We narrowed to 8,239 results for: FIE
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Plasmid L5 attB:Psmyc double green
Plasmid#169412PurposeChromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for double greenDepositorInsertsmWasabi
mWasabi CO
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
Plasmid L5 attB:Psmyc triple red
Plasmid#169413PurposeChromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for triple redDepositorInsertsmScarlet proper
COmScarlet
mRuby3
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
PLKO-mcherry-luc-puro-miR-211
Plasmid#29784DepositorInsertmiR-211
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJune 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
RIP Cre-ER (DM#265)
Plasmid#15029DepositorInsertRat insulin promoter (RIP) (Ins2 Rat)
UseCre/Lox; For making transgenic constructsTagsCre-ER (TM)ExpressionMammalianMutationPromoterAvailable sinceOct. 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRTC2-puro
Plasmid#190567PurposeExpresses the full-length human L1 (L1.3) retrotransposon and an engineered neomycin retrotransposition reporter to monitor retrotransposition efficiency in cultured mammalian cells.DepositorInsertL1 (L1.3) with reporter cassette
UseTagsExpressionMammalianMutationPromoterSV40Available sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSW610 - GR-LhG4_BD
Plasmid#115992Purposeentry vector for GreenGate cloning method, CDS, with adaptors for B and D modulesDepositorInsertGR-LhG4_BD
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSW181 - GR-LhG4
Plasmid#115983Purposeentry vector for GreenGate cloning method, CDSDepositorInsertGR-LhG4
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceNov. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6(gCD44v2)-EF1a-BFP-Puro-Cas9(FZ)
Plasmid#117134PurposeAll-in-one expression vector for Cas9 and gRNA against CD44DepositorInsertgCD44 v2 (Cd44 Synthetic)
UseCRISPR and LentiviralTagsExpressionMutationDeleted BbsI site within WPRE, modified BFPPromoterhuman U6 promoter, human EF1a promoterAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pEThT-SAHS1
Plasmid#90025PurposeTo express His-tagged SAHS1 in bacteriaDepositorInsertSAHS1
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV3Tag8li-hTXLNA-HA
Plasmid#165472PurposeHuman TXLNA mammalian expression vector with C-terminal HA tag.DepositorInserttaxilin alpha (TXLNA Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
Codon Optimized Dysferlin
Plasmid#67878PurposeThe human dysferlin cDNA was codon optimized in an attempt to increase Dysferlin production in human cells.DepositorInsertcodon optimized Dysferlin
UseUnspecifiedTagsNoExpressionMutationPromoterNo PromoterAvailable sinceJuly 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
SHC003BSD-DelGFP
Plasmid#133301PurposeEGFP reporter was removed and the selection marker was modified as blasticidin in the construct of SHC003. This construct could be used for expression of a gene by lentiviral approach.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDD283
Plasmid#66824PurposeYPET^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertYPET-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized YPETExpressionWormMutationPromoterAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDD317 (Pmyo-2::GFP + SEC)
Plasmid#73344PurposeSEC vector carry Pmyo-2::GFP and ccdB sites for cloning homology armsDepositorInsertPmyo-2::GFP + SEC
UseCRISPR and Cre/LoxTagsGFPExpressionWormMutationPromotermyo-2Available sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBluescriptSKII+ U6-sgRNA(F+E) ACTB
Plasmid#74705PurposeEncodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of ACTB mRNADepositorInsertU6 promoter driving sgRNA targeting the 3'UTR of ACTB mRNA
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
UbG76V-eGFP-V5His_20aa
Plasmid#23970DepositorInsertUb-G76V-eGFP-V5HIS_20aa
UseTagseGFPExpressionMammalianMutationG76V mutation in Ubiquitin. Ub fused to N-termin…PromoterAvailable sinceMarch 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGEM-sgMUC1_dual-MTS-3x-Set I
Plasmid#162763PurposeSeparately expressing 3 unique sgMUC1_dual-MTS (SetI)DepositorInsertsgMUC1_dual-MTS_1; sgMUC1_dual-MTS_2; sgMUC1_dual-MTS_3
UseTagsExpressionMammalianMutationPromoterU6Available sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
Aurli_150841_GZG
Plasmid#162563PurposeInactivation of Aurli_150841 (crtIBY) in Aurantiochytrium limacinum MYA-1381 with zeocin resistance driven by native GAPDH promoter/termiator system.DepositorInsertshble
