Addgene

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ECPK1D

Plasmid

#130830

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKG1

Use

Gateway entry vector

Tags

eGFP-FLAG-HA

Expression

Mutation

Promoter

Available since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pHWBF07exp

Plasmid

#130832

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKP2

Use

Tags

eGFP-FLAG-HA

Expression

Plant

Mutation

Promoter

CaMV 35S

Available since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-CAG-mRhubarb713-P2A-GFP

Plasmid

#197172

Purpose

Expresses the protein of mRhubarb713-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

mRhubard713-P2A-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-BDFP1.8-P2A-GFP

Plasmid

#197171

Purpose

Expresses the protein of BDFP1.8-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

BDFP1.8-P2A-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miRFP2-P2A-GFP

Plasmid

#197155

Purpose

Expresses the protein of miRFP2-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP2-P2A-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pPT-TAR C1

Plasmid

#141246

Purpose

Transformation Associated Recombination (TAR) cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 1

Depositor

Bogumil Karas

Article

Cochrane et al Algal Research, 2020. 50, 102014

Insert

Mitochondrial Genome of Phaeodactylum tricornutum

Use

Synthetic Biology; Diatom expression

Tags

Expression

Bacterial and Yeast

Mutation

Minor additional mutations described in manuscript

Promoter

Available since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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pmEGFP-tHe-Tub5

Plasmid

#201738

Purpose

mEGFP-tagged tardigrade (Hypsibius exemplaris) epsilon-tubulin Tub5 for expression in mammalian cells

Depositor

Stanislav Vinopal

Article

Novotna Floriancicova et al Sci Rep. 2023 Mar 30;13(1):5194. doi: 10

Insert

tHe-Tub5

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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ptHe-Tub3-mScarlet

Plasmid

#201735

Purpose

Tardigrade (Hypsibius exemplaris) gamma-tubulin Tub3 tagged with mScarlet for expression in mammalian cells

Depositor

Stanislav Vinopal

Article

Novotna Floriancicova et al Sci Rep. 2023 Mar 30;13(1):5194. doi: 10

Insert

Tubulin gamma

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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ptHe-Tub2D4-mScarlet

Plasmid

#201734

Purpose

Tardigrade (Hypsibius exemplaris) beta-tubulin Tub2D4 tagged with mScarlet for expression in mammalian cells

Depositor

Stanislav Vinopal

Article

Novotna Floriancicova et al Sci Rep. 2023 Mar 30;13(1):5194. doi: 10

Insert

Tubulin Beta D4

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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ptHe-Tub2D3-mScarlet

Plasmid

#201733

Purpose

Tardigrade (Hypsibius exemplaris) beta-tubulin Tub2D3 tagged with mScarlet for expression in mammalian cells

Depositor

Stanislav Vinopal

Article

Novotna Floriancicova et al Sci Rep. 2023 Mar 30;13(1):5194. doi: 10

Insert

Tubulin Beta D3

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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ptHe-Tub2D2-mScarlet

Plasmid

#201732

Purpose

Tardigrade (Hypsibius exemplaris) beta-tubulin Tub2D2 tagged with mScarlet for expression in mammalian cells

Depositor

Stanislav Vinopal

Article

Novotna Floriancicova et al Sci Rep. 2023 Mar 30;13(1):5194. doi: 10

Insert

Tubulin Beta D2

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
ptHe-Tub2C-mScarlet

Plasmid

#201731

Purpose

Tardigrade (Hypsibius exemplaris) beta-tubulin Tub2C tagged with mScarlet for expression in mammalian cells

Depositor

Stanislav Vinopal

Article

Novotna Floriancicova et al Sci Rep. 2023 Mar 30;13(1):5194. doi: 10

Insert

Tubulin Beta C

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pmEGFP-tHe-Tub1C1

Plasmid

#201730

Purpose

mEGFP-tagged tardigrade (Hypsibius exemplaris) alpha-tubulin Tub1C1 for expression in mammalian cells

Depositor

Stanislav Vinopal

Article

Novotna Floriancicova et al Sci Rep. 2023 Mar 30;13(1):5194. doi: 10

Insert

tHe-Tub1C1

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pmEGFP-tHe-Tub1A2

Plasmid

#201729

Purpose

mEGFP-tagged tardigrade (Hypsibius exemplaris) alpha-tubulin Tub1A2 for expression in mammalian cells

Depositor

Stanislav Vinopal

Article

Novotna Floriancicova et al Sci Rep. 2023 Mar 30;13(1):5194. doi: 10

Insert

tHe-Tub1A2

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pmEGFP-tHe-Tub1A1

Plasmid

#201728

Purpose

mEGFP-tagged tardigrade (Hypsibius exemplaris) alpha-tubulin Tub1A1 for expression in mammalian cells

Depositor

Stanislav Vinopal

Article

Novotna Floriancicova et al Sci Rep. 2023 Mar 30;13(1):5194. doi: 10

Insert

tHe-Tub1A1

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP24_T24_pRham-ilvA-relE

Plasmid

#201531

Purpose

Expressing synthetic overlapping gene, ilvA-relE. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

Depositor

Dan Park

Article

Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d

Insert

ilvA-relE

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

PrhaBAD (rhamnose)

Available since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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pJUMP24_T24_pRham-ilvA-relE_RBS3

Plasmid

#201532

Purpose

Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

Depositor

Dan Park

Article

Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d

Insert

ilvA-relE_RBS3

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

PrhaBAD (rhamnose)

Available since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBad-HaloGFP-145-146

Plasmid

#198600

Purpose

Green fluorescent Ca2+ ion indicator for bacterial expression.

Depositor

Robert Campbell

Article

Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

Insert

HaloGFP-145-146

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBad-HaloGFP-169-170

Plasmid

#198612

Purpose

Green fluorescent Ca2+ ion indicator for bacterial expression.

Depositor

Robert Campbell

Article

Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

Insert

HaloGFP-169-170

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPT-PCR C1

Plasmid

#141248

Purpose

PCR cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 1

Depositor

Bogumil Karas

Article

Cochrane et al Algal Research, 2020. 50, 102014

Insert

Reduced Mitochondrial Genome of Phaeodactylum tricornutum

Use

Synthetic Biology; Diatom expression

Tags

Expression

Bacterial and Yeast

Mutation

Repeat region removed, additional minor mutations…

Promoter

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge