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AiP1049-pAAV-mscRE13-minBGpromoter-tTA2-WPRE-hGHpA

Plasmid

#163482

Purpose

Direct-expressing tTA2 AAV Virus. Alias: AiP1049 - pAAV-AiE2013m-minBG-tTA2-WPRE-HGHpA

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

tTA2

Use

AAV

Promoter

Beta Globin minimal promoter

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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Human/mouse ELL2

Plasmid

#127254

Purpose

Expresses a human/mouse chimera that increases ELL2 expression in mammalian cells

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

N-terminal human/C-terminal mouse ELL2 (ELL2, Ell2 Human, Mouse)

Use

Other

Tags

His6X

Expression

Mammalian

Mutation

N terminal amino acids (1-287) are human, C termi…

Promoter

pEF1a

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pMIR-Report-RASA1-3'UTR (mut - miR-206 site)

Plasmid

#62576

Purpose

Translational Luciferase Reporter containing the mutated 3'UTR of RASA1. The miR-206 binding site was mutated.

Depositor

Michael Ruppert

Article

Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

Insert

RASA1 (RASA1 Human)

Use

Luciferase

Tags

None

Mutation

miR-206 binding site mutated (ACATTCCA --> AAC…

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pBABEpuro-ERBB2 C299S

Plasmid

#40988

Depositor

Matthew Meyerson

Article

Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

Insert

ERBB2 (ERBB2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

C299S

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only
pDONR223-GK2

Plasmid

#23827

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

GK2 (GK2 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-CDKL4

Plasmid

#23382

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CDKL4 (CDKL4 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-DGKB

Plasmid

#23477

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DGKB (DGKB Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-GK5

Plasmid

#23554

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

GK5 (GK5 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-DCAKD

Plasmid

#23585

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DCAKD (DCAKD Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-GLYCTK

Plasmid

#23653

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

GLYCTK (GLYCTK Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
NYESO beta/alpha into TRAC HDRT Source (pTR 169)

Plasmid

#112021

Purpose

DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-alpha

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

NYESO beta/alpha into TRAC HDRT

Use

CRISPR and Synthetic Biology

Available Since

Sept. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223-ERBB2

Plasmid

#23888

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ERBB2 (ERBB2 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
AiP1010-pAAV-mscRE4-minBGpromoter-iCre-WPRE-hGHpA

Plasmid

#163476

Purpose

Direct-expressing iCre AAV Virus. Alias: AiP1010 - pAAV-AiE2004m-minBG-iCre-WPRE-HGHpA

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

iCre

Use

AAV

Promoter

Beta Globin minimal promoter

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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AiP11818-pAAV-AiE2004m_3xC2-minCMV-SYFP2-WPRE3-BGHpA (Alias: CN1818)

Plasmid

#164458

Purpose

AiE2004m_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated transgene expression in L4/5_IT cortical neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

SYFP2

Use

AAV

Promoter

minCMV

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-LRPPRC

Plasmid

#23521

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

LRPPRC (LRPPRC Human)

Use

Gateway donor vector

Mutation

G246A; deleted E579

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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NYESO alpha/beta into TRBC1 HDRT Source (pTR 262)

Plasmid

#112022

Purpose

DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-beta

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

NYESO alpha/beta into TRBC1 HDRDT

Use

CRISPR and Synthetic Biology

Available Since

Nov. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223-MAGI1

Plasmid

#23523

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAGI1 (MAGI1 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-ERBB3

Plasmid

#23874

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ERBB3 (ERBB3 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-ABL1

Plasmid

#23939

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ABL1 (ABL1 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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Ai139 (TIT2L-GFP-ICL-TPT) targeting vector

Plasmid

#114426

Purpose

Target a Cre-dependent GFP and a tdTomato-P2A-tTA2 expression cassette into the mouse TIGRE locus

Depositor

Hongkui Zeng

Article

Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

Insert

GFP, tdTomato, tTA2,

Use

Cre/Lox and Mouse Targeting

Promoter

TRE2, CAG

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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