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Showing: 301 - 320 of 446 results

  1. LV TRE-GFP-TMD-DLL1-Nbox-mutant

    Plasmid
    #194937
    Purpose
    LV construct encoding a mutated GFP-TMD-DLL1 ligand containing an ablated Nbox site under dox-inducible TRE promoter and containing a PGK-rtTA-M2-IRES-Puro regulator/resistance marker cassette.
    Depositor
    John Ngo
    Article
    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103
    Insert
    eGFP-(PDGFR)TMD-rDLL1 ICD-Nbox-AlaX3-mutant
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    194937-plasmid-map-sequence-id-386567

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  2. PB-CT3G-CERP2-MG-IRES2-mNeonGreen

    Plasmid
    #175508
    Purpose
    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (CMVe-hEF1a-driven rtTA and puromycin resistance)
    Depositor
    George Church
    Article
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G-mCMV
    Available since
    Oct. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    175508-plasmid-map-sequence-id-345097

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  3. pTon1(acgfp1)

    Plasmid
    #233648
    Purpose
    Expression vector of AcGFP1 under control of tetracycline responsive element (TRE). Also express Tet-on advenced transactivator (rtTA) under control of minimal CMV promoter.
    Depositor
    Takeo Kubo
    Article
    Tsujioka et al Nat Commun. 2017 Sep 8;8(1):495. doi: 10
    Insert
    target sequence of guide RNA #tyr (3253-3275) 5'-GGCTCCATGTCTTCCGTCCAAGG-3'
    Use
    Amphibian expression, tet-on
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    233648-plasmid-map-sequence-id-468211

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  7. PB-CT3G-CERP2-MG-IRES2-mCherry

    Plasmid
    #175507
    Purpose
    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mCherry (CMVe-hEF1a-driven rtTA and puromycin resistance)
    Depositor
    George Church
    Article
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G-mCMV
    Available since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    175507-plasmid-map-sequence-id-345876

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  8. PB-CT3G-CERP2-MG-IRES2-mTagBFP2

    Plasmid
    #175509
    Purpose
    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mTagBFP2 (CMVe-hEF1a-driven rtTA and puromycin resistance)
    Depositor
    George Church
    Article
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G-mCMV
    Available since
    Oct. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    175509-plasmid-map-sequence-id-345099

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  9. PB-CT3G-ERP2-MG-IRES2-mNeonGreen

    Plasmid
    #175506
    Purpose
    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (hEF1a-driven rtTA and puromycin resistance)
    Depositor
    George Church
    Article
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G-mCMV
    Available since
    Oct. 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    175506-plasmid-map-sequence-id-345872

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  17. pLenti-TetO-PM

    Plasmid
    #197387
    Purpose
    To establish all-in-one TetO-inducible cell line for Pdx1-Mafa expression (Doxycycline induced; rtTA included; Blasticidin resistant)
    Depositor
    Qiao Zhou
    Article
    Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038
    Insert
    PDX1-T2A-MAFA
    Use
    Lentiviral; Teto
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE
    Available since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    197387-plasmid-map-sequence-id-385579

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  18. pATP416-­ptetO7-­pphlO6-­crtYBI

    Plasmid
    #165976
    Purpose
    Expresses carotenoid biosynthesis gene in response to 2,4-diacetylphloroglucinol and doxycycline in yeast expressing rtTA and PhlTA
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:
    Inserts
    Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
    Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
    BTS1 (BTS1 Budding Yeast)
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    C1281A, G1698A and C66A, C1359T
    Promoter
    Synthetic promoter (pphlO6), Synthetic promoter (…
    Available since
    March 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    165976-plasmid-map-sequence-id-328850

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  20. pATP416-­pluxO5-­ptetO7-­crtYBI

    Plasmid
    #165975
    Purpose
    Expresses carotenoid biosynthesis gene in response to homoserine lactone and doxycycline in yeast expressing LuxTA and rtTA
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:
    Inserts
    Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
    Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
    BTS1 (BTS1 Budding Yeast)
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    C1281A, G1698A and C66A, C1359T
    Promoter
    Synthetic promoter (pluxO5), Synthetic promoter (…
    Available since
    March 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    165975-plasmid-map-sequence-id-328841

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Showing: 301 - 320 of 446 results