UseUnspecifiedTagsExpressionMutationPromoterGAPDHAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C3003
Plasmid#91095PurposeModule C, Promoter: CmYLCV, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9DepositorInsertGFP (+ BaeI site for GT donor cloning)
UseUnspecifiedTagsExpressionMutationPromoterCmYLCVAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSpyC-GFP
Plasmid#220191PurposesgRNA expression vector - pBG42 promoter with GFP in sgRNA siteDepositorInsertpBG42 promoter with GFP in sgRNA site
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpBG42Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSW457 - tCLV3
Plasmid#115980Purposeentry vector for GreenGate cloning method, terminatorDepositorInserttCLV3
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSW455 - pCLV3
Plasmid#115979Purposeentry vector for GreenGate cloning method, promoterDepositorInsertpCLV3
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCOLA banana sensor sfGFP
Plasmid#107367PurposeExpresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified banana DNADepositorInsertbanana-detecting toehold + sfGFP
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C3012
Plasmid#91099PurposeModule C, Promoter: PvUbi1, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9DepositorInsertGFP (+ BaeI site for GT donor cloning)
UseUnspecifiedTagsExpressionMutationPromoterPvUbi1Available sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVL26 - pSCR
Plasmid#116021Purposeentry vector for GreenGate cloning method, promoterDepositorInsertpSCR
UseUnspecifiedTagsExpressionMutationT171A by SDM to abolish Eco31I sitePromoterAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
RVD-AA5
Plasmid#173240PurposeOne-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.DepositorInsertNI-NI dimer RVD at Position5
UseUnspecifiedTagsExpressionMutationnoPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
RVD-AA9
Plasmid#173244PurposeOne-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.DepositorInsertNI-NI dimer RVD at Position9
UseUnspecifiedTagsExpressionMutationnoPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPD005 pcDNA Golden Gate
Plasmid#138749PurposeVector used to express most COMET components, modified from pcDNA3.1/HygroR(+) for BsaI/BpiI Golden Gate compatibility and to lack HygroR.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAP-AAV.FUS.3
Plasmid#222333PurposeAAV.FUS.3 Rep-Cap plasmid for production of AAV.FUS.3, a capsid with tropism for CNS neurons upon focused ultrasound mediated delivery.DepositorInsertAAV9 modified with 7-amino acid insertion between AA position 588 and 589 of VP1
UseAAVTagsExpressionMutationPromoterp41Available sinceAug. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSW614 - pREV
Plasmid#115989Purposeentry vector for GreenGate cloning method, promoterDepositorInsertpREV
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceNov. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSW618 - pAHP6
Plasmid#115991Purposeentry vector for GreenGate cloning method, promoterDepositorInsertpAHP6
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSW615 - pATHB-8
Plasmid#115990Purposeentry vector for GreenGate cloning method, promoterDepositorInsertpATHB-8
UseUnspecifiedTagsExpressionMutationA1287T to abolish Eco31I sitePromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVL15 - tSCR
Plasmid#116030Purposeentry vector for GreenGate cloning method, terminatorDepositorInserttSCR
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_hMed25
Plasmid#206958PurposeThe ORF of human Med25 cDNA amplified from the RT human liver RNA was cloned into pGEMTE vector and then subclone at XhoI/XbaI sites of the pcDNA3.2(+), the mammalian expression vector.DepositorInsertHomo sapiens mediator complex subunit 25 (MED25), transcript variant 1, mRNA (MED25 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIIS18-BsaI
Plasmid#128649PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
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Accessory plasmid 2
Plasmid#128571Purposefor cloning identified individual SPECSDepositorTypeEmpty backboneUseLentiviralTagsTagRFPExpressionMutationPromoterAvailable sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXGFPC-5 Pxyl-dcas9 (Sth3)
Plasmid#133317Purposeexpresses dcas9 (Streptococcus thermophilus #3); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xyl locus in Caulobacter crescentus; tetracycline resistanceDepositorInsertdcas9 (Streptococcus thermophilus #3)
UseUnspecifiedTagsExpressionMutationPromoterxyloseAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only
