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We narrowed to 3,389 results for: guide rna expression plasmid

Showing: 3261 - 3280 of 3389 results

  1. pIA33

    Plasmid
    #120803
    Purpose
    E. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfp
    Depositor
    David S. Weiss, Craig Ellermeier
    Article
    Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-
    Inserts
    deactivated nuclease dCas9, codon-optimized for C. difficile
    single guide RNA targeting red fluorescent protein
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Base-pairing region targets red fluorescent prote…
    Promoter
    Pgdh and Pxyl
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120803-plasmid-map-sequence-id-235316

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  12. pFD115

    Plasmid
    #124768
    Purpose
    pFD115 carries cas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector
    Depositor
    David Bikard
    Article
    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)
    Inserts
    Ptet
    cas9
    term PpflB sgRNA (BsaI)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Ptet
    Available since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    124768-plasmid-map-sequence-id-246185

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  15. DR274

    Plasmid
    #42250
    Purpose
    guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcription
    Depositor
    Keith Joung
    Article
    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR; Zebrafish expression
    Tags
    Expression
    Worm
    Mutation
    Promoter
    T7
    Available since
    Jan. 31, 2013
    Availability
    Academic Institutions and Nonprofits only
    42250-plasmid-map-sequence-id-254852

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  16. CROPseq-Puro-24xMS2

    Plasmid
    #212629
    Purpose
    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles
    Depositor
    Paul Blainey
    Article
    Najia et al bioRxiv 2024.10.01.616098
    Insert
    EF1a-PuroR-24xMS2-WPRE-hU6-sgRNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    212629-plasmid-map-sequence-id-420222

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  17. CROPseq-Puro-2xMS2

    Plasmid
    #212627
    Purpose
    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles
    Depositor
    Paul Blainey
    Article
    Najia et al bioRxiv 2024.10.01.616098
    Insert
    EF1a-PuroR-2xMS2-WPRE-hU6-sgRNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    212627-plasmid-map-sequence-id-420278

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  18. CROPseq-Puro-12xMS2

    Plasmid
    #212628
    Purpose
    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles
    Depositor
    Paul Blainey
    Article
    Najia et al bioRxiv 2024.10.01.616098
    Insert
    EF1a-PuroR-12xMS2-WPRE-hU6-sgRNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    212628-plasmid-map-sequence-id-422312

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  19. CROPseq-Puro-4xMS2

    Plasmid
    #212626
    Purpose
    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles
    Depositor
    Paul Blainey
    Article
    Najia et al bioRxiv 2024.10.01.616098
    Insert
    EF1a-PuroR-4xMS2-WPRE-hU6-sgRNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    212626-plasmid-map-sequence-id-420274

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  20. pLC133

    Plasmid
    #198639
    Purpose
    Expression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.
    Depositor
    David Bikard
    Article
    Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.
    Insert
    dCas9
    Use
    Tags
    3x-FLAG
    Expression
    Bacterial
    Mutation
    Promoter
    pTet
    Available since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    198639-plasmid-map-sequence-id-390787

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Showing: 3261 - 3280 of 3389 results