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pBS593 promoterless GFPcre

Plasmid

#11959

Depositor

Brian Sauer

Publication

Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

Insert

GFP-cre (cre bacteriophage P1)

Use

Cre/Lox

Expression

Mammalian

Available since

Sept. 27, 2006

Availability

Academic Institutions and Nonprofits only
pLVX-opto-hCaspase-4 dCARD

Plasmid

#208785

Purpose

Inducibly expresses human Caspase 4 lacking the CARD domain, fused to light-activatable Cry2

Depositor

Petr Broz

Publication

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Cry2 mCherry Caspase4 dCARD (CASP4 Human)

Use

Lentiviral

Tags

Cry2 mCherry

Expression

Mammalian

Mutation

CARD domain is removed

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-RhoBAST-MS2

Plasmid

#196342

Purpose

Overexpression of circular RhoBAST-MS2

Depositor

Murat Sunbul

Publication

Englert et al Nat Commun. 2023 Jun 30;14(1):3879. doi:

Insert

Tornado-RhoBAST-MS2

Expression

Mammalian

Promoter

U6+27

Available since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pTubulin-mTagBFP2

Plasmid

#184931

Purpose

Tubulin labeling with blue fluorescent protein mTagBFP2

Depositor

Kiryl Piatkevich

Publication

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TUBA1B-mTagBFP2

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pYL-kasOp-FnCas12a3

Plasmid

#177875

Purpose

Expresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.

Depositor

Yunzi Luo

Publication

Zhang et al Front Bioeng Biotechnol. 2020 Jun 30;8:7

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Streptomyces codon optimized the FnCas12a mutant EP16

Use

CRISPR

Promoter

kasOp*

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG mEmerald-LAMP1

Plasmid

#168513

Purpose

Expression of rat LAMP1 fused with mEmerald to perform live tracking of lysosomes

Depositor

Franck Polleux

Publication

Courchet et al Cell. 2013 Jun 20;153(7):1510-25. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LAMP1 (Lamp1 Rat)

Tags

mEmerald

Expression

Mammalian

Promoter

CAG

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMaCTag-23

Plasmid

#120002

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with Halo

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Halo

Use

Pcr template

Tags

Halo

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pGem-nHyPer2:sHyper2

Plasmid

#84737

Purpose

Simultaneous expresion of nuclear and stromal HyPer2 in plant cells

Depositor

Philip Mullineaux

Publication

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Insert

HyPer2 targeted to nucleus

Tags

NLS-SV40

Expression

Plant

Promoter

pFMV/p35S

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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PCXLE-hUL + mTAGBFP2

Plasmid

#74944

Purpose

Dosage control and tracing of reprogramming episomes

Depositor

Ann Zovein

Publication

Schmitt et al Stem Cell Res Ther. 2017 Jun 5;8(1):132.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LMyc/Lin28 (MYCL, LIN28A Human)

Expression

Mammalian

Available since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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mOrange2-Golgi-7

Plasmid

#57959

Purpose

Localization: Gal-T - Golgi Complex, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Publication

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Golgi (B4GALT1 Human)

Tags

mOrange2

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only
pEft-3::Cerulean-Venus

Plasmid

#68048

Purpose

Ubiquitious expression of oxCerulean-oxVenus (dFP)

Depositor

Dana Miller

Publication

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pEft-3::Cerulean-Venus

Expression

Worm

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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SI9-ROBO1

Plasmid

#36144

Depositor

Gavin Wright

Publication

Crosnier et al BMC Biol. 2010 Jun 4;8:76. doi: 10.1186/

Insert

ROBO1 mouse monoclonal antibody (robo1 Zebrafish)

Expression

Mammalian

Promoter

CMV

Available since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
2XMyc-LRRK2-COR-Y1699C

Plasmid

#25070

Depositor

Mark Cookson

Publication

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Tags

2XMyc

Expression

Mammalian

Mutation

Contains only the COR domain aa535-1838 Ty…

Available since

Nov. 1, 2011

Availability

Academic Institutions and Nonprofits only
SI4-Jamc.2

Plasmid

#28216

Depositor

Gavin Wright

Publication

Crosnier et al BMC Biol. 2010 Jun 4;8:76. doi: 10.1186/

Insert

Jamc.2 mouse monoclonal antibody (jam3a Zebrafish)

Expression

Mammalian

Available since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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2XMyc-LRRK2-RCK-K1347A

Plasmid

#25066

Depositor

Mark Cookson

Publication

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Tags

2XMyc

Expression

Mammalian

Mutation

Contains only the ROC-COR-Kinase domains aa 1329…

Available since

June 29, 2010

Availability

Academic Institutions and Nonprofits only
pAc5.1B-EGFP-DmDCP2-V5His6

Plasmid

#22410

Depositor

Elisa Izaurralde

Publication

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

decapping protein 2, variant A (DCP2 Fly)

Use

Drosophila expression

Tags

EGFP and V5-His6

Available since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3

Plasmid

#21712

Depositor

Elisa Izaurralde

Publication

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

Enhancer of decapping 3 (Edc3 Fly)

Use

Drosophila expression

Tags

lambdaN-HA

Available since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmTral

Plasmid

#21721

Depositor

Elisa Izaurralde

Publication

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

Trailer hitch (tral Fly)

Use

Drosophila expression

Tags

lambdaN-HA

Expression

Mammalian

Available since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pSLPB2B-FKBP12_F36V-CasRx-tagBFP-PGK-Blasticidin

Plasmid

#187952

Purpose

FKBP12 (F36V mutant) degron-tagged CasRx fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

Depositor

Edda Schulz

Publication

Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FKBP12_F36V-CasRx-tagBFP

Use

CRISPR and Synthetic Biology

Tags

GGS linker, HA-tag, and NLS

Expression

Mammalian

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-SMYD3

Plasmid

#85650

Purpose

retroviral expression of human SMYD3

Depositor

Julien Sage

Publication

Mazur et al Nature. 2014 Jun 12;510(7504):283-7. doi

Insert

SMYD3 (SMYD3 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Promoter

LTR

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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