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pPB-cT3G-cERP2-ELK1

Plasmid

#192918

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of ELK1

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

ELK1 (ELK1 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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his3MX6-ins3

Plasmid

#195040

Purpose

pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference

Depositor

Gloria Brar

Article

Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

Insert

His5+

Use

Yeast genomic targeting

Tags

S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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S1-71 pComb3xss anti-cFos nanobody

Plasmid

#145832

Purpose

Bacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-cFos (Human) recombinant llama nanobody S1-71 (FOS L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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SITS-MmCav1-EGFP-APEX2

Plasmid

#170525

Purpose

Vector expression of MmCav1-EGFP-APEX2 in Leishmania cell free system. Parton lab clone JEJ

Depositor

Rob Parton

Article

Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

Insert

Caveolin1 (Cav1 Mouse)

Use

Synthetic Biology

Tags

APEX2

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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SOX2 KO (T2A-H2B-EGFP)

Plasmid

#167969

Purpose

SOX2 CDS replacement with T2A-H2B-EGFP

Depositor

Emma Rawlins

Article

Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

Insert

SRY-Box Transcription Factor 2 (SOX2 Human)

Use

CRISPR; Donor template

Tags

H2B-EGFP

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pPRISM-Fuse-cmlc2-TagBFP

Plasmid

#117786

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

mRFP

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Pan-Nav channel [K58/35R]

Plasmid

#128623

Purpose

Mammalian Expression Plasmid of anti-Pan-Nav channel. Derived from hybridoma K58/35.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Pan-Nav Channel Pan recombinant mouse monoclonal antibody

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Cntn1-AP-His

Plasmid

#71939

Purpose

Expresses the extracellular region of the Contactin 1 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Cntn1 (Cntn1 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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GC92 pComb3xss anti-Gephyrin nanobody

Plasmid

#145826

Purpose

Bacterial expression of anti-Gephyrin (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Gephyrin (Human) recombinant llama nanobody GC92 (GPHN L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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GIV-FLAG S245A (SA)

Plasmid

#86095

Purpose

Expression GIV in mammalian cell

Depositor

Pradipta Ghosh

Article

Aznar et al Elife. 2016 Nov 4;5. pii: e20795. doi: 1

Insert

GIV/Girdin (CCDC88A Human)

Tags

3xFlag

Expression

Mammalian

Mutation

S245A

Promoter

CMV

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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Anti-MOG [L94/54]

Plasmid

#190296

Purpose

Mammalian Expression Plasmid of anti-MOG (Rat). Derived from hybridoma L94/54.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-MOG (Rattus norvegicus) recombinant Mouse monoclonal antibody (Mog Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shMBNL2-3712

Plasmid

#115461

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shMBNL2-3712 (MBNL2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shMBNL2-1082

Plasmid

#115462

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shMBNL2-1082 (MBNL2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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C2-66 pComb3xss anti-cFos nanobody

Plasmid

#145833

Purpose

Bacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-cFos (Human) recombinant llama nanobody C2-66 (FOS L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shMBNL1-3964

Plasmid

#115459

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shMBNL1-3964 (MBNL1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pQE-80L 2xHis-LRRK2 Armadillo 1-159

Plasmid

#186016

Purpose

2xHis-LRRK2 Armadillo 1-159

Depositor

Suzanne Pfeffer

Article

Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

Insert

2xHis-LRRK2 Armadillo 1-159 (LRRK2 Human)

Expression

Bacterial

Mutation

See depositor's comments below.

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pQE-80L 2xHis-LRRK2 Armadillo 350-550

Plasmid

#186018

Purpose

2xHis-LRRK2 Armadillo 350-550

Depositor

Suzanne Pfeffer

Article

Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

Insert

2xHis-LRRK2 Armadillo 350-550 (LRRK2 Human)

Expression

Bacterial

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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C1-84 pComb3xss anti-cFos nanobody

Plasmid

#145830

Purpose

Bacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-cFos (Human) recombinant llama nanobody C1-84 (FOS L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV2-CAG-mScarlet-I-Cdt1

Plasmid

#191100

Purpose

To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

Depositor

Murray Blackmore, Pantelis Tsoulfas

Article

Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

Insert

mScarlet-I-Cdt1 (30-120)

Use

AAV and Synthetic Biology

Tags

mScarlet-I fused to residues 30-120 of human Cdt1…

Expression

Mammalian

Mutation

Optimized to human codon usage

Promoter

CMV enhancer and CAG

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Co-Rest/RCOR1 [K72/8R]

Plasmid

#140066

Purpose

Mammalian Expression Plasmid of anti-Co-Rest/RCOR1 (Human). Derived from hybridoma K72/8.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Co-Rest/RCOR1 (Human) recombinant mouse monoclonal antibody (RCOR1 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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