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Showing: 3261 - 3280 of 13590 results

  1. pCAGGS-ROSAr

    Plasmid
    #54324
    Purpose
    TALEN expression construct for targeting the murine ROSA26 locus. It recognize the sequence: GGGAGTCTTCTGGGC. Can be used with the pBS SK mCherryROSAbsr plasmid
    Depositor
    Silvestro Conticello
    Insert
    TALEN for the murine ROSA26 locus
    Use
    Mouse Targeting and TALEN
    Expression
    Mammalian
    Promoter
    CAGG
    Available Since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    54324-plasmid-map-sequence-id-124429

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  2. pLKO.3G_X7

    Plasmid
    #171213
    Purpose
    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses eGFP for selection. Please see cloning information for suggested restriction sites
    Depositor
    Jenny Wang
    Insert
    N/A; empty backbone
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Available Since
    July 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    171213-plasmid-map-sequence-id-338282

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  3. pSRKBB-empty

    Plasmid
    #59449
    Purpose
    Contains BioBrick prefixed and suffixed insert with a modified lac promoter, Shine-Delgarno sequence, 5’ & 3’ MCS, 3’ 6xHis tag.
    Depositor
    Claudia Schmidt-dannert
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    modified lac
    Available Since
    Sept. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    59449-plasmid-map-sequence-id-92571

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  4. pGAPZa-STE2-1D4

    Plasmid
    #133422
    Purpose
    Mutant form of yeast STE2 coding sequence in vector for protein expression in the methanogenic yeast Pichia pastoris, with GAP promoter.
    Depositor
    Gerhard Wagner
    Insert
    STE2 (STE2 Budding Yeast)
    Expression
    Yeast
    Mutation
    C59S, C252A
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    133422-plasmid-map-sequence-id-267645

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  5. pBS-3xpA

    Plasmid
    #176505
    Purpose
    3x SV40 polyA sequences
    Depositor
    Mario Capecchi
    Insert
    3x SV40 polyA
    Use
    Synthetic Biology
    Expression
    Mammalian
    Available Since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    176505-plasmid-map-sequence-id-346556

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  6. ORFtag_LambdaN-SD_Frame3

    Plasmid
    #220986
    Purpose
    ORFtag viral retroviral vector containing a constitutively active promoter and a LambdaN tag followed by a splice donor sequence in Frame3
    Depositor
    Alexander Stark
    Insert
    lambdaN
    Use
    Retroviral
    Expression
    Mammalian
    Available Since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    220986-plasmid-map-sequence-id-437120

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  7. ORFtag_LambdaN-SD_Frame1

    Plasmid
    #220984
    Purpose
    ORFtag viral retroviral vector containing a constitutively active promoter and a LambdaN tag followed by a splice donor sequence in Frame1
    Depositor
    Alexander Stark
    Insert
    lambdaN
    Use
    Retroviral
    Expression
    Mammalian
    Available Since
    June 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    220984-plasmid-map-sequence-id-437511

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  8. ORFtag_LambdaN-SD_Frame2

    Plasmid
    #220985
    Purpose
    ORFtag viral retroviral vector containing a constitutively active promoter and a LambdaN tag followed by a splice donor sequence in Frame2
    Depositor
    Alexander Stark
    Insert
    lambdaN
    Use
    Retroviral
    Expression
    Mammalian
    Available Since
    June 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    220985-plasmid-map-sequence-id-437496

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  9. pCAGGS-ROSAf

    Plasmid
    #54323
    Purpose
    TALEN expression construct for targeting the murine ROSA26 locus. It recognize the sequence: CTGCAACTCCAGTCT. Can be used with the pBS SK mCherryROSAbsr plasmid
    Depositor
    Silvestro Conticello
    Insert
    TALEN for the murine ROSA26 locus
    Use
    Mouse Targeting and TALEN
    Expression
    Mammalian
    Promoter
    CAGG
    Available Since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    54323-plasmid-map-sequence-id-124428

