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Showing: 6601 - 6640 of 8371 results

  1. MS2_SC2_S_Theta

    Plasmid
    #175951
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Theta SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Paul Freemont
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Theta (Amino Acid 319-869)
    Promoter
    T7
    Available since
    Oct. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    175951-plasmid-map-sequence-id-343941

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  2. MS2_SC2_S_Zeta

    Plasmid
    #175952
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Zeta SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Paul Freemont
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Zeta (Amino Acid 319-869)
    Promoter
    T7
    Available since
    Oct. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    175952-plasmid-map-sequence-id-343947

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  3. pChd4EosHaloTAF_Puro

    Plasmid
    #172099
    Purpose
    Targeting construct to make Chd4-Eos-Halo-Tev-Avi-3xFlag fusion
    Depositor
    Brian Hendrich
    Insert
    chromodomain helicase DNA binding protein 4 (Chd4 Mouse, Synthetic)
    Use
    Mouse Targeting
    Tags
    3xFLAG, Avi, Eos, Halo, and TEV
    Expression
    Mutation
    Promoter
    Available since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    172099-plasmid-map-sequence-id-338425

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  4. pCMV-T7-SpCas9-VRER-P2A-mScarlet (MNW003)

    Plasmid
    #174138
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    VRER=D1135V/G1218R/R1335E/T1337R
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174138-plasmid-map-sequence-id-341915

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  5. pCMV-T7-SpCas9-VRQR-P2A-mScarlet (MNW004)

    Plasmid
    #174139
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    VRQR=D1135V/G1218R/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174139-plasmid-map-sequence-id-341913

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  7. pB6-Id1-T2A-tdTomato-FNF-TK

    Plasmid
    #139281
    Purpose
    B6J Id1-T2A-tdTomato allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    Id1 (Id1 Mouse)
    Use
    Mouse Targeting
    Tags
    C-terminal fusion of T2A-tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    139281-plasmid-map-sequence-id-317908

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  8. pB6-Gfap-T2A-tdTomato-FNF-DTA

    Plasmid
    #140271
    Purpose
    B6J Gfap-T2A-tdTomato allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    Gfap (Gfap Mouse)
    Use
    Mouse Targeting
    Tags
    C-terminal fusion of T2A-tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    140271-plasmid-map-sequence-id-317910

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  9. pB6-Gfap-T2A-td-sfGFP-FNF-DTA

    Plasmid
    #137044
    Purpose
    B6J Gfap-T2A-td-sfGFP allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    Gfap (Gfap Mouse)
    Use
    Mouse Targeting
    Tags
    T2A-td-sfGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    137044-plasmid-map-sequence-id-274424

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  13. Ad-mMKP3 S159A S197A-Flag

    Plasmid
    #131579
    Purpose
    Expresses MKP-3 with S159A mutation and S197A mutation in mammalian cells
    Depositor
    Haiyan Xu
    Insert
    Mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
    Use
    Adenoviral
    Tags
    Flag
    Expression
    Mutation
    changed Serine 159 to Alanine and Serine 197 to A…
    Promoter
    CMV
    Available since
    Oct. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    131579-plasmid-map-sequence-id-256812

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  14. Mouse 3' Ubf AID GFP PuroR

    Plasmid
    #127901
    Purpose
    HDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Mouse Ubf Gene
    Depositor
    Mark Groudine
    Insert
    Ubf (Ubtf Mustard Weed, Mouse)
    Use
    Donor plasmid
    Tags
    GFP, AID, PuroR
    Expression
    Mutation
    Inserting GFP AID 2A Puro into mouse 3' Ubf
    Promoter
    Available since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    127901-plasmid-map-sequence-id-249684

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  20. Myc-gamma1 (in pCAGGS)

    Plasmid
    #118039
    Purpose
    GABAA receptor expression (gamma1 subunit) Myc-tag near N-terminus
    Depositor
    Angel de Blas
    Insert
    GABRG1 (Gabrg1 Rat)
    Use
    Tags
    cMyc epitope EQKLISEEDL inserted between fourth a…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    118039-plasmid-map-sequence-id-228636

