We narrowed to 8,239 results for: FIE

1. pLX_HA

   Plasmid

   #178534

   Purpose

   Modifies pLX_317 backbone to express c-term HA tag (instead of c-term V5 tag)

   Depositor

   William Hahn

   Article

   Pan et al Cell Syst. 2022 Jan 21. pii: S2405-4712(

   Type

   Empty backbone

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDonor_RB-TnV2

   Plasmid

   #213907

   Purpose

   Barcoded mariner transposon with kan selection casette, and modified terminal sequences - barcodes can be added via FseI-SbfI restriction sites

   Depositor

   James Collins

   Article

   English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

   Insert

   mariner transposon with kan resistance and FseI-SbfI sites for barcode insertion
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p5E-kdrl

   Plasmid

   #78687

   Purpose

   kdrl promoter for Gateway cloning

   Depositor

   Jau-Nian Chen, Sarah Childs

   Article

   Choi et al Dev Biol. 2007 Apr 15;304(2):735-44. Epu

   Insert

   kdrl promoter (kdrl Zebrafish)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   kdrl

   Available since

   June 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. JH01

   Plasmid

   #73510

   Purpose

   GFPo_PH pCFJ150 - contains C. elegans sequence optimized GFP

   Depositor

   Bob Goldstein

   Article

   Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

   Insert

   pmex-5::GFPo::PH::tbb-2
   

   Use

   Tags

   GFP

   Expression

   Mutation

   Promoter

   mex-5

   Available since

   Feb. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSW454 - pUFO

   Plasmid

   #115978

   Purpose

   entry vector for GreenGate cloning method, promoter

   Depositor

   Jan Lohmann

   Article

   Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

   Insert

   pUFO
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ152

   Plasmid

   #69303

   Purpose

   Gateway entry vector with pco-dCas9-VP64

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Insert

   pco-dCas9-VP64 (Plant codon-optimized)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   VP64 activator fusion; NLS fusion

   Promoter

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEXPqcxip-hSURF4-FLAG

   Plasmid

   #159139

   Purpose

   Hu SURF4 -flag mammalian expression Gateway vector.

   Depositor

   Martin Dorf

   Article

   Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

   Insert

   surfeit 4 (SURF4 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV (cytomegalovirus) enhancer and the MSV (mouse…

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. JC201: pMVP/Ad/5-35-DEST

   Plasmid

   #121847

   Purpose

   pMVP destination vector, empty 5/35 fiber-modified adenovirus vector backbone

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Adenoviral; Pmvp gateway destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-sh1368 knockdown c-myc

   Plasmid

   #29435

   Depositor

   Bob Eisenman

   Article

   Lin et al EMBO J. 2009 Oct 21. 28(20):3157-70.

   Insert

   c-Myc shRNA (MYC Human)
   

   Use

   Lentiviral and RNAi

   Tags

   GFP and WPRE

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pXJ-Nanoluc (Nluc)

    Plasmid

    #232656

    Purpose

    Nanoluc luciferase expression driven CMV promoter

    Depositor

    Chen-Yang Shen

    Article

    Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

    Insert

    Nanoluc
    

    Use

    Luciferase

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCS2-LAMP1-EGFP

    Plasmid

    #169003

    Purpose

    Express rat LAMP1-EGFP in zebrafish

    Depositor

    Noboru Mizushima

    Article

    Morishita et al Cell Rep. 2020 Dec 8;33(10):108477. doi:

    Insert

    rat LAMP1-EGFP
    

    Use

    Unspecified

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDD282

    Plasmid

    #66823

    Purpose

    GFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    GFP-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized GFP

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. OA984

    Plasmid

    #120363

    Purpose

    Expresses an single chain antibody 1C19 that targets all four DENV serotypes and expresses a red fluorescent protein reporter

    Depositor

    Omar Akbari

    Article

    Buchman et al PLoS Pathog. 2020 Jan 16;16(1):e1008103.

