We narrowed to 9,934 results for: SEC

1. pAAV-TRE-DIO-eGFP-2A-TetanusToxin

   Plasmid

   #166603

   Purpose

   Encodes Cre-dependent eGFP-2A tetanus toxin light chain fragment under control of the TRE

   Depositor

   Martin Myers

   Article

   Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

   Insert

   EGFP-2A-Tetanus Toxin
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   tetracycline response element

   Available since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTriEx4-Cdc42-2G

   Plasmid

   #68814

   Purpose

   FRET-based biosensor reporting on endogenous Cdc42 activation (for expression in E. coli, insect and vertebrate cells)

   Depositor

   Olivier Pertz

   Article

   Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

   Insert

   Cdc42 activation reporter
   

   Use

   Tags

   His and Myc

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLIB-HIS-TEV-scCAK1

   Plasmid

   #177014

   Purpose

   Generate baculovirus for insect cell expression of scCAK1 with N-terminal Polyhistidine-TEV

   Depositor

   Andrea Musacchio

   Article

   Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

   Insert

   CAK1 cyclin-dependent protein kinase-activating kinase CAK1 (CAK1 Budding Yeast)
   

   Use

   Tags

   Polyhistidine-TEV

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Str-KDEL_ST-SBP-EGFP

   Plasmid

   #65264

   Purpose

   synchronize trafficking of ST from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)
   

   Use

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 43 of ST

   Promoter

   CMV

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. Str-KDEL_TNF-SBP-EGFP

   Plasmid

   #65278

   Purpose

   synchronize trafficking of TNF from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   TNF (TNF Human)
   

   Use

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEV53D

   Plasmid

   #44168

   Depositor

   John Olsen

   Article

   Patel et al J Gene Med. 2013 Jan;15(1):51-62. doi: 1

   Insert

   Equine infectious anemia virus (EIAV) gag-pol,rev, and S2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   A131T mutation in gag; Deletion of viral LTR sequ…

   Promoter

   CMV

   Available since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-ACE2-GFP

   Plasmid

   #154962

   Purpose

   pcDNA3.1-ACE2-GFP

   Depositor

   Utpal Pajvani

   Article

   Bartolome et al Sci Rep. 2021 May 7;11(1):9803. doi: 10.

   Insert

   ACE2-GFP (ACE2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Str-KDEL_TNF-SBP-mCherry

   Plasmid

   #65279

   Purpose

   synchronize trafficking of TNF from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   TNF (TNF Human)
   

   Use

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-pEF1a-EBFP-tTAn

   Plasmid

   #172122

   Purpose

   Constitutive expression of EBFP and rtTA-InteinN

   Depositor

   Chun Xu

   Article

   Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

   Insert

   EBFP, tTAn-InteinN
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVDL9.3

    Plasmid

    #168299

    Purpose

    Secretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyA

    Depositor

    Víctor de Lorenzo

    Article

    Tzschaschel et al Nat Biotechnol. 1996 Jun;14(6):765-9. do

    Insert

    Segment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    LacI-Plac

    Available since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDR111_Bgal no NLS

    Plasmid

    #188397

    Purpose

    Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.

    Depositor

    Christopher Contag

    Article

    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

    Insert

    lacZ
    

    Use

    Tags

    PhoD secretion peptide

    Expression

    Bacterial

    Mutation

    Promoter

    Phyper spank

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti EF1a GFP-mCherry-mPlin1

    Plasmid

    #189005

    Purpose

    Fluorescent reportor for lipophagy

    Depositor

    Atsushi Hoshino

    Article

    Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

    Insert

    GFP, mChery, Plin1 (Plin1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. CIB81-pmGFP

    Plasmid

    #75366

    Purpose

    Expresses aa1-81 of Arabidopsis CIB1 fused to EGFP with CaaX membrane tag on end

    Depositor

    Chandra Tucker

    Article

    Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

    Insert

    CIB1 (CIB1 Mustard Weed)
    

    Use

    Tags

    prenylated EGFP

    Expression

    Mammalian

    Mutation

    residues 1-81 of CIB1

    Promoter

    CMV

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. Arf Q71L CFP

    Plasmid

    #128149

    Purpose

    Expression of Arf Q71L

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Presley et al Nature. 2002 May 9;417(6885):187-93. doi

    Insert

    Arf1 (ARF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q71L

    Promoter

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-28a-His6-SUMO-Munc18-1wt

    Plasmid

    #135550

    Purpose

    Express rat Munc18-1 in E. coli BL21, purified via HisPur Ni-NTA Resin

    Depositor

    Jingshi Shen

    Article

    Yu et al Methods Mol Biol. 2019;1860:237-249. doi

    Insert

    Munc18-1wt
    

    Use

    Tags

    6xHis tag, thrombin recognition and cleavage site…

    Expression

    Bacterial

    Mutation

    No ATG start codon

    Promoter

    T7 promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. SAE1

    Plasmid

    #175241

    Purpose

    Bacterial expression and purification, combined with SAE2 to form the E1 enzyme heterodimer

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    SAE1 (SAE1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. SAE2-mGarnet

    Plasmid

    #175240

    Purpose

    Bacterial expression and purification, combined with SAE1 to form the E1 enzyme heterodimer

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    UBA2 (UBA2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3 GATA1

    Plasmid

    #85693

    Purpose

    Mammalian expression of mouse GATA1

    Depositor

    Giannino Del Sal, Licio Collavin

    Article

    Collavin et al Proc Natl Acad Sci U S A. 2004 Jun 15;10

    Insert

    GATA1 (Gata1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pACEBac1-PARP2-Flag-His-WT

    Plasmid

    #111574

    Purpose

    Expresses full length PARP2 in Sf9 (insect) cells

    Depositor

    Thomas Muir

    Article

    Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

    Insert

    PARP2 (PARP2 Human)
    

    Use

    Tags

    C-terminal 6xHis and C-terminal Flag

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin promoter

    Available since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. mH2A1.1-CT-GFP

    Plasmid

    #45167

    Depositor

    Brian Chadwick, Hunt Willard

    Article

    Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

    Insert

    H2Afy (MACROH2A1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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