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  21. pBABE.zeo/hKGA.S286A

    Plasmid
    #110357
    Purpose
    Mammalian retroviral expression of glutaminase isoform KGA (S286A) with V5 tag
    Depositor
    Sandra Martha Gomes Dias
    Insert
    KGA glutaminase (GLS Human)
    Use
    Retroviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    S286A
    Promoter
    gag
    Available since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    110357-plasmid-map-sequence-id-213568

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  22. His6-Halo-TEV-Akt2

    Plasmid
    #87606
    Purpose
    expresses His-halo tagged at the N-terminus of human Akt2 protein
    Depositor
    Yimon Aye
    Insert
    AKT 2 (AKT2 Human)
    Use
    Zebrafish (danio rerio) expression
    Tags
    His-Halo-Tev
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    87606-plasmid-map-sequence-id-197920

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  23. Hs.KRAS4b M72C

    Plasmid
    #83160
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), M72C mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    M72C
    Promoter
    Available since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    83160-plasmid-map-sequence-id-158123

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  24. Hs.KRAS4b R73K

    Plasmid
    #83163
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), R73K mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    R73K
    Promoter
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83163-plasmid-map-sequence-id-158126

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  25. Hs.KRAS4b R73E

    Plasmid
    #83162
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), R73E mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    R73E
    Promoter
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83162-plasmid-map-sequence-id-158125

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  26. Hs.KRAS4b Y40F

    Plasmid
    #83161
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Y40F mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Y40F
    Promoter
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83161-plasmid-map-sequence-id-158124

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  27. Hs.KRAS4b Y40C

    Plasmid
    #83159
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Y40C mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Y40C
    Promoter
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83159-plasmid-map-sequence-id-158122

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  28. Hs.KRAS4b E37G

    Plasmid
    #83158
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), E37G mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    E37G
    Promoter
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83158-plasmid-map-sequence-id-158121

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  29. Hs.KRAS4b T35S

    Plasmid
    #83157
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), T35S mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    T35S
    Promoter
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83157-plasmid-map-sequence-id-158120

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  30. Hs.KRAS4b K147Q

    Plasmid
    #83155
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), K147Q mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    K147Q
    Promoter
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83155-plasmid-map-sequence-id-158118

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  31. Hs.KRAS4b K147L

    Plasmid
    #83154
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), K147L mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    K147L
    Promoter
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83154-plasmid-map-sequence-id-158117

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  32. Hs.KRAS4b Y64A

    Plasmid
    #83151
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Y64A mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Y64A
    Promoter
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83151-plasmid-map-sequence-id-158114

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  33. Hs.KRAS4b R68S

    Plasmid
    #83148
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), R68S mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    R68S
    Promoter
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83148-plasmid-map-sequence-id-158111

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  34. Hs.KRAS4b A146V

    Plasmid
    #83147
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), A146V mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    A146V
    Promoter
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83147-plasmid-map-sequence-id-158110

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  35. Hs.KRAS4b K117N

    Plasmid
    #83146
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), K117N mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    K117N
    Promoter
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83146-plasmid-map-sequence-id-158109

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  36. Hs.KRAS4b M188L

    Plasmid
    #83141
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), M188L mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    M188L
    Promoter
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83141-plasmid-map-sequence-id-158104

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  37. pJK598

    Plasmid
    #72523
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits, one with C-terminal His6 tag, and associated hypothetical protein (AaPurS, AaOrfY, AaPurQ, AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    72523-plasmid-map-sequence-id-132608

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  38. pJK612

    Plasmid
    #72533
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits; associated hypothetical protein N-terminal domain (AaPurS, AaOrfY[1-69], AaPurQ, AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    Expression
    Bacterial
    Mutation
    residues 1-69 only; C-terminal domain removed and…
    Promoter
    T7 and n/a
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72533-plasmid-map-sequence-id-132621

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  39. pJK613

    Plasmid
    #72534
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits, one with His6 tag; associated hypothetical protein N-terminal domain (AaPurS, AaOrfY[1-69], AaPurQ, AaPurLH6)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    residues 1-69 only; C-terminal domain removed and…
    Promoter
    T7 and n/a
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72534-plasmid-map-sequence-id-132623

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  40. pJK619

    Plasmid
    #72536
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit, N-term His-tag; hypothet. protein; glutaminase subunit; large subunit (H6AaPurS, AaOrfY, AaPurQ, AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72536-plasmid-map-sequence-id-132627

    Enlarge

Showing: 6601 - 6640 of 8371 results