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pEIF3D-WT-sgNT

Plasmid

#222120

Purpose

EIF3D overexpression vector with non-targeting sgRNA

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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pEIF3D-RNAgateDel-sgEIF3D

Plasmid

#222137

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

V291-F306 deletion

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEIF3D-NtermDel-sgEIF3D

Plasmid

#222123

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

S161 truncation

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCon-IFl_Con-IEr

Plasmid

#192210

Purpose

Consensus I-F CRISPR Leader and a CRISPR locus with two consensus I-E repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-F Leader and Consensus I-E Locus DNA

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IFl_Con-ICr

Plasmid

#192211

Purpose

Consensus I-F CRISPR Leader and a CRISPR locus with two consensus I-C repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-F Leader and Consensus I-C Locus DNA

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IFl_Con-IIAr

Plasmid

#192212

Purpose

Consensus I-F CRISPR Leader and a CRISPR locus with two consensus II-A repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-F Leader and Consensus II-A Locus DNA

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IEl_Con-IEr

Plasmid

#192213

Purpose

Consensus I-E CRISPR Leader and a CRISPR locus with two consensus I-E repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-E Leader and Consensus I-E Locus DNA

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-ICl_Con-ICr

Plasmid

#192214

Purpose

Consensus I-C CRISPR Leader and a CRISPR locus with two consensus I-C repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-C Leader and Consensus I-C Locus DNA

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IIAl_Con-IIAr

Plasmid

#192215

Purpose

Consensus II-A CRISPR Leader and a CRISPR locus with two consensus II-A repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus II-A Leader and Consensus II-A Locus DNA

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pX1-IFl_Con-IFr

Plasmid

#192216

Purpose

Mutant I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

I-F leader and I-F repeat construct not studied further in this paper (X1)

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pX2-IFl_Con-IFr

Plasmid

#192217

Purpose

Mutant I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

I-F leader and I-F repeat construct not studied further in this paper (X2)

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEIF3D-a11helixDel-sgEIF3D

Plasmid

#222135

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

T317E, N320E, H321A

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEIF3D-CtermDel-sgEIF3D

Plasmid

#222125

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

F527 truncation

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEIF3D-a5helixDel-sgEIF3D

Plasmid

#222133

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

D249Q, V262I, Y263A

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Joey1

Plasmid

#217221

Purpose

Mammalian expression vector for expression of Joey1 transposase (CMV promoter)

Depositor

Yong E Zhang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

Joey1 transposase

Expression

Mammalian

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE-MmCENP-O-GFP

Plasmid

#205178

Purpose

Expresses mouse CENP-O C-term tagged with GFP under T7 promoter - designed for in vitro transcription

Depositor

Michael Lampson

Article

Dudka et al J Cell Biol. 2023 Sep 4;222(9):e20221208

Insert

CENP-O (Cenpo Mouse)

Use

In vitro transcription

Tags

5 glycines linker followed by GFP

Expression

Mammalian

Mutation

Wild type mouse CENP-O

Promoter

T7

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF2722

Plasmid

#144221

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZNF70 (ZNF70 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF0939

Plasmid

#142212

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZNF473 (ZNF473 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pVE2_1_p40S_QfDBD_VP16AD_cp_pcbC_DsRed_1xQUAS

Plasmid

#154221

Purpose

Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 1xQUAS+CP_pcbC

Depositor

Arnold Driessen

Article

Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.

Insert

p40S:QfDBD-VP16AD-GFP-NLS

Use

Synthetic Biology; Fungal expression

Tags

GFP-NLS; DsRed-SKL

Expression

Bacterial

Promoter

p40S (AN0465 A. nidulans)

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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rat brain inositol 1,4,5-trisphosphate 3-kinase A

Plasmid

#122155

Purpose

rat brain cDNA encoding a Ca2+/calmodulin-sensitive inositol 1,4,5-trisphosphate 3-kinase A; clone CP16 (full length)

Depositor

Christophe Erneux

Article

Takazawa et al Biochem J. 1990 Nov 15;272(1):107-12.

Insert

inositol 1,4,5-trisphosphate 3-kinase A (Itpka Rat)

Expression

Bacterial

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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