We narrowed to 6,087 results for: nls

1. pTE3329

   Plasmid

   #107538

   Purpose

   Expresses Lb crRNA and human codon optimized LbCpf1(RR mutant) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   Lb crRNA
   hLbCpf1(RR mutant)
   

   Use

   Tags

   3xHA, NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   G532R, K595R

   Promoter

   CMV and human U6

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTE3327

   Plasmid

   #107535

   Purpose

   Expresses As crRNA and human codon optimized AsCpf1(RVR mutant) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   As crRNA
   hAsCpf1(RVR mutant)
   

   Use

   Tags

   3xHA, NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   S542R, K548V, N552R

   Promoter

   CMV and human U6

   Available since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. DNMT3ACD-CRY2-EGFP

   Plasmid

   #82556

   Purpose

   Encodes DMNT3 under LITE2.0 system

   Depositor

   Joseph Irudayaraj

   Article

   Lo et al Sci Rep. 2017 Feb 9;7:42047. doi: 10.103

   Inserts

   DNMT3A (DNMT3A Human)
   CRY2 (CRY2 Mustard Weed)
   EGFP
   

   Use

   AAV

   Tags

   2A, NLS(alpha-imp), and NLS-VP64

   Expression

   Mammalian

   Mutation

   CRY2PHR (N-terminal photolyase homology region do…

   Promoter

   EF-1alpha

   Available since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. TOPO-ZNF84

   Plasmid

   #226450

   Purpose

   For subcloning of human ZNF84 promoter or for assays using M13 phage

   Depositor

   Glen Borchert

   Article

   DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

   Insert

   ZNF84 (ZNF84 Human)
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   No

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. TOPO-CHR12:132.68MB LG4

   Plasmid

   #226454

   Purpose

   Encodes an LG4 enhancer for subcloning or for assays using M13 phage

   Depositor

   Glen Borchert

   Article

   DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

   Insert

   CHR12:132.68MB LG4
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   No

   Promoter

   Available since

   July 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. TOPO-LG4_chr5:550kb antisense

   Plasmid

   #205468

   Purpose

   Enhancer containing plasmid used for assessing enhancer::promoter interactions

   Depositor

   Glen Borchert

   Article

   DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

   Insert

   chr5:550kb LG4
   

   Use

   Synthetic Biology; Subcloning of taq-amplified pc…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. FUS LC 4QQ->AA

   Plasmid

   #226600

   Purpose

   4 glutamine pairs to alanine pairs at positions 27, 28, 92, 93, 132, 133, 157, 158

   Depositor

   Nicolas Fawzi

   Article

   Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

   Insert

   FUS LC 4QQ->AA (FUS Human)
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   4 QQ pairs replaced with AA pairs at positions 27…

   Promoter

   T7

   Available since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. FUS LC 4QQ->GG

   Plasmid

   #226601

   Purpose

   4 glutamine pairs to glycine pairs at positions 27, 28, 92, 93, 132, 133, 157, 158

   Depositor

   Nicolas Fawzi

   Article

   Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

   Insert

   FUS LC 4QQ->GG (FUS Human)
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   4 QQ pairs replaced with GG pairs at positions 27…

   Promoter

   T7

   Available since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. FUS LC 4QQ->NN

   Plasmid

   #226602

   Purpose

   4 glutamine pairs to asparagine pairs at positions 27, 28, 92, 93, 132, 133, 157, 158

   Depositor

   Nicolas Fawzi

   Article

   Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

   Insert

   FUS LC 4QQ->NN (FUS Human)
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   4 glutamine pairs to asparagine pairs at position…

   Promoter

   T7

   Available since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. TDP-43 _CTD_5A→M_IDR + 5M→A_CR

    Plasmid

    #194268

    Purpose

    Expresses 6xHis-tagged C-terminal domain of TDP-43 with TDP-43 _CTD_5A→M_IDR + 5M→A_CR

    Depositor

    Nicolas Fawzi

    Article

    Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

    Insert

    TDP-43 (TARDBP Human, Synthetic)
    

    Use

    Tags

    6XHistag

    Expression

    Bacterial

    Mutation

    A297M, A367M, A381M, A388M, A391M M322A, M323A, M…

    Promoter

    T7

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGMC00003

    Plasmid

    #166720

    Purpose

    sgRNA against mouse Kitl

    Depositor

    Raj Chari

    Article

    Rice et al Nat Commun. 2018 Nov 30;9(1):5099. doi:

    Insert

    Kitl (Kitl Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGMC00004

    Plasmid

    #166721

    Purpose

    sgRNA against mouse Kitl

    Depositor

    Raj Chari

    Article

    Rice et al Nat Commun. 2018 Nov 30;9(1):5099. doi:

    Insert

    Kitl (Kitl Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-PGK-dt/CBA-EGFP-R106bT

    Plasmid

    #163921

    Purpose

    Serves as control to pLV-PGK-dt/CBA-EGFP-106bT.

    Depositor

    John Engelhardt

    Article

    Choi et al Genes (Basel). 2020 Oct 6;11(10). pii: g

    Insert

    dtomato and EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Added 4-tandem reverse orientation-mir-106b-targe…

    Promoter

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. MBP-hnRNPA2_LC_R191K_R201K_R216K_R254K

    Plasmid

    #104467

    Purpose

    express MBP hnRNPA2 LC with 4 R to K mutations

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    R191K R201K R216K R254K

    Promoter

    Available since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. SGEP-sh-Hs-PHGDH-1956

    Plasmid

    #188670

    Purpose

    shRNA

    Depositor

    Lewis Cantley

    Insert

    shPHGDH (PHGDH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. SGEP-sh-Hs-PHGDH-1883

    Plasmid

    #188669

    Purpose

    shRNA

    Depositor

    Lewis Cantley

    Insert

    shPHGDH (PHGDH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. TC9pAB

    Plasmid

    #71310

    Purpose

    used as a template for in vitro transcription of Cas9 mRNA after linearizing with BbsI

    Depositor

    Karl Degenhardt

    Insert

    Cas9
    

    Use

    In vitro transcription

    Tags

    NLS

    Expression

    Mutation

    Promoter

    T7

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. PX385 Streptococcus pasteurianus Cas9

    Plasmid

    #68322

    Purpose

    Streptococcus pasteurianus Cas9

    Depositor

    Feng Zhang

    Insert

    SpaCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. PX381 Filifactor alocis Cas9

    Plasmid

    #68318

    Purpose

    Filifactor alocis Cas9

    Depositor

    Feng Zhang

    Insert

    FaCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. PX396 Parvibaculum lavamentivorans Cas9

    Plasmid

    #68333

    Purpose

    Parvibaculum lavamentivorans Cas9

    Depositor

    Feng Zhang

    Insert

    PlCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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