    Insert

    1C19 single chain antibody
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    AAEL010782 carboxypeptidase A (CPA) gene Promoter

    Available since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTorPE-jREX-GECO1

    Plasmid

    #113941

    Purpose

    Bacterial expression of red fluorescent Ca2+ sensor jREX-GECO1

    Depositor

    Thomas Hughes, Robert Campbell

    Article

    Molina et al Biophys J. 2019 May 21;116(10):1873-1886

    Insert

    jREX-GECO1
    

    Use

    Tags

    6xHis and Modified TorA MNNNDLFQASRRRFLAQLG[G to …

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBluescriptSKII+ U6-sgRNA(F+E) empty

    Plasmid

    #74707

    Purpose

    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and BbsI sites that allow insertion of a spacer sequence

    Depositor

    Eugene Yeo

    Article

    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

    Insert

    U6 promoter driving sgRNA with Bbsi sites for insertion of spacer sequence
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNCS mTangerine

    Plasmid

    #91763

    Purpose

    Bacterial expression of a fluorescent protein, monomeric Tangerine (mTangerine)

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

    Insert

    monomeric Tangerine
    

    Use

    Tags

    no tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLVX-3XMyc-mTrim25

    Plasmid

    #51394

    Depositor

    Narry Kim

    Article

    Kwon et al Nat Struct Mol Biol. 2013 Sep;20(9):1122

    Insert

    Trim25 (Trim25 Mouse)
    

    Use

    Lentiviral

    Tags

    3XMyc

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pYPQ153

    Plasmid

    #69304

    Purpose

    Gateway entry vector with pco-dCas9-3X(SRDX)

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    pco-dCas9-3X(SRDX) (Plant codon-optimized)
    

    Use

    CRISPR

    Tags

    2XFLAG

    Expression

    Mutation

    SRDX (X3) repressor fusion; NLS fusion

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pB-TREtight-EGFP

    Plasmid

    #182967

    Purpose

    For making cell lines for titering pRV∆L-5tTA virus

    Depositor

    Ian Wickersham

    Article

    Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    EGFP
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Cherry-ICP0FxE-C3

    Plasmid

    #134562

    Purpose

    Expresses Cherry-ICP0 fusion protein with a RING finger deletion (FXE)

    Depositor

    Susan Janicki

    Article

    Newhart et al J Cell Sci. 2012 Nov 15;125(Pt 22):5489-

    Insert

    ICP0
    

    Use

    Tags

    Cherry

    Expression

    Mammalian

    Mutation

    RING finger deletion (FXE)

    Promoter

    CMV

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. p2R3z-BASI-ccdB-BSAI

    Plasmid

    #118389

    Purpose

    Entry clone containing ccdB negative selection marker. Used to clone any DNA fragments of interest; goldengate and gibson assembly cloning compatible. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

    Insert

    ccdB
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    See Depositor Comments Section

    Promoter

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PKD1-F (pCI-PKD1-Flag)

    Plasmid

    #21369

    Depositor

    Gregory Germino

    Article

    Hanaoka et al Nature. . 408(6815):990-4.

    Insert

    autosomal dominant polycystic kidney disease type I (PKD1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    wild-type sequence*

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEn-Chimera

    Plasmid

    #61432

    Purpose

    Gateway-Entry vector containing AtU6-26 promoter and sgRNA backbone

    Depositor

    Holger Puchta

    Article

    Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

    Insert

    AtU6-26:sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU6-26

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. modRNAc0-Cas9-2A-Puro

    Plasmid

    #172855

    Purpose

    IVT template plasmid for making modified Cas9Puro RNA

    Depositor

    Xiaojun Lian

    Article

    Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

    Insert

    Cas9-2A-Puro
    

    Use

    Ivt template plasmid

    Tags

    2A-Puro

    Expression

    Mutation

    Promoter

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAtetO_6xHis-Bst-LF

    Plasmid

    #145799

    Purpose

    Plasmid for the aTc-inducible expression of the large-fragment of Bst DNAP

    Depositor

    Andrew Ellington

    Article

    Bhadra et al mSphere. 2021 May 19;6(3):e00911-20. doi

    Insert

    Bst-LF
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    tet PA

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pDEST14-Cys-SpyCatcher002

    Plasmid

    #102829

    Purpose

    Bacterial expression of Cysteine-modified SpyCatcher002 allowing reaction with thiol coupling reagents

    Depositor

    Mark Howarth

    Article

    Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

    Insert

    Cys-SpyCatcher002
    

    Use

    Tags

    GSSGS, His6, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Cysteine residue in N-terminal region

    Promoter

    T7

    Available since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDD268

    Plasmid

    #132523

    Purpose

    mNG^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Daniel Dickinson, Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    mNG-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized mNG

