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Addgene

We narrowed to 13,212 results for: 109

Showing: 8051 - 8100 of 13212 results
  1. RUBYm2

    Plasmid
    #232175
    Purpose
    Introduce a C-to-T mutation to generate an early stop codon (Q139 to stop), thereby interrupting RUBY expression
    Depositor
    Insert
    RUBY mutant2 and CaMV 35S promoter
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
  2. VN-dCasE-VC-GK

    Plasmid
    #164277
    Purpose
    Express VN-dCasE-VC protein in mammalian cells. This plasmid is also referred to as as VN-dEcCas6-VC-GK in the publication.
    Depositor
    Insert
    VN-dCasE-VC
    Use
    Tags
    Expression
    Mammalian
    Mutation
    H20A in EcCasE
    Promoter
    CMV
    Available since
    July 29, 2025
    Availability
    Academic Institutions and Nonprofits only
  3. POC1426

    Plasmid
    #221573
    Purpose
    Plasmid containing the Insert 1 to be assembled in POC1430 using the site-selective methylation protection approach
    Insert
    DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  4. POC1427

    Plasmid
    #221574
    Purpose
    Plasmid containing the Insert 2 to be assembled in POC1430 using the site-selective methylation protection approach
    Insert
    DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  5. POC1428

    Plasmid
    #221575
    Purpose
    Plasmid containing the Insert 3 to be assembled in POC1430 using the site-selective methylation protection approach
    Insert
    DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  6. POC1429

    Plasmid
    #221576
    Purpose
    Plasmid containing the Insert 4 to be assembled in POC1430 using the site-selective methylation protection approach
    Insert
    DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  7. pYPQAT01

    Plasmid
    #223373
    Purpose
    T-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.
    Depositor
    Insert
    AtUBQ10-SpCas9-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  8. pYPQAT02

    Plasmid
    #223374
    Purpose
    T-DNA vector for SpCas9 mediated mutagenesis for plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.
    Depositor
    Insert
    ZmUbi-SpCas9-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  9. pYPQAT04

    Plasmid
    #223376
    Purpose
    T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.
    Depositor
    Insert
    ZmUbi-SpCas9-OsU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  10. pRC065d

    Plasmid
    #225979
    Purpose
    dRxCas13d expressed from J23107 promoter with J23119-driven crRNA (non-cognate DR)
    Depositor
    Inserts
    dRxCas13d
    buj1 crRNA (non-cognate DR) targets the unstructured RBS
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    J23107 and J23119
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
Showing: 8051 - 8100 of 13212 results