We narrowed to 5,828 results for: pcas

1. pRhaB-8xHis-ABE8e-eVRQR (LLH551)

   Plasmid

   #242658

   Purpose

   pRhaB promoter expression plasmid for ABE8e-eVRQR with an N-terminal His8-tag

   Depositor

   Benjamin Kleinstiver

   Insert

   pRhaB-8xHis-BPNLS-TadA8e-SpCas9(D10A)-eVRQR-BPNLS
   

   Use

   CRISPR

   Tags

   8x-His

   Expression

   Bacterial

   Mutation

   eVRQR mutations in SpCas9(S55R/D1135V/G1218R/R133…

   Promoter

   RhaB

   Available Since

   Nov. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX458 ACTB

   Plasmid

   #229975

   Purpose

   ecnodes for spCas9 and sgRNA targeting last intron of ActinB gene

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Insert

   ActBsgRNA and spCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. LP102: pMAGIC (R4-R3) NLS-Sp Cas9-NLS

   Plasmid

   #132927

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2xNLS SpCas9 (nuclease active) for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Insert

   humanized SpCas9
   

   Use

   CRISPR and Synthetic Biology; Pmagic gateway entr…

   Available Since

   Oct. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pScCas9

   Plasmid

   #117700

   Purpose

   Mammalian expression vector encoding WT ScCas9

   Depositor

   Joseph Jacobson

   Article

   Chatterjee et al Sci Adv. 2018 Oct 24;4(10):eaau0766. doi

   Insert

   Streptococcus canis Cas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   EF-1α

   Available Since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV_miniABEmaxNG(GG)

   Plasmid

   #187062

   Purpose

   miniABEmax(GG)-SpCas9-NG

   Depositor

   Renzhi Han

   Article

   Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

   Insert

   miniABEmax(GG)-SpCas9-NG
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFH13

   Plasmid

   #125798

   Purpose

   Level0 SpCas9, wheat codon optimized

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   SpCas9, wheat codon optimized
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFPcR5

   Plasmid

   #149484

   Purpose

   Broad-Host range expression vector, RSF1010 origin, PcaU(AM) repressor, P3B5 promoter, mRFP, Gentamicin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   PcaU(AM)-mRFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   P3B5B

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFH32

   Plasmid

   #125809

   Purpose

   Level0 SpCas9-NG, human codon optimized

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   SpCas9-NG, human codon optimized
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKPcR5

   Plasmid

   #149467

   Purpose

   Broad-Host range expression vector, RK2 and pUC origin, PcaU(AM) repressor, P3B5B promoter, mRFP, Gentamicin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   PcaU(AM)-mRFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   P3B5B

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pimEJ5GFP

    Plasmid

    #44026

    Depositor

    Jeremy Stark

    Article

    Bennardo et al PLoS Genet. 2008 Jun 27;4(6):e1000110. d

    Insert

    EJ5GFP egfp-based chromosomal break reporter
    

    Expression

    Mammalian

    Mutation

    pCAGGS promoter and eGFP separated by pgkPURO cas…

    Promoter

    pCAGGS

    Available Since

    May 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. p8266 LentiCRISPR v2 hygro sgNT-1

    Plasmid

    #193977

    Purpose

    Expression of spCas9 and non-targeting control sgRNA

    Depositor

    Elizabeth White

    Article

    Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

    Insert

    spCas9
    

    Use

    Lentiviral

    Available Since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. PX851

    Plasmid

    #62883

    Purpose

    N-term SpCas9 piece of inducible split-4 (Cas9(N)-FRB-NES split-4).

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa 2-535)
    

    Use

    CRISPR

    Tags

    FRB and NES PTK2

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    March 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. p8267 LentiCRISPR v2 hygro sgNT-2

    Plasmid

    #193978

    Purpose

    Expression of spCas9 and non-targeting control sgRNA

    Depositor

    Elizabeth White

    Article

    Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

    Insert

    spCas9
    

    Use

    Lentiviral

    Available Since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. PX853

    Plasmid

    #62885

    Purpose

    N-term SpCas9 piece of inducible split-5 (Cas9(N)-FRB-NES split-5).

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa 2-573)
    

    Use

    CRISPR

    Tags

    FRB and NES PTK2

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    March 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFH54

    Plasmid

    #128183

    Purpose

    Level1 AtUBI10p::SpCas9 (Arabidopsis codon optimized):pea3At

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    AtUBI10p::SpCas9 (Arabidopsis codon optimized):pea3At
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFH55

    Plasmid

    #125814

    Purpose

    Level0 nSpCas9-PmCDA, Arabidopsis codon optimized

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    nSpCas9-PmCDA, Arabidopsis codon optimized
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSL1045

    Plasmid

    #129525

    Purpose

    pDEF-SpCas9, Empty Vector for CRISPRi, PbEF1a promoter, 1st Generation

    Depositor

    Scott Lindner

    Article

    Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

    Insert

    dSpCas9 (Other)
    

    Use

    Plasmodium, rodent-infectious species

    Available Since

    Sept. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. CACNA1A_Halo_N_allele

    Plasmid

    #178152

    Purpose

    Donor vector for endogenous tagging of human CACNA1A at the N-terminus with halotag

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Insert

    Halotag (CACNA1A Human)
    

    Use

    Donor vector

    Available Since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFH24

    Plasmid

    #131763

    Purpose

    Level0 SpCas9, wheat codon optimized (version 2)

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    SpCas9, wheat codon optimized, version 2
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFH23

    Plasmid

    #128180

    Purpose

    Level1 ZmUBIp::SpCas9 (wheat codon optimized):NOSt

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    ZmUBIp::SpCas9 (wheat codon optimized):NOSt
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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