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. JH02

    Plasmid

    #73511

    Purpose

    mCherryo_PH pCFJ150 - contians C. elegans sequence optimized mCherry

    Depositor

    Bob Goldstein

    Article

    Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

    Insert

    pmex-5::mCherryo::PH::tbb-2
    

    Use

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    pmex-5

    Available since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pNCS mHoneydew

    Plasmid

    #91760

    Purpose

    Bacterial expression of a fluorescent protein, monomeric Honeydew (mHoneydew)

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

    Insert

    monomeric Honeydew
    

    Use

    Tags

    no tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAF259

    Plasmid

    #105494

    Purpose

    pRPF185 derived plasmid encoding an anhydrotetracyclin inducible BitlucOpt

    Depositor

    Wiep Klaas Smits

    Article

    Oliveira Paiva et al J Mol Biol. 2019 Jan 8. pii: S0022-2836(

    Insert

    Bitlucopt
    

    Use

    Atc-dependent expression in c. difficile

    Tags

    Expression

    Mutation

    Promoter

    Ptet

    Available since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLentiGuide_mCherry_sgEPAS1#1

    Plasmid

    #183122

    Purpose

    pLentiGuide modified to express mCherry and an sgRNA targeting EPAS1

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    sgEPAS1-1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAsp-AMA-gpdA-ptr

    Plasmid

    #191016

    Purpose

    CRISPR vector for cloning sgDNAs to target genes of several Aspergillus species (especially section Flavi)

    Depositor

    Perng-Kuang Chang

    Article

    Chang et al Microbiol Spectr. 2023 Feb 14;11(1):e046

    Type

    Empty backbone

    Use

    Unspecified; Aspergillus

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Plasmid L5 attB::Pleft* mCherry

    Plasmid

    #169411

    Purpose

    Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mCherry

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    pLeft
    mCherry
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Plasmid L5 attB:Psmyc double blue

    Plasmid

    #170050

    Purpose

    Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for double blue

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    mTagBFP2
    CO BFP
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAtetO_6xHis-Klentaq

    Plasmid

    #157862

    Purpose

    Plasmid for the aTc-inducible expression of the Klentaq

    Depositor

    Andrew Ellington

    Article

    Milligan et al Biochemistry. 2018 Aug 7;57(31):4607-461

    Insert

    Klentaq
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    tet PA

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCS2-Mito-mRFP

    Plasmid

    #169001

    Purpose

    Express Mito (Su9)-mRFP in zebrafish

    Depositor

    Noboru Mizushima

    Article

    Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

    Insert

    Mito (Su9)-mRFP (encoding a mitochondrial matrix targeting sequence of subunit 9 of the V0 ATPase Su9 of N. crassa)
    

    Use

    Unspecified

    Tags

    mRFP

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pFS478

    Plasmid

    #89066

    Purpose

    KanMX Z3EV promoter cassette

    Depositor

    Nick Rhind

    Article

    Ohira et al Yeast. 2017 Aug;34(8):323-334. doi: 10.1

    Insert

    Z3EV promoter cassette
    

    Use

    Yeast integration vector

    Tags

    Expression

    Mutation

    yeast Gal1 promoter modified with multiple Zif268…

    Promoter

    Z3EV promoter

    Available since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. T7-RelA(K310R)

    Plasmid

    #23250

    Depositor

    Warner Greene

    Article

    Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

    Insert

    NFkB RelA (RELA Human)
    

    Use

    Tags

    T7 gp10

    Expression

    Mammalian

    Mutation

    K310R

    Promoter

    Available since

    March 15, 2010

    Availability

    Academic Institutions and Nonprofits only

39. pCS2-NfsB.zf1

    Plasmid

    #61387

    Purpose

    mRNA synthesis of bacterial nitroreductase NfsB codon modified for zebrafish

    Depositor

    Harold Burgess

    Article

    Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

    Insert

    nfsB.zf1
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    E28G

    Promoter

    sp6

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCS2-LAMP1-mRFP

    Plasmid

    #169004

    Purpose

    Express rat LAMP1-mRFP in zebrafish

    Depositor

    Noboru Mizushima

    Article

    Morishita et al Cell Rep. 2020 Dec 8;33(10):108477. doi:

    Insert

    rat LAMP1-mRFP
    

    Use

    Unspecified

    Tags

    mRFP

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge