We narrowed to 8,419 results for: BLI;

1. pCer-C3-Plk1-T210D

   Plasmid

   #68133

   Purpose

   Expression of Cerulean-tagged, constitutively active Plk1 in mammalian cells

   Depositor

   Catherine Lindon

   Insert

   Polo-like kinase 1 (PLK1 Human)
   

   Use

   Tags

   Cerulean

   Expression

   Mammalian

   Mutation

   T210D constitutively active version

   Promoter

   CMV

   Available since

   Oct. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. p87AAVEGFP hp47phox (S303/304/328D)

   Plasmid

   #112250

   Purpose

   mutant p47phox

   Depositor

   Simone Di Giovanni

   Insert

   p47phox (NCF1 Human)
   

   Use

   AAV

   Tags

   EGFP

   Expression

   Mutation

   S303/304/328D

   Promoter

   CMV

   Available since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Hsp68-LacZ-Gibson

   Plasmid

   #170102

   Purpose

   Transgenic LacZ reporter construct for validation of enhancer sequences (inserted via Gibson-cloning). Can be introduced into the mouse genome via random integration following pro-nuclear injection.

   Depositor

   Len Pennacchio

   Type

   Empty backbone

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Mouse Hsp68 minimal promoter

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. m.5147-Left TALE-G1397-N-DddA11-mCherry

   Plasmid

   #211338

   Purpose

   Expression of codon-optimized mitochondrial base editor in human cells; cell sorting; blasticidin drug selection

   Depositor

   Monkol Lek

   Insert

   m.5147-Left TALE-G1397-N-DddA11-mCherry
   

   Use

   TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJK234

   Plasmid

   #73324

   Purpose

   Produces Janthinobacterium lividum violacein biosynthesis proteins VioA, VioB, and VioC

   Depositor

   T. J. Kappock

   Inserts

   violacein biosynthesis protein VioA
   violacein biosynthesis protein VioB
   violacein biosynthesis protein VioC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 and n/a

   Available since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCer-C3-Plk1-K82R

   Plasmid

   #68134

   Purpose

   Expression of Cerulean-tagged, kinase-dead Plk1 in mammalian cells

   Depositor

   Catherine Lindon

   Insert

   Polo-like kinase 1 (PLK1 Human)
   

   Use

   Tags

   Cerulean

   Expression

   Mammalian

   Mutation

   K82R kinase-dead version

   Promoter

   CMV

   Available since

   Oct. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSG5-Crkl mut SH2

   Plasmid

   #41703

   Depositor

   Nora Heisterkamp

   Insert

   Crkl (CRKL Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R39K

   Promoter

   SV40

   Available since

   April 9, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pShuttle-CMV-F2A-T2A-Venus

   Plasmid

   #62621

   Purpose

   AdEasy Shuttle vector modified for multicistronic expression. Contains T2A, F2A and Venus tag

   Depositor

   Zheng-Xu Wang

   Insert

   MCS with F2A and T2A
   

   Use

   Adenoviral

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. PRDX4

   Plasmid

   #38851

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   PRDX4 (Prdx4 Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pAAV-CaMKIIa-HA-rM3D(Gs)-IRES-mCitrine

    Plasmid

    #50468

    Purpose

    Gs-coupled rM3D-IRES-mCitrine under the control of CaMKIIa promoter

    Depositor

    Bryan Roth

    Insert

    rM3D-IRES-mCitrine
    

    Use

    AAV

    Tags

    HA

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    CaMKIIa

    Available since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSL1180-HR-PUbECFP

    Plasmid

    #47917

    Purpose

    Donor plasmid for homologous recombination that expresses ECFP consitutively using the Aedes aegypti polyubiquitin (PUb) promoter.

    Depositor

    Leslie Vosshall

    Insert

    ECFP
    

    Use

    Tags

    NLS

    Expression

    Insect

    Mutation

    Promoter

    Aedes aegypti polyubiquitin (PUb)

    Available since

    Sept. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. 371-GFP

    Plasmid

    #97030

    Purpose

    His6-GFP-tev-yORF

    Depositor

    Chris Jeans

    Type

    Empty backbone

    Use

    Tags

    GFP fusion, His6 tag, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUS252y

    Plasmid

    #191834

    Purpose

    Expresses fuYFP constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuYFP to place in other plasmid backbones.

    Depositor

    Nicholas Coleman

    Insert

    fuYFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 17, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pCas9CR5-NG

    Plasmid

    #120228

    Purpose

    pCas9CR5 with mutations to change PAM site specificity to NG from Nishimasu et al.

    Depositor

    Christopher Reisch

    Insert

    SpCas9-NG
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…

    Promoter

    pTet

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pet15b_ack

    Plasmid

    #220229

    Purpose

    Bacterial expression of AcK in E. coli BL21_DE3 (pLys) or similar

    Depositor

    Bernd Nidetzky

    Insert

    Acetate kinase (ackA )
    

    Use

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBS L30 mRuby3-Rab8A

    Plasmid

    #166428

    Purpose

    Rab8A fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.

    Depositor

    Raphael Gaudin

    Insert

    Rab8A
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    L30 weak promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1_C-6His-Stx17

    Plasmid

    #112259

    Purpose

    C-term His tagged pcDNA3.1 vector expressing mouse Stx17 (syntaxin 17)

    Depositor

    Madhulika Tripathi

    Insert

    syntaxin 17 (Stx17 Mouse)
    

    Use

    Tags

    6His Tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB CAG-L-bpA-2xSV40pA-L-tdGFP-Lrig1_shRNAmir_3

    Plasmid

    #178049

    Purpose

    CAG tdGFP Lrig1 shRNAmir-3 cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)

    Depositor

    Mario Capecchi

    Insert

    Lrig1 shRNAmir
    

    Use

    Cre/Lox and RNAi; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB CAG-L-bpA-2xSV40pA-L-tdGFP-Lrig1_shRNAmir_2

    Plasmid

    #178048

    Purpose

    CAG tdGFP Lrig1 shRNAmir-2 cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)

    Depositor

    Mario Capecchi

    Insert

    Lrig1 shRNAmir
    

    Use

    Cre/Lox and RNAi; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. PB CAG-L-bpA-2xSV40pA-L-tdGFP-Lrig1_shRNAmir_1

    Plasmid

    #178047

    Purpose

    CAG tdGFP Lrig1 shRNAmir-1 cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)

    Depositor

    Mario Capecchi

    Insert

    Lrig1 shRNAmir
    

    Use

    Cre/Lox and RNAi; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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