Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 18,759 results for: nls

Clear keywords
Showing: 3241 - 3260 of 18759 results

  1. pL6puro-pSV40(4)-pmRevErbA-Venus-NLS-Pest

    Plasmid
    #240115
    Purpose
    A lentiviral (yellow) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by SV40 promoter.
    Depositor
    Achim Kramer
    Publication
    Gabriel et al bioRxiv 2025.06.30.661926
    Insert
    murine Nr1d1 promoter+Venus-NLS-Pest-Reporter (Nr1d1 Mouse)
    Use
    Lentiviral
    Mutation
    added SV40-Enhancer+Ori
    Available since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    240115-plasmid-map-sequence-id-481765

    Enlarge

  2. pL6puro-pSV40(2)-pmRevErbA-Venus-NLS-Pest

    Plasmid
    #240113
    Purpose
    A lentiviral (yellow) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by SV40 promoter.
    Depositor
    Achim Kramer
    Publication
    Gabriel et al bioRxiv 2025.06.30.661926
    Insert
    murine Nr1d1 promoter+Venus-NLS-Pest-Reporter (Nr1d1 Mouse)
    Use
    Lentiviral
    Mutation
    added SV40-Enhancer+Ori
    Available since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    240113-plasmid-map-sequence-id-481756

    Enlarge

  4. pLenti_EF1α-NES-Kinprola_PKA_T/A-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40

    Plasmid
    #233372
    Purpose
    EF1α driven co-expression of the inactive PKA activity recorder negative control with T/A mutation Kinprola_PKA_T/A fused to mEGFP and Cas9 expression through lentivirus transduction
    Depositor
    Kai Johnsson
    Publication
    Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038
    Inserts
    NES-Kinprola_PKA_T/A-mEGFP
    FLAG-SV40NLS-Cas9-NLS-T2A-BSD
    Use
    Lentiviral
    Tags
    FLAG, NES, and mEGFP
    Promoter
    EF1α
    Available since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    233372-plasmid-map-sequence-id-466777

    Enlarge

  5. pAAV_hSyn_HA-TALE(HD)-Tet1S-NLS-SID4X_2A_EGFP_W3SL

    Plasmid
    #172208
    Purpose
    AAV vector expressing an HA-tagged TALE-SID4X transcriptional repressor protein targeting the mouse Tet1S promoter. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.
    Depositor
    Garrett Kaas
    Publication
    Greer et al J Neurosci. 2021 Jan 27;41(4):578-593. d
    Insert
    HA-TALE(HD)-Tet1S-NLS-SID4X_2A_EGFP_W3SL
    Use
    AAV and Mouse Targeting; Tale
    Tags
    HA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Available since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    172208-plasmid-map-sequence-id-338858

    Enlarge

  6. pAAV_hSyn_HA-TALE(NG)-Tet1FL-NLS-SID4X_2A_EGFP_W3SL

    Plasmid
    #172205
    Purpose
    AAV vector expressing an HA-tagged TALE-SID4X transcriptional repressor protein targeting the mouse Tet1FL promoter. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.
    Depositor
    Garrett Kaas
    Publication
    Greer et al J Neurosci. 2021 Jan 27;41(4):578-593. d
    Insert
    HA-TALE(NG)-Tet1FL-SID4X-NLS_2A_EGFP_W3SL
    Use
    AAV and Mouse Targeting; Tale
    Tags
    HA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Available since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    172205-plasmid-map-sequence-id-338845

    Enlarge

  10. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-gs-UGI-NLS(SV40)(enAsBE1.4) (RTW1028)

    Plasmid
    #114084
    Purpose
    CAG promoter expression plasmid for human codon optimized enAsBE1.4
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.4)
    Tags
    2x SV40 NLS and SV40 NLS
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A, human codon optimi…
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    114084-plasmid-map-sequence-id-230536

    Enlarge

  11. pEG302 22aa SunTag NtDRMcd SUP

    Plasmid
    #115489
    Purpose
    CRISPR-Cas9 SunTag system to target NtDRMcd to the SUPERMAN locus with two guide RNAs
    Depositor
    Steven Jacobsen
    Publication
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    April 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    115489-plasmid-map-sequence-id-242029

    Enlarge

  13. pLV_hPGK-2xMeCP2NLS-Cas9-HA-2xSV40NLS-NpNLS-P2A/T2A-mTagBFP2-2xMycNLS-WPRE

    Plasmid
    #254801
    Purpose
    Constituitve expression of spCas9 with optimized nuclear localization in iPSC-derived neurons via lenti delivery
    Depositor
    Ophir Shalem
    Publication
    Cajka et al bioRxiv [Preprint]. 2024 Oct 19:2024.10.
    Insert
    2xMeCP2NLS-Cas9-HA-2xSV40NLS-NpNLS-P2A/T2A-mTagBFP2-2xMycNLS
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    hPGK
    Available since
    Aug. 31, 2026
    Availability
    Academic Institutions and Nonprofits only
    254801-plasmid-map-sequence-id-520110

    Enlarge

  14. ePPEmax*

    Plasmid
    #205244
    Purpose
    For plant prime editing in wheat plants or monocotyledons protoplasts
    Depositor
    Zong Yuan
    Publication
    Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:
    Insert
    c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
    Use
    CRISPR
    Expression
    Plant
    Mutation
    Detailed in manuscript
    Available since
    Feb. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    205244-plasmid-map-sequence-id-422881

    Enlarge

  19. pL6puro-pSV40(4)-pmRevErbA-mScaI3-NLS-Pest

    Plasmid
    #240118
    Purpose
    A lentiviral (red) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by SV40 promoter.
    Depositor
    Achim Kramer
    Publication
    Gabriel et al bioRxiv 2025.06.30.661926
    Insert
    murine Nr1d1 promoter+mScarlet-I3-NLS-Pest-Reporter (Nr1d1 Mouse)
    Use
    Lentiviral
    Mutation
    added SV40-Enhancer+Ori
    Available since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    240118-plasmid-map-sequence-id-481753

    Enlarge

  20. pcDNA3-mCherry-TSC2(AA,3Q)-NLS

    Plasmid
    #192445
    Purpose
    Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity; targeted to the nucleus.
    Depositor
    Jin Zhang
    Publication
    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q )-NLS (TSC2 Human)
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …
    Promoter
    CMV
    Available since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    192445-plasmid-map-sequence-id-379137

    Enlarge

Showing: 3241 - 3260 of 18759 results