We narrowed to 11 results for: rtta
-
TypeBlog Post...lentivirus for delivering control shRNAs, GFP, and rtTA from the Tet-on system. The first two can serve ...serve as controls in a variety of experiments, while rtTA can be used to activate expression of a gene under...
-
New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression
TypeBlog Post...transposase. In rtTA-cell lines, it is better to first engineer the cell line with rtTA, then integrate...can be used directly in transient transfection in rtTA+ cell lines (such as HEK293-TETOn cells), or they... -
Quick Guide to All Things Lentivirus
TypeBlog Post...pPRIME-Tet-GFP-FF3) and one with either tTA or rtTA (FUW-M2rtTA). In this kind of approach, a population of...Tet-on system, tTA has been modified (and renamed rtTA) to bind TRE only in presence of Dox. In this case...vector systems containing both your transgene and tTA/rtTA (pINDUCER20, pINDUCER21) have been developed, but... -
Tetracycline Inducible Expression
TypeCollection... Tet-On 3G rtTA Bong-Kiun Kaang 20342 FUW-M2rtTA Lentiviral Tet-On vector, expressing rtTA from UbC promoter...transactivator (rtTA) was created by fusing rTetR with VP16. In the absence of tetracycline, rtTA is unable ...tetracycline, rtTA binds the TRE, promoting gene expression. Tetracycline On is also known as the rtTA-dependent...promoter rtTA-Advanced Rudolf Jaenisch 25434 pMA2640 Retroviral Tet-On vector for CMV-driven rtTA with EGFP...175274 pAAV-rtTA AAV Tet-On vector with neuron-specific expression of rtTA3 from hSyn promoter rtTA3 Wei Xu...Advanced : improved transactivator (rtTA-Advanced, also known as rtTA2 S -M2) with greater sensitivity to...effects (Urlinger et al., 2000). rtTA3 and Tet-On 3G : further improved rtTA variants with lower background... -
Plasmids 101: Inducible Promoters
TypeBlog Post...direct activation. In this system, the activator rtTA (reverse tetracycline-controlled transactivator)... -
Easi-CRISPR: Generating Knock-In and Conditional Mouse Models
TypeBlog Post... and tetracycline drug induction systems (ttA or rtTA) are between 1 to 2 kb in length. There are plenty... -
Retrovirus Plasmids
TypeCollection...MSV Tet-regulated expression of shRNA; expresses rtTA. See Zuber et al. 2011 for additional plasmids in... -
Lentivirus Plasmids
TypeCollection...Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-puro. See article for more versions of this plasmid... -
22 Hot Plasmid Technologies from 2014
TypeBlog Post...cells stably expressing pTRE-Tight-MitoTimer and rtTA. The cells were pulsed with 2µg/ml of Doxycycline... -
Neurodegeneration Plasmid Collection
TypeCollection...GST-TEV-TBK1 TBK1 polH ALS Sascha Martens 209078 piggyBac-rtTA (4th_Gen)-SNCA(3K)-sfGFP-IRES-NGN2-puro (VK_1120...GFP TRE Parkinson's Vikram Khurana 209080 piggyBac-rtTA (4th_Gen)-SNCA(A53T)-sfGFP-IRES-NGN2-puro (VK_1123...GFP TRE Parkinson's Vikram Khurana 209081 piggyBac-rtTA (4th_Gen)-SNCA(A53T-DNAC)-sfGFP-IRES-NGN2-puro (...Episodic ataxia Giulio Superti-Furga 132389 AAVS1 CAG rtTA3 TauWT 2N4R-EGFP MAPT GFP TREtight Parkinson's, FTD... FTD Gerold Schmitt-Ulms 132393 AAVS1 CAG rtTA3 TauP301L 2N4R-EGFP MAPT GFP TREtight Parkinson's, FTD ...SNCA Avi CMV Parkinson's Vikram Khurana 209085 piggyBac_rtTA (4th_Gen)_SNCA_IRES_NGN2-2A-PURO_Synapsin-BirA... SNCA TRE Parkinson's Vikram Khurana 209086 piggyBac_rtTA (4th_Gen)_SNCA-Avi_IRES_NGN2-2A-PURO_Synapsin-BirA... -
Cre-lox system
TypeCollection...EGFP- Cre fusion CAG Mammalian Stringer 68448 TOPO rtTA3-2A-Cre iRFP670, Cre, and GFP EFS Lentiviral Jacks...