We narrowed to 5,896 results for: dna plasmids

1. pFETCh_ARID4B

   Plasmid

   #101072

   Purpose

   Donor vector for 3' FLAG tag of human ARID4B

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   ARID4B homology arms (ARID4B Human)
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Available Since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFETCh_RERE

   Plasmid

   #101069

   Purpose

   Donor vector for 3' FLAG tag of human RERE

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   RERE homology arms (RERE Human)
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. PX458_RERE_1

   Plasmid

   #101073

   Purpose

   Encodes gRNA for 3' target of human RERE

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA against RERE (RERE Human)
   

   Use

   CRISPR

   Available Since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. Bxb1-GT-Dox-AIO-TetOn_ETV2_Down-Tandem

   Plasmid

   #241393

   Purpose

   Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression of ETV2

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   TRE-ETV2; rtTA-T2A-mTagBFP2 (ETV2 Human)
   

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   TRE; CAG

   Available Since

   Sept. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EFS-TadA8e-Nme2-U6-sgRNA scaffold

   Plasmid

   #226933

   Purpose

   Encoding Nme2ABE8e and sgRNA cassette on a single AAV

   Depositor

   Yuxuan Guo

   Article

   Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

   Insert

   TadA8e, nNme2Cas9
   

   Use

   AAV

   Promoter

   EFS

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-EFS-TadA8e-SaKKH-U6-sgRNA scaffold

   Plasmid

   #226935

   Purpose

   Encoding SaKKHABE8e and sgRNA cassette on a single AAV

   Depositor

   Yuxuan Guo

   Article

   Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

   Insert

   TadA8e, nSaKKHCas9
   

   Use

   AAV

   Promoter

   EFS

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. AA201

   Plasmid

   #216026

   Purpose

   Fragmid fragment: (Cas protein) firefly luciferase

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Fluc_v1.1
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. AA156

   Plasmid

   #216022

   Purpose

   Fragmid fragment: (Cas protein) Nanobody binds ALFA tag

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   NbALFA_v1.1
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. AA349

   Plasmid

   #216032

   Purpose

   Fragmid fragment: (Cas protein) Renilla luciferase

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Rluc_v1.1
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV6-AN-DDK_V222A RAD23B

    Plasmid

    #201580

    Purpose

    Expresses a variant of human RAD23B containing mutation V222A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog B (RAD23B Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains mutation V222A

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV6-AN-DDK_F400A RAD23B

    Plasmid

    #201581

    Purpose

    Expresses a variant of human RAD23B containing mutation F400A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog B (RAD23B Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains mutation F400A

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSCL.178

    Plasmid

    #184999

    Purpose

    Express -Eco1 FANCF editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: FANCF targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    FANCF donor, a1/a2 length extended for 27 bp

    Promoter

    H1

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSCL.176

    Plasmid

    #184997

    Purpose

    Express -Eco1 RNF2 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: RNF2 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    RNF2 donor, a1/a2 length extended for 27 bp

    Promoter

    H1

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSCL.179

    Plasmid

    #185000

    Purpose

    Express -Eco1 HEK4 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: HEK4 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    HEK4 donor, a1/a2 length extended for 27 bp

    Promoter

    H1

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. PX458_ARID4B_1

    Plasmid

    #101078

    Purpose

    Encodes gRNA for 3' target of human ARID4B

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against ARID4B (ARID4B Human)
    

    Use

    CRISPR

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. TBXTA-c043

    Plasmid

    #139766

    Purpose

    Protein expression/Streptavidin pull-down

    Depositor

    Opher Gileadi

    Insert

    TBXT, DNA-binding domain, WT (TBXT Human)
    

    Tags

    Avi-tag (Biotin) and His6-TEV

    Expression

    Bacterial

    Mutation

    Contains only amino acids E41- D225

    Promoter

    T7

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. TBXTA-c044

    Plasmid

    #139767

    Purpose

    Protein expression/Streptavidin pull-down

    Depositor

    Opher Gileadi

    Insert

    TBXT, DNA-binding domain, G177D (TBXT Human)
    

    Tags

    Avi-tag (Biotin) and His6-TEV

    Expression

    Bacterial

    Mutation

    G177D; contains only amino acids E41- D225

    Promoter

    T7

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTF-ahpC-rfp

    Plasmid

    #153035

    Purpose

    For CRISPR-Cas12a genome editing in E. coli. Contains a constituitively expressed CRISPR array targeting the ahpC gene in E. coli and donor DNA to insert an rfp translational fusion in the ahpC gene.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

    Insert

    CRISPR array
    

    Expression

    Bacterial

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTF-lacZ-rfp

    Plasmid

    #153037

    Purpose

    CRISPR-Cas12a genome editing in E. coli. Contains a constitutively CRISPR array targeting the lacZ gene in E. coli genome and donor DNA to insert an constitutively expressed rfp reporter gene.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

    Insert

    CRISPR array
    

    Expression

    Bacterial

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. GRLF1

    Plasmid

    #36195

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    GRLF1 (Codon Devices Synthesized) (ARHGAP35 Human)
    

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    Contains the Ras homolog domain only (amino acid …

    Available Since

    June 19, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits