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TFORF0786

Plasmid

#142217

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

FOXN1 (FOXN1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-Ago2-FDM5

Plasmid

#72212

Purpose

Mammalian expression of aa 518-860 of human Ago2

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

argonaute RISC catalytic component 2 (AGO2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Δ 1-517

Promoter

CMV

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only
Anti-PICK1 [L20/26R]

Plasmid

#222149

Purpose

Mammalian Expression Plasmid of anti-PICK1 (Human). Derived from hybridoma L20/26.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-PICK1 (Homo sapiens) recombinant mouse monoclonal antibody. (PICK1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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Anti-Co-Rest/RCOR1 [K72/2R]

Plasmid

#222145

Purpose

Mammalian Expression Plasmid of anti-Co-Rest/RCOR1 (Human). Derived from hybridoma K72/2.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Co-Rest/RCOR1 (Homo sapiens) recombinant mouse monoclonal antibody. (RCOR1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Gamma-protocadherin-A3 [N144/5R]

Plasmid

#222154

Purpose

Mammalian Expression Plasmid of anti-Gamma-protocadherin-A3 (Mouse). Derived from hybridoma N144/5.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Gamma-protocadherin-A3 (Mus musculus) recombinant mouse monoclonal antibody. (Pcdhga3 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Gamma-protocadherin-B2 [N148/14R]

Plasmid

#222155

Purpose

Mammalian Expression Plasmid of anti-Gamma-protocadherin-B2 (Mouse). Derived from hybridoma N148/14.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Gamma-protocadherin-B2 (Mus musculus) recombinant mouse monoclonal antibody. (Pcdhgb2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Shank1 [N22/18R]

Plasmid

#222158

Purpose

Mammalian Expression Plasmid of anti-Shank1 (Rat). Derived from hybridoma N22/18.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Shank1 (Rattus norvegicus) recombinant mouse monoclonal antibody. (Shank1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Shank2 [N23B/9R]

Plasmid

#222159

Purpose

Mammalian Expression Plasmid of anti-Shank2 (Rat). Derived from hybridoma N23B/9.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Shank2 (Rattus norvegicus) recombinant mouse monoclonal antibody. (Shank2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-VAPA [N479/24R]

Plasmid

#222165

Purpose

Mammalian Expression Plasmid of anti-VAPA (Rat). Derived from hybridoma N479/24.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-VAPA (Rattus norvegicus) recombinant mouse monoclonal antibody. (Vapa Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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M22: pHR-SFFVp-NLS-iLID::EGFP::FTH1

Plasmid

#122147

Purpose

Self-assebled 24-mer tagged with NLS, iLID, and EGFP. Upon blue-light activation can bind up to 24 SspB-modified proteins.

Depositor

Clifford Brangwynne

Article

Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

Insert

FTH1 (FTH1 Human)

Use

Lentiviral

Tags

NLS-iLID-GFP

Expression

Mammalian

Promoter

SFFV

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-ARID1A-deltaIDR1-FLAG

Plasmid

#242210

Purpose

Expression of human ARID1A ∆IDR1 (deletion of a.a. 2–970) using piggyBac transposition

Depositor

Courtney Hodges

Article

Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

Insert

ARID1A (ARID1A Human)

Use

Piggybac

Tags

FLAG

Expression

Mammalian

Mutation

Deleted IDR1 from ARID1A (deletion of a.a. 2–970)

Promoter

CAG

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR223_FYN_WT

Plasmid

#82211

Purpose

Gateway Donor vector containing FYN , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FYN (FYN Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_LYN_WT

Plasmid

#82215

Purpose

Gateway Donor vector containing LYN ,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

LYN (LYN Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_IRS1_WT

Plasmid

#82214

Purpose

Gateway Donor vector containing IRS1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IRS1 (IRS1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_FH_WT

Plasmid

#82210

Purpose

Gateway Donor vector containing FH , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FH (FH Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SMAD5_WT

Plasmid

#82217

Purpose

Gateway Donor vector containing SMAD5 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SMAD5 (SMAD5 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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CXCR4-DuET

Plasmid

#213221

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

CXCR4 (CXCR4 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCjeCas9

Plasmid

#172215

Purpose

Expresses Campylobacter jejuni Cas9 (CjeCas9) in bacterial cells

Depositor

John van der Oost

Article

Saha et al Sci Adv. 2020 Jun 17;6(25):eaaz4849. doi

Insert

Cas9 endonuclease from the Campylobacter jejuni Type II CRISPR/Cas system

Tags

Histag

Promoter

T7

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_HDAC1_WT

Plasmid

#82212

Purpose

Gateway Donor vector containing HDAC1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HDAC1 (HDAC1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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TFORF0691

Plasmid

#142214

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

HNF4A (HNF4A Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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