We narrowed to 10,632 results for: t7

1. His6_BcLOV4_C292A_mCherry_BamUK

   Plasmid

   #119761

   Purpose

   Encodes mCherry-tagged BcLOV4 "dark" mutant with C292A mutation for expression in bacterial cells

   Depositor

   Brian Chow

   Article

   Glantz et al Methods Enzymol. 2019;622:249-270. doi:

   Insert

   BcLOV4-C292A_mCherry
   

   Tags

   mCherry

   Expression

   Bacterial

   Mutation

   Mutated cysteine at position 292 to alanine

   Promoter

   T7

   Available Since

   June 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pST50Trc2-DHFRHIS

   Plasmid

   #63967

   Purpose

   Position 2 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET21b-RL027A

   Plasmid

   #42133

   Depositor

   Sheref Mansy

   Article

   Lentini et al ACS Synth Biol. 2013 Mar 15.

   Insert

   mCherry-mVenus
   

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Feb. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pST50Trc4-HISDHFR

   Plasmid

   #63989

   Purpose

   Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. SaMscL (CDelta26)

   Plasmid

   #79027

   Purpose

   Staphylococcus aureus MscL with C Terminal Truncation of 26 amino acids

   Depositor

   Douglas Rees

   Article

   Liu et al Nature. 2009 Sep 3;461(7260):120-4. doi:

   Insert

   MscL
   

   Tags

   6x Histidine Tag

   Mutation

   Stop codon placed before 26 amino acids at C Term…

   Promoter

   T7 Promoter

   Available Since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET29b-pld

   Plasmid

   #128795

   Purpose

   expresses E. coli PLD in bacteria

   Depositor

   Emily Balskus

   Article

   Chittim et al Nat Microbiol. 2019 Jan;4(1):155-163. do

   Insert

   Phospholipase D
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TAL2350

   Plasmid

   #36769

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   XPC-TALEN_Left (Xpc Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pST50Trc2-HISDHFR

   Plasmid

   #63953

   Purpose

   Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pST50Trc2-FLAGNDHFR

   Plasmid

   #63961

   Purpose

   Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Tags

   FLAG; TEV cleavable

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pST50Trc3-CBPHISNDHFR

    Plasmid

    #63981

    Purpose

    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Tags

    6xHis & Calmodulin Binding Peptide (CBP); TEV…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSARSF534-3

    Plasmid

    #45618

    Depositor

    Hideo Iwai

    Article

    Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

    Insert

    NpuDnaEC35-GFP_C
    

    Tags

    H6

    Expression

    Bacterial

    Mutation

    SYG->CYG in chromophore

    Promoter

    T7

    Available Since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Human Mevalonate Kinase (MK)

    Plasmid

    #52429

    Purpose

    bacterial expression of human Mevalonate Kinase (MK)

    Depositor

    Henry Miziorko

    Article

    Potter et al J Biol Chem. 1997 Oct 10;272(41):25449-5

    Insert

    Human Mevalonate Kinase (MVK Human)
    

    Expression

    Bacterial

    Mutation

    see comments

    Promoter

    T7

    Available Since

    April 10, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pUG-301

    Plasmid

    #104730

    Purpose

    Level 2 construct

    Depositor

    Marco Trujillo

    Article

    Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

    Insert

    HIS-Ubq_UBA1_HA-UBC10_EL3
    

    Tags

    HIS-Ubq, HA-E2

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJKW0825

    Plasmid

    #119022

    Purpose

    E.coli expression plasmid for Pm4CL1

    Depositor

    Jing-Ke Weng

    Article

    Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

    Insert

    Pm4CL1
    

    Tags

    8xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pST50Trc1-DHFRFLAG

    Plasmid

    #63951

    Purpose

    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Tags

    FLAG

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAc-Cdc3-Cdc10

    Plasmid

    #131160

    Purpose

    Expression of Septin rods

    Depositor

    Nils Johnsson

    Article

    Renz et al BMC Biotechnol. 2013 Jul 26;13:60. doi:

    Insert

    Cdc3 + Cdc10
    

    Use

    Bicistronic

    Tags

    Cdc10: S Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. BtuCD

    Plasmid

    #92208

    Purpose

    Expression of BtuCD in E. coli

    Depositor

    Douglas Rees

    Article

    Locher et al Science. 2002 May 10;296(5570):1091-8.

    Inserts

    btuC (btuC )
    btuD (btuD )
    

    Tags

    10x Histidine Tag and Enterokinase Cleavage Site

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Aug. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 6xHis-MBP-huPcCas12a

    Plasmid

    #174681

    Purpose

    Expresses 6xHis-MBP tagged PcCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huPcCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 6xHis-MBP-huCMtCas12a

    Plasmid

    #174680

    Purpose

    Expresses 6xHis-MBP tagged CMtCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huCMtCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET21a-MtrR

    Plasmid

    #51188

    Depositor

    Christopher Voigt

    Article

    Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

    Insert

    MtrR
    

    Use

    Synthetic Biology

    Tags

    6x-his

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge