We narrowed to 8,419 results for: BLI;

1. pBEST-p15a-OR2-OR1-Pr-UTR1-sigma28-T500

   Plasmid

   #45779

   Depositor

   Richard Murray, Vincent Noireaux

   Insert

   sigma factor 28
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   OR2-OR1-Pr (bacteriophage Lambda with one mutatio…

   Available since

   July 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB CAG-F-intron-L-F-bpA-SV40pA-L-td-sfGFP

   Plasmid

   #178433

   Purpose

   CAG tdGFP FLP/FRT cre/lox "NOT" reporter piggyBac transposon (bpA SV40pA stop)

   Depositor

   Mario Capecchi

   Insert

   td-sfGFP
   

   Use

   Cre/Lox; Flp/frt, piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBABE.zeo/TR

   Plasmid

   #110358

   Purpose

   Mammalian retroviral expression of Tetracycline repressor with β-globin intron for doxycycline inducible systems

   Depositor

   Sandra Martha Gomes Dias

   Insert

   Tetracycline repressor
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   gag truncated

   Available since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. FLAG-Cep215-FL

   Plasmid

   #106911

   Purpose

   Mammalian expression of CEP215

   Depositor

   Heidi Hehnly

   Insert

   CEP215 (Cdk5rap2 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCR2.1-hASIC1

   Plasmid

   #32790

   Depositor

   Cecelia Canessa

   Insert

   ASIC1 (ASIC1 Human)
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   plac

   Available since

   Nov. 1, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3_erGAP2

   Plasmid

   #78120

   Purpose

   Low affinity fluorescent calcium indicator of the GAP (GFP-Aequorin Protein) family targeted to the endoplasmic reticulum

   Depositor

   Teresa Alonso, Javier García-Sancho

   Insert

   erGAP2
   

   Use

   Tags

   KDEL and calreticulin signal peptide

   Expression

   Mammalian

   Mutation

   D24N/D119A in aequorin moiety

   Promoter

   CMV

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET His6 Sumo TEV cloning vector with BioBrick polycistronic restriction sites (9S)

   Plasmid

   #48291

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   His6 and Sumo

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. YIp204-PADH1-AFB2

   Plasmid

   #99531

   Purpose

   S. cerevisiae expression of F-box protein AFB2 under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)

   Depositor

   Helle Ulrich

   Insert

   AFB2 (AFB2 Mustard Weed)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUS252s

   Plasmid

   #191833

   Purpose

   Expresses fuGFPs constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuGFPs to place in other plasmid backbones

   Depositor

   Nicholas Coleman

   Insert

   fuGFPs
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 17, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pSV40-GUS-P2A-Hygro

    Plasmid

    #106480

    Purpose

    Expression of GUS and Hygromycin resistance gene from SV40 promoter

    Depositor

    Alex Hewitt

    Inserts

    beta-glucuronidase
    Hygromycin B resistance
    

    Use

    Tags

    Expression

    Mammalian and Plant

    Mutation

    Promoter

    SV40 promoter

    Available since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pxCas9CR4

    Plasmid

    #111656

    Purpose

    PCas9CR4 with the xCas9 mutations allowing NG PAM sites

    Depositor

    Christopher Reisch

    Insert

    TetR and xCas9
    

    Use

    Synthetic Biology

    Tags

    ssrA

    Expression

    Bacterial

    Mutation

    xCas9 mutations

    Promoter

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLENTI_TetOn_Hu_MAD2(WT)

    Plasmid

    #136347

    Purpose

    Lentiviral inducible expression (TetOn) of human MAD2

    Depositor

    Guillermo de Cárcer

    Insert

    MAD2L1 (MAD2L1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO.1-TRC.mKO2_shHuR CDS

    Plasmid

    #110426

    Purpose

    TRCN0000276129 (Target CGAGCTCAGAGGTGATCAAAG), silence human ELAVL1 (HuR) gene and express monomeric Kusabira-Orange2.

    Depositor

    Sandra Martha Gomes Dias

    Insert

    ELAVL1 HuR
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (RNA Pol III)

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. KDM4DL

    Plasmid

    #38990

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    KDM4DL (KDM4E Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

15. Pf-GMPS

    Plasmid

    #36899

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    Pf-GMPS (PF10_0123 Plasmodium falciparum)
    

    Use

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 19, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

16. PX396 Parvibaculum lavamentivorans Cas9

    Plasmid

    #68333

    Purpose

    Parvibaculum lavamentivorans Cas9

    Depositor

    Feng Zhang

    Insert

    PlCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. FFTTH--GFP-Amotl2

    Plasmid

    #112833

    Purpose

    transient/stable overexpression

    Depositor

    Tomasz Prószyński

    Insert

    Amotl2 (Amotl2 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. PIM1

    Plasmid

    #39132

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PIM1 (PIM1 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pTCF79 H-2D(b) (CD8 mut)

    Plasmid

    #180453

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    H-2D(b) (CD8 mut)
    

    Use

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    D227K, replacement of transmembrane domain with e…

    Promoter

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pExp-DsbA

    Plasmid

    #112562

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbA (leaderless thiol:disulfide interchange protein DsbA from E. coli) fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis-DsbA(AA20-208) and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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