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pCDF-NtXNtTcR1AtR2NtB2

Plasmid

#160889

Purpose

Expression of N. tabacum Rubisco small subunit (rbcS-Tc), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

PT7-Nt-rbcS-Tc

Expression

Bacterial

Promoter

rbcS-Tc: T7, chaperones: T12

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCDF-NtXR1AtR2B2NtS1

Plasmid

#160879

Purpose

Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1 and A. thaliana Rubisco chaperone proteins raf2, and bsd2 in E. coli.

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

rbcS-S1, rbcX, raf1, raf2, bsd2

Expression

Bacterial

Promoter

rbcS-S1: pBAD, chaperones: T7

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pFUGW-RapACR_T111C-EYFP

Plasmid

#119195

Purpose

Fast anion channelrhodopsin mutant RapACR_T111C for time-resolved inhibition of neuronal spiking

Depositor

John Spudich

Article

Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

Insert

Anion channelrhodopsin RapACR fast mutant T111C

Use

Lentiviral

Tags

EYFP

Mutation

changed threonine 111 to cysteine

Promoter

Ubiquitin

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCDF-NtXNtT2R1AtR2NtB2

Plasmid

#160886

Purpose

Expression of N. tabacum Rubisco small subunit (rbcS-T2), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

PT7-Nt-rbcS-T2

Expression

Bacterial

Promoter

rbcS-T2: T7, chaperones: T9

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP167-3G124-HilA

Plasmid

#153322

Purpose

Triggers secretion of SptP167 fused to the 3G124nc anti-GFP DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP167-3G124 DARPin

Use

Salmonella expression

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP120-TD-HilA

Plasmid

#153330

Purpose

Triggers secretion of SptP120 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-HA4-7c12 Tandem Monobody

Use

Salmonella expression

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf137

Plasmid

#12825

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf137

Expression

Mammalian

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pSUPER.retro.puro-HCF-1-D

Plasmid

#36997

Depositor

Debu Chakravarti

Article

Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

Insert

HCF-1 shRNA (HCFC1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 21, 2012

Availability

Academic Institutions and Nonprofits only
pCASP-SptP120-E3_5-HilA

Plasmid

#153326

Purpose

Triggers secretion of SptP120 fused to the E3_5 DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-E3_5 DARPin

Use

Salmonella expression

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pJL1-TTlight-4xDQNAT

Plasmid

#128394

Purpose

In vitro expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Tetanus toxin light chain domain

Tags

4xDQNAT glycosylation tag and 6xHis tag

Expression

Bacterial

Mutation

Inactivating E234A mutation

Promoter

T7

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pSUPER.retro.puro-HCF-1-B

Plasmid

#36996

Depositor

Debu Chakravarti

Article

Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

Insert

HCF-1 shRNA (HCFC1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 15, 2012

Availability

Academic Institutions and Nonprofits only
pCASP-SptP120-K27-HilA

Plasmid

#153328

Purpose

Triggers secretion of SptP120 fused to the K27 DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-K27 DARPin

Use

Salmonella expression

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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ZJOM12

Plasmid

#133638

Purpose

Integration of GFP-TLG2 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast late golgi/early endosome.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

pCUP1-GFP-Tlg2 (TLG2 Budding Yeast)

Tags

EGFP

Expression

Yeast

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf221

Plasmid

#12909

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf221

Expression

Mammalian

Available Since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
pTH33 [pmyo-3::YFP-BeCyclOp(A-2x)::SL2::mCherry]

Plasmid

#168169

Purpose

Expression of YFP-BeCyclOp(A-2x) in BWMs of C. elegans

Depositor

Alexander Gottschalk

Article

Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

Insert

YFP-BeCyclOp(A-2x) (codon optimized for C. elegans)

Tags

YFP

Expression

Worm

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCR1277

Plasmid

#111101

Purpose

Expresses RAD51D_-_33446794.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

RAD51D gRNA (RAD51D Human)

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCDF-NtXNtT5R1AtR2NtB2

Plasmid

#160888

Purpose

Expression of N. tabacum Rubisco small subunit (rbcS-T5), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

PT7-Nt-rbcS-T5

Expression

Bacterial

Promoter

rbcS-T5: T7, chaperones: T11

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCDF-NtXNtT1R1AtR2NtB2

Plasmid

#160885

Purpose

Expression of N. tabacum Rubisco small subunit (rbcS-T1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

PT7-Nt-rbcS-T1

Expression

Bacterial

Promoter

rbcS-T1: T7, chaperones: T8

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCDF-NtXNtS5R1AtR2NtB2

Plasmid

#160884

Purpose

Expression of N. tabacum Rubisco small subunit (rbcS-S5), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

PT7-Nt-rbcS-S5

Expression

Bacterial

Promoter

rbcS-S5: T7, chaperones: T7

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCDF-NtXNtS2R1AtR2NtB2

Plasmid

#160883

Purpose

Expression of N. tabacum Rubisco small subunit (rbcS-S2), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

rbcS-S2, rbcX, raf1, raf2, bsd2

Expression

Bacterial

Promoter

rbcS-S2: T7, chaperones: T7

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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