We narrowed to 5,897 results for: dna plasmids

1. LgBiT-CYBA:CYBB-SmBiT vector

   Plasmid

   #237059

   Purpose

   Express LgBiT-CYBA:CYBB-SmBiT Fusion Proteins in Mammalian Cells under a CMV promoter

   Depositor

   Promega Corporation

   Has Service

   DNA

   Inserts

   CYBA (CYBA Human)
   CYBB (CYBB Human)
   

   Tags

   LgBiT and SmBiT

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available Since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. NanoBRET Assay Vector, HaloTag-KRAS(G13D)

   Plasmid

   #236856

   Purpose

   Express HaloTag(R)-KRAS(G13D) in Mammalian Cells under a CMV promoter

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   KRAS (KRAS Human)
   

   Tags

   HaloTag (R)

   Expression

   Mammalian

   Mutation

   G13D

   Promoter

   CMV

   Available Since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. SmBiT-KRAS4B(G12D) BiBiT-Ready Vector

   Plasmid

   #236798

   Purpose

   Express SmBiT-KRAS4B(G12D) Fusion Protein in Mammalian Cells under a CMV promoter

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   KRAS4B(G12D) (KRAS Human)
   

   Tags

   SmBiT

   Expression

   Mammalian

   Mutation

   G12D

   Promoter

   CMV

   Available Since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. TBXTA-c021

   Plasmid

   #139758

   Purpose

   Protein expression/crystallography (PDB:5QT0)

   Depositor

   Opher Gileadi

   Insert

   TBXT, DNA-binding domain, G177D (TBXT Human)
   

   Tags

   His6-SUMO

   Expression

   Bacterial

   Mutation

   G177D; contains only amino acids E41-N211

   Promoter

   T7

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. TBXTA-c024

   Plasmid

   #139761

   Purpose

   Protein expression/GST pull-down

   Depositor

   Opher Gileadi

   Insert

   TBXT, DNA-binding domain, G177D (TBXT Human)
   

   Tags

   His6-GST-TEV

   Expression

   Bacterial

   Mutation

   G177D; contains only amino acids E41-D225

   Promoter

   T7

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. TBXTA-c023

   Plasmid

   #139760

   Purpose

   Protein expression/GST pull-down

   Depositor

   Opher Gileadi

   Insert

   TBXT, DNA-binding domain, WT (TBXT Human)
   

   Tags

   His6-GST-TEV

   Expression

   Bacterial

   Mutation

   Contains only amino acids E41-D225

   Promoter

   T7

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEM5Z(+)-EGFP

   Plasmid

   #65206

   Purpose

   EGFP expression plasmid (also called p111) for the creation of a heteroduplex DNA repair assay plasmid with p189. *Please see notes in comments section.

   Depositor

   LuZhe Sun

   Article

   Zhou et al Anal Biochem. 2009 May 1;388(1):167-9. d

   Insert

   EGFP
   

   Expression

   Bacterial and Mammalian

   Mutation

   Restriction enzyme recognition sites added either…

   Promoter

   CMV

   Available Since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. Nluc-YAP1_TEAD4-HaloTag Bidirectional(Balanced) Vector

   Plasmid

   #237003

   Purpose

   Express NLuc-YAP1_TEAD4-HaloTag(R) Fusion Proteins in Mammalian Cells under a CMV promoter

   Depositor

   Promega Corporation

   Has Service

   DNA

   Inserts

   YAP1 (YAP1 Human)
   TEAD4 (TEAD4 Human)
   

   Tags

   HaloTag (R) and NanoLuc (R)

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available Since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pDONR207 SARS-CoV-2 NSP3

   Plasmid

   #141257

   Purpose

   Gateway-compatible Entry vector, with insert of NSP3 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Has Service

   Cloning Grade DNA

   Insert

   NSP3 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   April 1, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pDONR207 SARS-CoV-2 E

    Plasmid

    #141273

    Purpose

    Gateway-compatible Entry vector, with insert of the envelope (E) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Has Service

    Cloning Grade DNA

    Insert

    E (E SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    April 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pDONR207 SARS-CoV-2 M

    Plasmid

    #141274

    Purpose

    Gateway-compatible Entry vector, with insert of the membrane (M) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Has Service

    Cloning Grade DNA

    Insert

    M (M SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    April 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pICOZ-MAG[EF1α-CD19 4-1BB-eGFP]

    Plasmid

    #218344

    Purpose

    Donor plasmid of MAG Transposon with CD19 CAR mammalian expression cassette

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    CD19 CAR mammalian expression cassette
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pICOZ-SB100X[EF1α-CD19 4-1BB-eGFP]

    Plasmid

    #218343

    Purpose

    Donor plasmid of SB100X Transposon with CD19 CAR mammalian expression cassette

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    CD19 CAR mammalian expression cassette
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-i53-DM

    Plasmid

    #92171

    Purpose

    recombinant adeno-associated viral plasmid for delivery and expression of ubiquitin variant that has reverted mutations L69P and V70L (i53-DM)

    Depositor

    Daniel Durocher

    Article

    Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d

    Insert

    i53-DM
    

    Use

    AAV

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    UbvG08 with I44A, P69L, and L70V mutations and no…

    Promoter

    CMV

    Available Since

    July 13, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pET-MBP-NLS-Geo_st

    Plasmid

    #87703

    Purpose

    Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP and SV40 NLS

    Depositor

    Jennifer Doudna

    Insert

    GeoCas9
    

    Tags

    10xHis-MBP-TEV

    Expression

    Bacterial

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDY1330 STITCHR pCMV-nCas9-XTEN-v170_R2Tocc

    Plasmid

    #234826

    Purpose

    STITCHR R2Tocc editor

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

    Insert

    nCas9h840a-xten-R2Tocc
    

    Use

    CRISPR

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSL1142 (pSPIN, pBBR1 backbone)

    Plasmid

    #160730

    Purpose

    Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pBBR1 backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins, crRNA, and donor DNA
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSPgRNA

    Plasmid

    #47108

    Purpose

    Expresses a S. pyogenes Cas9/dCas9 guide RNA in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Has Service

    Cloning Grade DNA

    Insert

    SPgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSL1521 (pSPIN, pSC101* backbone)

    Plasmid

    #160729

    Purpose

    Single-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins, crRNA, and donor DNA
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSL1765 (pSPIN-R, R*MmeI)

    Plasmid

    #160734

    Purpose

    Single-plasmid V. cholerae CAST. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA with BsaI sites. Reverse (R) order minimizes self-targeting; MmeI site for Tn-seq; pBBR1 backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins, crRNA, and donor DNA
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge