression

Insect

Available since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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Showing: 301 - 320 of 1450 results

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