We narrowed to 2,365 results for: DAP

1. qTAG-N-Solo-mStayGold-CETN2

   Plasmid

   #227292

   Purpose

   Donor template for mStayGold insertion into the N-terminus of the CETN2 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px330-CETN2 (Addgene #227291)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   CETN2 Homology Arms flanking a mStayGold Tag (CETN2 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-C-Solo-mStayGold-CANX

   Plasmid

   #227280

   Purpose

   Donor template for mStayGold insertion into the C-terminus of the CANX locus. For ER visualization. To be co-transfected with sgRNA plasmid px330-CANX (Addgene #227279)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   CANX Homology Arms flanking a mStayGold Tag (CANX Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330-PITCh-ACTB

   Plasmid

   #207748

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of ACTB for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of ACTB (ACTB Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. qTAG-C-Solo-mStayGold-VIM

   Plasmid

   #227302

   Purpose

   Donor template for mStayGold insertion into the C-terminus of the VIM locus. For intermediate filament visualization. To be co-transfected with sgRNA plasmid px330-PITCh-VIM (Addgene #227301)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   VIM Homology Arms flanking a mStayGold Tag (VIM Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. qTAG-C-Solo-mStayGold-TOMM20

   Plasmid

   #227307

   Purpose

   Donor template for mStayGold insertion into the C-terminus of the TOMM20 locus. For mitochondria visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TOMM20 (Addgene #207789)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TOMM20 Homology Arms flanking a mStayGold Tag (TOMM20 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. qTAG-C-Solo-mStayGold-MAPRE1

   Plasmid

   #227324

   Purpose

   Donor template for mStayGold insertion into the C-terminus of the MAPRE1 locus. For growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 (Addgene #207793)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   MAPRE1 Homology Arms flanking a mStayGold Tag (MAPRE1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. qTAG-C-miRFP670nano3-Blast-H3C2

   Plasmid

   #207784

   Purpose

   Donor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H3C2 Homology Arms flanking a miRFP670nano3-Blast Cassette (H3C2 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pYX233-DHFR

   Plasmid

   #163761

   Purpose

   Gal-inducible yeast expression of cytosolic DHFR

   Depositor

   Johannes Herrmann

   Article

   Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

   Insert

   DHFR (Dhfr Mouse)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available Since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. hRAP-pEZT-BM

   Plasmid

   #177986

   Purpose

   Expression human RAP containing a C-terminal 6xHis tag in mammalian cells

   Depositor

   Lora Hooper

   Article

   Bang et al Science. 2021 Sep 17;373(6561):eabf9232.

   Insert

   LDL receptor related protein associated protein 1 (LRPAP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Str-KDEL-SBP-EGFP-LAMP1

    Plasmid

    #222317

    Purpose

    Synchronize the trafficking of LAMP1 from the ER.

    Depositor

    Franck Perez

    Article

    Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

    Insert

    Streptavidin-KDEL and LAMP1 fused to SBP-EGFP (LAMP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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