We narrowed to 9,383 results for: bli

1. phCMV-229E-D15

   Plasmid

   #188908

   Purpose

   express truncated 229E spike for pseudovirus production

   Depositor

   David Nemazee

   Insert

   229E spike (S 229E)
   

   Expression

   Mammalian

   Mutation

   c-terminal 15aa deletion

   Promoter

   CMV

   Available Since

   Aug. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Pet28a_Human_H3.3

   Plasmid

   #42632

   Depositor

   Joe Landry

   Insert

   Human H3.3 (H3-3A Human)
   

   Tags

   Untagged

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 25, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pRSET.HaloTag.v7.CoOp.Cys-less

   Plasmid

   #195487

   Purpose

   Bacterial expression plasmid with HaloTag v7 insert; modified to replace cysteines C61, C262, and optimized to human codon bias/preference for use in oxidizing environments.

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Insert

   HaloTag.v7
   

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   C61T, C262V

   Promoter

   T7

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-GFAP-hM4D(Gi)-mCherry

   Plasmid

   #50479

   Purpose

   Gi-coupled hM4D DREADD fused with mCherry under the control of GFAP promoter

   Depositor

   Bryan Roth

   Has Service

   AAV5

   Insert

   hM4D(Gi)-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   GFAP

   Available Since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-HA-SOX4

   Plasmid

   #110367

   Purpose

   Mammalian expression of HA-tagged SOX4

   Depositor

   Carlos Moreno

   Insert

   SOX4 (SOX4 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. MUT_S1-H1

   Plasmid

   #218394

   Purpose

   3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice site

   Depositor

   Oded Regev

   Insert

   Modified CHO DHFR minigene
   

   Expression

   Mammalian

   Promoter

   tetracycline-responsive promoter

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-CAG-M-SPOTIT2

   Plasmid

   #191489

   Purpose

   Green fluorescent-based opioid sensor for the mu opioid receptor in AAV viral vector under a CAG promoter

   Depositor

   Wenjing Wang

   Insert

   M-SPOTIT2
   

   Use

   AAV

   Promoter

   CAG

   Available Since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. BirA(R118G)-HA destination vector

   Plasmid

   #53581

   Purpose

   To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID)

   Depositor

   Karl Kramer

   Type

   Empty backbone

   Tags

   BirA(R118G) and HA tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNL [NlucP/SIE/Hygro] Vector

   Plasmid

   #236833

   Purpose

   Express SIE response element in luciferase reporter assay

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   SIE
   

   Use

   Luciferase

   Tags

   NanoLuc (R)

   Mutation

   None

   Promoter

   minP

   Available Since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pENN.AAV.CamKII.GCaMP6f.WPRE.SV40

    Plasmid

    #100834

    Purpose

    AAV expression of ultrasensitive protein calcium sensor from CamKII promoter

    Depositor

    James M. Wilson

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    GCaMP6f
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

    Promoter

    CamKII

    Available Since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSpCas9(BB)-2A-miRFP670

    Plasmid

    #91854

    Purpose

    Cas9 from S. pyogenes with 2A-miRFP670, and cloning backbone for sgRNA. Modified Zhang Plasmid #62988

    Depositor

    Ralf Kuehn

    Inserts

    hSpCas9-2A-miRFP670
    BB-guide RNA
    

    Use

    CRISPR

    Tags

    2A-miRFP670 and 3XFLAG

    Expression

    Mammalian

    Promoter

    Cbh and U6

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCer-C3-Plk1-T210D

    Plasmid

    #68133

    Purpose

    Expression of Cerulean-tagged, constitutively active Plk1 in mammalian cells

    Depositor

    Catherine Lindon

    Insert

    Polo-like kinase 1 (PLK1 Human)
    

    Tags

    Cerulean

    Expression

    Mammalian

    Mutation

    T210D constitutively active version

    Promoter

    CMV

    Available Since

    Oct. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. YIp204-PADH1-AFB2

    Plasmid

    #99531

    Purpose

    S. cerevisiae expression of F-box protein AFB2 under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)

    Depositor

    Helle Ulrich

    Insert

    AFB2 (AFB2 Mustard Weed)
    

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. wt HA-dynamin 1 pcDNA3.1

    Plasmid

    #34682

    Depositor

    Sandra Schmid

    Insert

    dynamin 1ba (DNM1L Human)
    

    Tags

    HA (variant)

    Expression

    Mammalian

    Available Since

    Jan. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. PX393 Gluconacetobacter diazotrophicus Cas9

    Plasmid

    #68330

    Purpose

    Gluconacetobacter diazotrophicus Cas9

    Depositor

    Feng Zhang

    Insert

    GdCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.3_SARS2_XBB.1.5

    Plasmid

    #196585

    Purpose

    expressing SARS-CoV-2 XBB.1.5 spike protein for pseudovirus production

    Depositor

    David Nemazee

    Insert

    Spike (XBB.1.5)
    

    Expression

    Mammalian

    Mutation

    G252V, F486P on the basis of XBB

    Promoter

    CMV

    Available Since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-3'TwStrep

    Plasmid

    #208618

    Purpose

    Empty vector for mammalian expression, insert Twin Strep tag using BamHI and AflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with Twin Strep tag

    Depositor

    Quanfu Ma

    Type

    Empty backbone

    Tags

    Twin Strep

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. PX383 Lactobacillus johnsonii Cas9

    Plasmid

    #68320

    Purpose

    Lactobacillus johnsonii Cas9

    Depositor

    Feng Zhang

    Insert

    LjCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. PX386 Lactobacillus farciminis Cas9

    Plasmid

    #68323

    Purpose

    Lactobacillus farciminis Cas9

    Depositor

    Feng Zhang

    Insert

    LfCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pXR003_puroR

    Plasmid

    #219819

    Purpose

    hU6-driven sgRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #109053)

    Depositor

    Philipp Maass

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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