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pAS_4xG1 PylT FLAG-G1 PylRS Y125A

Plasmid

#154769

Purpose

plasmid with 4xG1 PylT cassette and G1 PylRS Y125A for amber suppression in transient or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Publication

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)

Tags

FLAG

Expression

Mammalian

Mutation

Y125A

Promoter

EF1

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pSicoR-Ef1a-mCh-Puro-GFPi

Plasmid

#31848

Depositor

Bruce Conklin

Publication

Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

Insert

GFP RNAi

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6 for shRNA; Ef1alpha for mCherry-puromycin

Available since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pJZ012_EFS-Cas12a2-puro

Plasmid

#257716

Purpose

Express SuCas12a2 in mammalian cell lines; Puromycin selection.

Depositor

Jennifer Doudna

Publication

Zeng et al Nature. 2026 Jun 8. doi: 10.1038/s41586-

Insert

SuCas12a2

Use

CRISPR

Tags

NLS-FLAG

Expression

Mammalian

Promoter

EF-1alpha

Available since

July 7, 2026

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry_P2AT2A_GFP-nb-2xKS

Plasmid

#238236

Purpose

For overexpression of mCherry_P2AT2A_GFP-nb-2xKS

Depositor

Denes Hnisz

Publication

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry_P2AT2A_GFP-nb-2xKS

Expression

Mammalian

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCherryGFP-CoV2-WT

Plasmid

#177618

Purpose

Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting

Depositor

Junjie Guo

Publication

Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

Insert

SARS-CoV-2 FSE

Use

Lentiviral

Expression

Mammalian

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAW212

Plasmid

#172196

Purpose

P1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution with different restriction sites

Depositor

Chang Liu

Publication

Wellner et al Nat Chem Biol. 2021 Jun 24. pii: 10.1038

Insert

p10B2-AT110

Expression

Yeast

Promoter

10B2 (P1 specific promoter)

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-NF186WT-HA

Plasmid

#239774

Purpose

Expresses WT rat NF186 with a C-terminal HA tag in mammalian cells.

Depositor

Ivana Nikić-Spiegel

Publication

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Promoter

CMV

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pNP561

Plasmid

#222723

Purpose

Target and Recombinase Golden Gate Landing Pad Backbone for LT-RT with T7 Recombinase

Depositor

Patrick Hsu

Publication

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Type

Empty backbone

Tags

6xHis

Expression

Bacterial

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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Diffusive 27kDa (EGFP)

Plasmid

#201362

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Publication

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

EGFP

Expression

Mammalian

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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L_NLS 67kDa

Plasmid

#201361

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Publication

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40A4-EGFP-6PrA

Expression

Mammalian

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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H_NES 41kDa

Plasmid

#201346

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Publication

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

HIV_NES-EGFP-2PrA

Expression

Mammalian

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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M_NLS 41kDa

Plasmid

#201341

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Publication

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40A5-EGFP-2PrA

Expression

Mammalian

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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SRSF8_RRM_pGEX

Plasmid

#135119

Purpose

Recombinant expression of SRSF8 with dual-affinity tag

Depositor

Christopher Burge

Publication

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

SRSF8 (SRSF8 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pCAX-mScarlet-FBP17

Plasmid

#178366

Purpose

Expresses FBP17 c-terminal labeled with mScarelt in mammalian cells

Depositor

Erik Dent

Publication

Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

Insert

Formin Binding Protein 17 (FNBP1 Human)

Expression

Mammalian

Mutation

400 Glutamic Acid changed to Aspartic acid (403 A…

Promoter

Chicken Beta Actin

Available since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pCAX-mScarlet-CIP4

Plasmid

#178364

Purpose

Expresses CIP4 c-terminal labeled with mScarlet in mammalian cells

Depositor

Erik Dent

Publication

Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

Insert

Cdc42 Interacting Protein 4 (TRIP10 Human)

Expression

Mammalian

Promoter

CAG

Available since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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RBFOX3_RRM_pGEX

Plasmid

#135131

Purpose

Recombinant expression of RBFOX3 with dual-affinity tag

Depositor

Christopher Burge

Publication

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

RBFOX3 (RBFOX3 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available since

March 5, 2021

Availability

Academic Institutions and Nonprofits only

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pMG023 MBP-Axin 384-518-His (miniAxin)

Plasmid

#154012

Purpose

Expresses MBP-Axin_384-518-His (human Axin minimal scaffold fragment as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Publication

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

Axin1 (AXIN1 Human)

Tags

His6 and MBP

Expression

Bacterial

Mutation

381-518 fragment

Promoter

Tac

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pPD_Pinx-1::ChR2(H134R)::mCherry

Plasmid

#117420

Purpose

For selectively expressing ChR2(H134R) in AiB interneurons under the expression of a inx-1 promoter

Depositor

Shohei Mitani

Publication

Hori et al PLoS Genet. 2018 Jun 25;14(6):e1007477.

Insert

channel rhodopsin-2 with H134R mutation

Expression

Worm

Mutation

H134R

Promoter

inx-1

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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PB-NES-ZapCV2-Zinc dead

Plasmid

#203850

Purpose

Expressing zinc dead sensor NES-ZapCV2-Ala in cytoplasm of mammalian cells

Depositor

Amy Palmer

Publication

Rakshit et al Cell Rep. 2023 Jun 16;42(6):112656. doi:

Insert

ZapCV2-Ala zinc dead sensor

Tags

Nuclear Export Signal

Expression

Mammalian

Mutation

Cys and His in Zap1 is mutated with Ala

Promoter

CMV promoter

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pSWAP_Entry_H1_alpha

Plasmid

#184865

Purpose

pSwap plasmid part with H1_alpha homology arms

Depositor

Patrick Sobetzko

Publication

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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