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  10. pSiM1

    Plasmid
    #199380
    Purpose
    Encodes dual cistronic membrane localized GCaMP6s and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    GCaMP6s::ras-2CAAX::SL2::mScarlet-I::ras-2CAAX
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Available Since
    April 24, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199380-plasmid-map-sequence-id-391037

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  11. pSiM4

    Plasmid
    #199383
    Purpose
    Encodes dual cistronic membrane localized jGCaMP7f and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    jGCaMP6f::ras-2CAAX::SL2::mScarlet-I::ras-2CAAX
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Available Since
    April 24, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199383-plasmid-map-sequence-id-391021

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  12. pcDNA3.1-MS2-HA-HsNeuroguidin_AA

    Plasmid
    #148237
    Purpose
    Mammalian Expression of HsNeuroguidin
    Depositor
    Elisa Izaurralde
    Insert
    HsNeuroguidin (NGDN Human)
    Expression
    Mammalian
    Mutation
    two silent mutations compared to the sequence giv…
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148237-plasmid-map-sequence-id-429663

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  13. pSiM3

    Plasmid
    #199382
    Purpose
    Encodes dual cistronic membrane localized jGCaMP7s and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    jGCaMP7s::ras-2CAAX::SL2::mScarlet-I::ras-2CAAX
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Available Since
    April 24, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199382-plasmid-map-sequence-id-391043

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  14. C35m

    Plasmid
    #120969
    Purpose
    MoClo golden gate assembly CD part for RhlR (Rhl quorum-sensing receptor for C4-HSL, synonymously modified to remove internal BbsI and BsaI cut sites).
    Depositor
    Richard Murray
    Insert
    RhlR
    Use
    Synthetic Biology
    Mutation
    BsaI and BbsI sites removed; amino acid sequence …
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120969-plasmid-map-sequence-id-238056

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  15. pBS-2xpA

    Plasmid
    #176504
    Purpose
    2x SV40 polyA sequences
    Depositor
    Mario Capecchi
    Insert
    2x SV40 polyA
    Use
    Synthetic Biology
    Expression
    Mammalian
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176504-plasmid-map-sequence-id-348023

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  16. pBS-4xpA

    Plasmid
    #176506
    Purpose
    4x SV40 polyA sequences
    Depositor
    Mario Capecchi
    Insert
    4x SV40 polyA
    Use
    Synthetic Biology
    Expression
    Mammalian
    Available Since
    Nov. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    176506-plasmid-map-sequence-id-347136

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  17. UC20m

    Plasmid
    #121040
    Purpose
    MoClo golden gate assembly DE part for gN2 gRNA (guide RNA for S. pyogenes Cas9; sequence designed with NUPACK for stable tracrRNA structure and open targeting region).
    Depositor
    Richard Murray
    Insert
    gN2 Cas9 gRNA UC part
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121040-plasmid-map-sequence-id-236353

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  18. pSpy

    Plasmid
    #29588
    Depositor
    Marc Fiedler
    Insert
    Spy (spy E.coli BL21(DE3))
    Expression
    Bacterial
    Mutation
    processed form of Spy starting at residue 24 (Rem…
    Available Since
    April 28, 2011
    Availability
    Academic Institutions and Nonprofits only

  19. mEmerald-Paxillin-KM-N-18

    Plasmid
    #54218
    Purpose
    Localization: Focal Adhesions, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Paxillin-KM
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Mutation to remove Kozak sequence
    Promoter
    CMV
    Available Since
    July 10, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. pTUB:FLuc

    Plasmid
    #114578
    Purpose
    Constitutive expression of firefly luciferase in T. gondii using the alpha tubulin promoter; floxed pyrimethamine selectable marker; UPRT locus flanking sequence to direct integration
    Depositor
    David Sibley
    Insert
    Firefly Luciferase
    Use
    Cre/Lox and Luciferase
    Promoter
    alpha tubulin T. gondii
    Available Since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    114578-plasmid-map-sequence-id-220131

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Showing: 3261 - 3280 of 13590 results