We narrowed to 2,365 results for: DAP

1. FcD265A-FLAG

   Plasmid

   #65420

   Purpose

   Mammalian expression plasmid for murine Fc-FLAG, used for monomeric Fc-IL2 production

   Depositor

   Dane Wittrup

   Article

   Zhu et al Cancer Cell. 2015 Apr 13;27(4):489-501.

   Insert

   FcD265A-FLAG
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D265A

   Promoter

   Modified CMV Promoter

   Available Since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

2. qTAG-N-Puro-sTagRFP-TUBA1B

   Plasmid

   #207769

   Purpose

   Donor template for Puro-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TUBA1B Homology Arms flanking a Puro-sTagRFP Cassette (TUBA1B Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. MSCV-NSD3-short

   Plasmid

   #72552

   Purpose

   expresses 3*FLAG tagged human NSD3-short

   Depositor

   Christopher Vakoc

   Article

   Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

   Insert

   NSD3-short (NSD3 Human)
   

   Use

   Retroviral

   Tags

   3*FLAG

   Expression

   Mammalian

   Mutation

   none

   Promoter

   LTR

   Available Since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTIGER_mNeonGreen::3xFLAG::dCrk

   Plasmid

   #131137

   Purpose

   UASp construct for over-expression of Drosophila Crk with N-terminal monomeric NeonGreen and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Insert

   Crk (Crk Fly)
   

   Use

   Tags

   3XFLAG and mNeonGreen

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. qTAG-C-moxGFP-Puro-H3C2

   Plasmid

   #207783

   Purpose

   Donor template for moxGFP-2A-Puro insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H3C2 Homology Arms flanking a moxGFP-Puro Cassette (H3C2 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pTIGER_tdTomato::3xFLAG::dCrk

   Plasmid

   #131138

   Purpose

   UASp construct for over-expression of Drosophila Crk with N-terminal tandem Tomato and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Insert

   Crk (Crk Fly)
   

   Use

   Tags

   3XFLAG and tdTomato

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. qTAG-N-Blast-sTagRFP-TUBA1B

   Plasmid

   #207768

   Purpose

   Donor template for Blast-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TUBA1B Homology Arms flanking a Blast-sTagRFP Cassette (TUBA1B Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. GFP-APPL1-R146A/K152A/R154A

   Plasmid

   #59767

   Purpose

   Expresses APPL1 Endosomal Localization Mutant

   Depositor

   Donna Webb

   Article

   Broussard et al Mol Biol Cell. 2012 Apr;23(8):1486-99. d

   Insert

   APPL1 (APPL1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   R146A/K152A/R154A

   Promoter

   CMV

   Available Since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pMKO.1-shCcnb1

   Plasmid

   #162793

   Purpose

   Ccnb1 shRNA in pMKO.1 retroviral vector

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Ccnb1 shRNA (Ccnb1 Mouse)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDF-casBCDE(g8)

    Plasmid

    #89727

    Purpose

    Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing 33-nt spacer (referred to as 'wt') matching a protospacer in the M13 phage genome.

    Depositor

    Konstantin Kuznedelov, Konstantin Severinov

    Article

    Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

    Insert

    CRISPR array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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